FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29714688
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4528431.5239702331722278No Hit
ACCAGG4027951.3555417442040785No Hit
GTCTCA3705201.2469254262403833No Hit
AACATA3119641.049864632601897No Hit
CAGGAC3101541.0437733689143902No Hit
TCTTCA2720040.9153856840092012No Hit
CGAGCC2694340.906736762640752No Hit
GAAGAT2638320.887884133260965No Hit
GCTGTC2594370.8730934681192009No Hit
AACCTT2476390.8333891979616277No Hit
CATGAG2416430.8132106249946154No Hit
CAGCCT2391710.8048915068534456No Hit
TGCATA2383390.8020915447606247No Hit
CGTACT2356150.7929243611778795No Hit
CAACGG2351680.791420054620799No Hit
GCACAT2309140.7771039022856305No Hit
GGCCGT2303720.7752798885184323No Hit
AAGCAT2273480.7651031032195257No Hit
GTAAGA2261700.7611387338140653No Hit
TGTGAT2188880.7366323348237747No Hit
TAAGTC2132550.7176753799333178No Hit
AGTCAA2104530.7082456999043706No Hit
CGTCAG2077480.6991424577636488No Hit
AGATGC2068210.6960227884607101No Hit
CATAAC2058760.6928425430548018No Hit
CTGGAG2052810.6908401663177484No Hit
TTCCAT2048670.6894469159494456No Hit
TGATAA2032020.6838436264247499No Hit
AACAAT2028480.6826522963996795No Hit
ATGTGA2013100.6774764049348254No Hit
TACGGC2007460.6755783537084421No Hit
AGTTAG1999980.6730610800961464No Hit
ACACGA1961180.6600035645671258No Hit
CCAGCG1949440.6560526565178809No Hit
TTGAAT1948880.6558641975308641No Hit
AGTGCA1948570.6557598720201941No Hit
CTGTTC1941830.653491633497885No Hit
TTACGT1937180.6519267508378348No Hit
ATCCAC1911870.6434090776924867No Hit
GACACG1899530.6392562493000095No Hit
GAGATA1899000.6390778863301543No Hit
TATTCT1889980.6360423505035624No Hit
CAGTCC1867980.6286386045850456No Hit
CCTTAT1865400.6277703471091469No Hit
AATCGG1860390.6260843122431573No Hit
CCGTCG1856030.6246170244156695No Hit
GTGGTG1790280.6024899201364659No Hit
GTTACA1787840.6016687774073213No Hit
GCTCAA1783990.6003731218715809No Hit
AAGCGC1746950.5879079060160416No Hit
GTCTGC1745550.5874367585484996No Hit
TATAAG1743350.586696383956648No Hit
TGCGAC1741600.5861074496222205No Hit
GCATAA1728990.5818637570752888No Hit
ATTCAT1720440.5789863921842289No Hit
GCCATA1713420.5766239241684112No Hit
CCTCGG1713240.5765633480654415No Hit
CCACTT1713090.5765128679796335No Hit
GCTTCT1710830.5757523013534587No Hit
CCGACT1707430.5746080860751424No Hit
CTTAGC1703630.5733292572346713No Hit
CAAGGA1701460.5725989786599812No Hit
GGACTA1700060.5721278311924393No Hit
TATGGT1686470.5675543354182282No Hit
CTGTAT1685890.5673591457531036No Hit
ATTGCT1666010.5606688517140076No Hit
GAGCAC1654060.5566472715446313No Hit
ATCCGG1645720.5538405787737027No Hit
TCATGT1636820.5508454270157572No Hit
GAGTGA1630320.548657956630741No Hit
ACAGCC1626880.5475002799962093No Hit
GACCAT1625140.5469147110008357No Hit
GACTAA1622320.5459656853876439No Hit
CCATGA1620520.5453599243579471No Hit
ATGCCG1619990.5451815613880919No Hit
TTCTGT1613660.5430513017669915No Hit
TCCACG1599930.5384306912460262No Hit
CGAATT1581130.5321038538247482No Hit
TAATGA1578440.5311985776192568No Hit
CGTGAA1573980.5296976364012302No Hit
AGGACT1568610.5278904493293014No Hit
AATTGC1567640.5275640114410759No Hit
GCGTAT1556640.5238621384818174No Hit
AAGTTG1546580.520476607393623No Hit
ACCGCT1533370.516030994503459No Hit
GTACTT1530050.5149137019375738No Hit
AGCCTA1506860.5071094806716463No Hit
CCTGCT1500310.5049051835913606No Hit
GGCGTT1497610.5039965420468153No Hit
GAATAC1483470.4992379526246414No Hit
