FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23187088
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3577051.5426904836001831No Hit
ACCAGG3172161.3680717475174113No Hit
GTCTCA2934941.2657648084140622No Hit
AACATA2463411.062405939029515No Hit
CAGGAC2415721.0418384576795499No Hit
TCTTCA2160310.9316866352514814No Hit
GAAGAT2133810.9202578607542266No Hit
CGAGCC2110890.9103730490003746No Hit
GCTGTC2041190.8803132157000482No Hit
AACCTT1947340.8398381029993934No Hit
CATGAG1933540.8338865147706345No Hit
TGCATA1886270.8135001687145881No Hit
CAGCCT1879640.8106408187177278No Hit
CGTACT1869800.8063970775459169No Hit
CAACGG1856180.8005231187288374No Hit
GTAAGA1825140.7871363579592228No Hit
GGCCGT1820340.785066240314437No Hit
GCACAT1817200.7837120383551397No Hit
AAGCAT1786620.7705236638598172No Hit
TGTGAT1770490.7635672060243184No Hit
TAAGTC1714880.7395840305604567No Hit
AGTCAA1685390.7268657452803042No Hit
CGTCAG1658740.7153722796066501No Hit
AGTTAG1652210.7125560570607228No Hit
CTGGAG1640410.7074670178506245No Hit
TTCCAT1638640.7067036619691097No Hit
CATAAC1618280.6979229129591434No Hit
TGATAA1618030.6978150943318109No Hit
AGATGC1615630.696780035509418No Hit
AACAAT1603150.6913977296329751No Hit
ATGTGA1596480.6885211286557416No Hit
TTGAAT1593590.6872747453237767No Hit
AGTGCA1560480.6729952463198484No Hit
TTACGT1551100.6689498914223295No Hit
CCAGCG1547320.6673196737770608No Hit
ACACGA1533900.6615319698618473No Hit
CTGTTC1527430.6587416237864798No Hit
GAGATA1525240.6577971326110462No Hit
TATTCT1524670.657551306140728No Hit
TACGGC1524480.6574693639839553No Hit
GACACG1511500.6518714208528471No Hit
ATCCAC1503160.6482745914450319No Hit
CCTTAT1487630.6415768983151313No Hit
AATCGG1484750.6403348277282598No Hit
CAGTCC1475470.6363326002816739No Hit
CCGTCG1474790.6360393336153294No Hit
GTTACA1451860.6261502091163841No Hit
GTGGTG1444700.6230622836295787No Hit
TATAAG1424610.6143979787371316No Hit
GCTCAA1403970.6054964728645529No Hit
ATTGCT1389280.5991610503224898No Hit
ATTCAT1383280.5965734032665077No Hit
TATGGT1380530.5953873983658492No Hit
GGACTA1364870.5886336395497356No Hit
GTCTGC1364250.5883662493539508No Hit
CCTCGG1359100.5861451856308995No Hit
CCACTT1357020.5852481346514923No Hit
GCTTCT1356640.5850842503379466No Hit
TGCGAC1354590.5842001375938195No Hit
CCGACT1350660.5825052287721512No Hit
GCCATA1349160.5818583170081556No Hit
GCATAA1348580.581608177792744No Hit
CTGTAT1346060.5805213660292314No Hit
AAGCGC1340650.5781881709337542No Hit
CTTAGC1331060.5740522483892759No Hit
CAAGGA1328360.5728878072140839No Hit
TCATGT1312960.5662461797703964No Hit
GAGCAC1304000.5623819601667962No Hit
ATCCGG1303250.5620585042847984No Hit
ATGCCG1300520.5608811248743266No Hit
GAGTGA1299380.5603894719336899No Hit
TTCTGT1290650.5566244454672359No Hit
AGGACT1286010.554623331743943No Hit
GACTAA1279280.5517208542961496No Hit
CCATGA1277350.550888494493142No Hit
GACCAT1276160.5503752778270389No Hit
TCCACG1275470.550077698415601No Hit
ACAGCC1271700.5484517935154255No Hit
TAATGA1265890.5459460886162161No Hit
AAGTTG1265080.5455967562636584No Hit
CGAATT1251490.5397357356818588No Hit
CGTGAA1245370.537096335684757No Hit
GCGTAT1234130.5322488101998837No Hit
AATTGC1232200.531416450396876No Hit
GTACTT1226150.5288072396154274No Hit
ACCGCT1205380.5198496680566356No Hit
AGTTCC1199970.5175164729611584No Hit
GAGAAG1199620.5173655268828928No Hit
AGCCTA1192530.5143077906117405No Hit
