FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30007842
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGG6282852.0937360307348993No Hit
GTCTCA4972741.6571468218207759No Hit
AACATA4741901.5802202637563874No Hit
TGATAA4342351.4470717354483538No Hit
GCTGTC3901231.300070161659742No Hit
TATAAG3855601.284864136514715No Hit
ATTCAT3779781.2595974079042405No Hit
TGTGAT3775031.2580144883460798No Hit
CATGAG3737561.2455277523788615No Hit
GTCTGC3717141.2387228645098838No Hit
CTGTAT3659621.2195545417761129No Hit
TTCTGT3478221.1591036769655079No Hit
GACCAT3423781.1409617525978708No Hit
GGTACC3232571.0772417423418852No Hit
GTGGTG3154251.0511418981744838No Hit
ATTGAA2980150.9931237307901047No Hit
AGTTAG2960690.9866387592949869No Hit
ATTGCT2960180.9864688037213739No Hit
GGCCGT2957220.9854823949019726No Hit
GACACG2953790.9843393603578691No Hit
CCACTT2935460.9782309570944823No Hit
ACTAAT2830400.9432201089301923No Hit
TCATGT2822770.9406774402504519No Hit
CTAGTT2725240.9081759361436255No Hit
CCGTCG2670690.8899973546914837No Hit
TTCCAT2666340.8885477336224311No Hit
CAGTCC2616080.8717987784659758No Hit
AATTGC2579510.8596119640992512No Hit
AGTGCA2529500.8429463204984883No Hit
GCACAT2523040.8407935498993897No Hit
TTCTAC2466540.821965138312845No Hit
GCCATA2394160.7978447767087017No Hit
GACTAA2369400.789593600232899No Hit
ATACCT2365610.7883305970485982No Hit
GCGTAT2266870.7554258650122192No Hit
CAAGGA2258930.7527798900034198No Hit
AGATGC2216150.7385236165932891No Hit
AAGCGC2201910.7337781903810344No Hit
CAGGAC2184280.7279030594735869No Hit
CTTAGC2174080.7245039480013258No Hit
CATAAC2166340.7219246222370805No Hit
TCCTCC2151040.7168259550286888No Hit
ATCAGC2136470.7119705575629197No Hit
GTACTT2072640.6906994511634659No Hit
GCTTCT2035840.678435990165504No Hit
ATTCGA2003790.6677554487257031No Hit
AGTTCC1961760.6537491099826505No Hit
GAGCAC1946230.6485737961430215No Hit
TACAGT1945850.6484471625783687No Hit
GACTTG1942350.6472808007986712No Hit
AGGACT1920470.6399893734444483No Hit
GAAGAT1893780.6310950317586983No Hit
CGAGCC1891220.6302419214284052No Hit
AAGTTG1859940.6198179795801377No Hit
GTTGGC1845700.6150725533678829No Hit
CGCTTG1831090.610203826053203No Hit
GTCGAG1813480.604335360070211No Hit
AGGTGT1808020.6025158356938829No Hit
TACGAA1795940.5984902213228129No Hit
TGCGGA1783140.5942246696713479No Hit
CTTCAC1769320.5896192068726569No Hit
CGACAA1765790.5884428477062762No Hit
CCATGA1758070.5858701868664864No Hit
TACTCG1730390.5766459314201934No Hit
TAGAGC1689780.5631128023134753No Hit
AACAAT1665190.5549182776955437No Hit
CGAAGC1641890.5471536407049864No Hit
CCTGCT1630070.5432146703518368No Hit
ACTAGC1620700.5400921532444752No Hit
CCTTAT1605670.5350834625162315No Hit
TCTTCA1545810.5151353436211774No Hit
GAACCG1530570.5100566711861519No Hit
CACCTA1521770.5071241044257698No Hit
CTGAGG1508530.5027119244362858No Hit
TGACAC1502870.5008257508154035No Hit
TGTGTG1499150.4995860748666965No Hit
TTAGTA1484380.4946640281563733No Hit
GCCTGG1482540.4940508551064752No Hit
TCAATG1468910.48950870909011057No Hit
CCTGAC1446730.4821173078690563No Hit
CTCCGC1444750.4814574803479704No Hit
TCTCGC1434120.47791507299991787No Hit
ATGCCG1429820.4764821142420038No Hit
GGCAGG1408620.469417294319265No Hit
CCGACT1396140.4652583814590866No Hit
GATTCA1379410.4596831721521328No Hit
CCGCTC1372140.4572604721125898No Hit
ACACGA1363470.45437122736116775No Hit