TCTCTG1478400.49753172572432863No Hit
AGTTCC1476360.4968451965573389No Hit
GAGAAG1473280.4958086721287466No Hit
AGAACG1468770.49429090421545063No Hit
TCCTCC1467440.49384331412128574No Hit
TTCACA1461960.4919991083197643No Hit
TACAGT1461930.49198901230260267No Hit
GGTGGT1454730.48956596818381537No Hit
CACCTA1449790.487903490691203No Hit
TACGAA1441180.4850059337658198No Hit
GGAGGC1435870.4832189387282141No Hit
ACGAAC1432970.48224299040259144No Hit
TTCTAC1427660.48045599536498584No Hit
AGGTGT1421650.4784334265936092No Hit
TCAAGC1414660.4760810545949532No Hit
AGTAGG1401070.47150755882074213No Hit
AGACGG1399150.4708614137223988No Hit
GCGCAG1397230.4702152686240556No Hit
CTACCG1385210.4661701310813023No Hit
GTCGAG1384830.4660422481972552No Hit
ACGTAA1382960.46541292979418125No Hit
ACTAGC1379960.4644033280780198No Hit
TACTCG1379010.4640836208679021No Hit
CGACAA1378230.46382112442170015No Hit
GTACGC1378030.46375381764062273No Hit
CAGTAG1358480.45717457979030435No Hit
CTTCAC1348740.45389673955183374No Hit
GACTTG1346950.45329434386119083No Hit
ATTGAA1342470.45178667196505645No Hit
ACTAAT1315960.44286515813324373No Hit
GGCCAG1313840.44215170625382305No Hit
CACATT1309710.4407618212245742No Hit
CTAGTT1296320.43625563223144054No Hit
TGTGTG1281100.4311335861914485No Hit
GAGTCG1280210.43083407101565396No Hit
TAGAGC1267370.4265129756704832No Hit
TGCGGA1258450.4235110932344301No Hit
GGATTC1231650.4144919845700551No Hit
GTTGGC1221080.4109348211901131No Hit
TCAATG1215460.4090435006418374No Hit
AGGCTT1203290.40494788301327617No Hit
AGGTCA1202990.40484692284165996No Hit
ATTCGA1202220.40458779173451187No Hit
AAGGCG1199810.40377674502252897No Hit
CGAAGC1195040.4021714782938324No Hit
TTAGTA1194070.4018450404056068No Hit
GACCGA1191210.4008825534361996No Hit
CAAGCT1190010.40047871274973507No Hit
CATACG1186090.39915949984061755No Hit
ATGGCC1182480.39794461244216994No Hit
TCGTGG1180930.39742298488881994No Hit
GAACCG1176920.39607348392821756No Hit
CTCGTC1176070.3957874301086385No Hit
TCTCGC1175160.3954811842547363No Hit
CTCCGC1173540.39493599932800916No Hit
TGACAC1168680.3933004445478277No Hit
CGTTGC1152910.38799330485987266No Hit
ATACCT1127130.37931746077899253No Hit
CCTGAC1119500.3767497070808888No Hit
CGCTTG1086590.36567437625459837No Hit
CCGGAA1081520.3639681493542857No Hit
ACCTGC1077670.3626724938185452No Hit
GGCAGG1069750.36000714528787914No Hit
TGGCAT1056180.3554403801917759No Hit
ACGCTA1040060.35001545363693537No Hit
ATCAGC1031100.3470001098446667No Hit
TTCGTT1024410.3447486980176268No Hit
TCCAGA1017590.3424535367828866No Hit
AAGAGG1008410.3393641555314328No Hit
GGTAAT1002680.33743581625356456No Hit
CCGCTC969650.32632010135862777No Hit
CTATTA969340.32621577584795775No Hit
AACTCT959380.322863898150302No Hit
TATTGG940840.31662455954442464No Hit
CATTAA930060.3129967240443514No Hit
TCGGAC910740.3064948889922721No Hit
CGTCTC908640.30578816779095913No Hit
TTAGCG886430.29831374975231106No Hit
AACTAG885620.2980411572889475No Hit
GCCTGG882020.2968296352295538No Hit
CTGAGG865440.2912499030782353No Hit
NNNNNN849880.2860134355104116No Hit
GATTCA831730.2799053451276352No Hit
AATGTA824870.2775967225366795No Hit
GCGGCT820210.2760284745375755No Hit
CTATGC810130.27263621277127326No Hit
AGAGGT793050.2668882136672611No Hit
CGAGAG762720.25668114031686956No Hit
GATCCT752710.2533124359239444No Hit
GGATCG724620.24385919852162002No Hit
GAGCCA623650.20987937009468177No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)