GGTGGT1186410.5116683906146386No Hit
GGCGTT1185240.511163799438722No Hit
AGAACG1182960.5101804935574489No Hit
TCTCTG1174690.5066138533652867No Hit
CCTGCT1171350.5051733965041234No Hit
TTCACA1171120.5050742033669773No Hit
GAATAC1168880.5041081484660773No Hit
TACAGT1161380.5008735896460996No Hit
AGGTGT1156520.498777595530754No Hit
AGTAGG1148160.4951721406327521No Hit
CACCTA1146430.4944260357316106No Hit
TCCTCC1143350.4930977102428731No Hit
TACGAA1141020.49209284063613334No Hit
TTCTAC1138580.49104053083336724No Hit
GGAGGC1132700.4885046367185047No Hit
TCAAGC1126580.4858652367214029No Hit
GCGCAG1123880.4847007955462109No Hit
ACGAAC1121960.48387274848829653No Hit
CTACCG1108640.4781281720240161No Hit
TACTCG1104240.47623056418296256No Hit
GTCGAG1102340.47541114261523476No Hit
ATTGAA1100090.4744407749692415No Hit
CAGTAG1097160.4731771406569035No Hit
ACGTAA1096910.4730693220295709No Hit
TTAGTA1096820.4730305073237312No Hit
CGACAA1096790.47301756908845133No Hit
AGACGG1091530.47074906516937354No Hit
ACTAGC1089500.46987357791543294No Hit
GACTTG1088090.46926548085727715No Hit
GTACGC1080920.4661732426253784No Hit
CTTCAC1059180.45679733479253626No Hit
ACTAAT1053310.45426575342276704No Hit
CACATT1043800.4501643328390353No Hit
GGCCAG1041550.4491939651930419No Hit
GAGTCG1040260.44863762107600574No Hit
CTAGTT1028980.4437728446107592No Hit
TGTGTG1028030.4433631338268954No Hit
GGATTC1022040.44077979951600654No Hit
TAGAGC1015960.43815765049927785No Hit
TGCGGA982860.4238824642404428No Hit
TCAATG977750.4216786514977647No Hit
GTTGGC973940.420035495617216No Hit
AGGTCA973120.4196818505195651No Hit
AAGGCG967830.4174004083652074No Hit
ATTCGA962120.41493783091693104No Hit
AGGCTT955660.4121517975866569No Hit
GAACCG949030.40929244758979655No Hit
CAAGCT941270.4059457573973929No Hit
TCGTGG935640.403517681909863No Hit
GACCGA932150.40201253387229996No Hit
CGAAGC930000.4010852936772397No Hit
TCTCGC929400.40082652897164145No Hit
CTCGTC927020.39980009563943514No Hit
ATGGCC923300.39819575446472627No Hit
CTCCGC922200.3977213525044629No Hit
TGACAC921200.39729007799513244No Hit
CGTTGC906050.3907562691787774No Hit
ATACCT890040.38385156428439826No Hit
CCTGAC879470.3792929927207763No Hit
CGCTTG861270.37144379665096366No Hit
CCGGAA845520.3646512231290104No Hit
GGCAGG842790.3634738437185385No Hit
CATACG841240.3628053682290765No Hit
ACCTGC840330.3624129084255858No Hit
TTCGTT819590.353468275102074No Hit
AAGAGG819530.3534423986315143No Hit
GGTAAT819430.3533992711805812No Hit
TGGCAT818600.353041313337837No Hit
ACGCTA816790.35226070647594904No Hit
ATCAGC809600.3491598427538637No Hit
TCCAGA797860.3440966800143252No Hit
CTATTA774260.3339186015941286No Hit
TTAGCG772910.3333363810065326No Hit
TATTGG760300.3278980094438767No Hit
AACTCT757740.32679394669999096No Hit
CCGCTC757590.3267292555235914No Hit
CATTAA735770.31731884573000285No Hit
TCGGAC726450.31329936730304386No Hit
CGTCTC713560.3077402388777754No Hit
AACTAG711400.30680868593762184No Hit
GCCTGG697400.30077084280699673No Hit
CTGAGG689440.297337897712727No Hit
AATGTA673130.29030381046554876No Hit
GATTCA670400.28912643105507685No Hit
AGAGGT646240.27870683890965525No Hit
CTATGC641480.2766539722452427No Hit
CGAGAG621490.26803279480372866No Hit
GCGGCT611890.2638925595141572No Hit
GGATCG601170.2592692967741357No Hit
GATCCT595230.2567075261887133No Hit
GAGCCA497000.21434343113719154No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)