CGTTGC1337320.4456568386357139No Hit
GACCGA1334840.4448303880032426No Hit
CGTACT1311340.43699910176813117No Hit
TCGTGG1299730.4331301131217633No Hit
GGTAAT1298410.43269022810770597No Hit
AGGCTT1288880.42951439160470123No Hit
TCCAGA1288120.4292611244753955No Hit
AACTCT1286580.4287479252923286No Hit
GAATAC1274420.42469565122343683No Hit
TTAGCG1253590.41775413240312315No Hit
CAGCCT1253340.4176708208474305No Hit
CAAGCT1245390.4150215133764034No Hit
AGCCTA1229300.40965958165202276No Hit
GGCGTT1222160.40728020362144No Hit
GGTGGT1202420.4007019231839464No Hit
CAGTAG1180830.3935071372343269No Hit
CAACGG1179290.3929939380512601No Hit
TTCGTT1174070.39125439276839696No Hit
TCCACG1151200.3836330516536311No Hit
TCGGAC1149880.38319316663957376No Hit
GGCCAG1142030.3805771837908237No Hit
AGACGG1141160.38028725957701326No Hit
GCGGCT1139550.3797507331583524No Hit
AAGAGG1138850.3795174608024129No Hit
ACCTGC1119780.37316245533417564No Hit
CCAGCG1117430.3723793267106645No Hit
ATGTGA1113180.37096303026388905No Hit
CGTCTC1106480.36873028057132534No Hit
CGTCAG1092470.3640615009903078No Hit
TTGAAT1059350.3530243860921422No Hit
CATTAA1034480.34473655253183483No Hit
TTACGT1032410.34404673285069953No Hit
ATGGCC1012380.33737181100860236No Hit
AGAGGT1005490.3350757445337122No Hit
GCTCAA992350.3306968891665052No Hit
AATGTA942910.31422119591272174No Hit
TGCGAC927400.3090525469975482No Hit
TACGGC923250.30766957517304977No Hit
GTAAGA866180.28865121323952586No Hit
CCTCGG855080.2849521801667711No Hit
GTTACA848110.28262945399405925No Hit
AGAACG842950.28090990348456246No Hit
CGTGAA823200.2743282905848411No Hit
GCGCAG811980.2705892679653538No Hit
TTCACA807130.26897302378491594No Hit
GAGAAG806050.2686131178643236No Hit
AACCTT804530.26810658360571216No Hit
CTGGAG799900.2665636535942838No Hit
GCATAA794610.2648007810758268No Hit
TATGGT793560.26445087254191757No Hit
ACAGCC784920.2615716251771787No Hit
AGTCAA784620.26147165131034744No Hit
TGCATA773200.2576659794463061No Hit
GAGTGA771610.2571361179521006No Hit
CTACCG765000.2549333604195863No Hit
TCTCTG760390.2533970953326134No Hit
AAGCAT730170.2433263944804828No Hit
GGAGGC721630.24048047173802103No Hit
GAGATA719450.2397539949723809No Hit
ATCCAC705710.23517519187151145No Hit
TATTCT692560.2307930040420767No Hit
GTACGC682820.22754718583229008No Hit
TAAGTC680690.2268373713777885No Hit
AATCGG679360.22639415390150347No Hit
AGTAGG671980.2239347967774557No Hit
AAGGCG654710.2181796345102057No Hit
GGACTA654710.2181796345102057No Hit
AGGTCA648820.21621681425808628No Hit
GAGTCG648690.21617349224912608No Hit
ACGTAA642100.21397739964106716No Hit
CTGTTC631610.21048164676420253No Hit
TATTGG621570.2071358546875847No Hit
CTATTA611490.203776732762056No Hit
CTCGTC602260.20070087012588242No Hit
CCGGAA597830.19922458935900822No Hit
CGAATT593930.19792492909020248No Hit
ATCCGG546830.18222903199770246No Hit
ACCGCT540050.1799696226073171No Hit
ACGCTA527320.1757273981914461No Hit
CACATT497270.16571334919718653No Hit
CGAGAG487690.1625208503830432No Hit
TAATGA487360.1624108791295289No Hit
GGATCG472050.15730887945890945No Hit
GATCCT468750.15620916692376613No Hit
ACGAAC457320.152400162597497No Hit
GGATTC453790.15122380343111644No Hit
TCAAGC442030.147304827851333No Hit
AACTAG439820.14656835369900975No Hit
GAGCCA391520.1304725611391849No Hit
CATACG366140.12201477200526449No Hit
TGGCAT307200.10237323963515937No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)