FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26451894
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT5669412.1432907602003852No Hit
ACCAGG5417712.048136893335502No Hit
AACTCT4372481.6529931656311643No Hit
ATTCAT3950831.4935905912824239No Hit
GGTAAT3359031.269863700497212No Hit
TTCTAC3338711.2621818309116164No Hit
ACTAGC3305421.2495967207489944No Hit
ATCCGG3304621.2492942849385378No Hit
CCGTCG3093791.1695911075403524No Hit
CGTTGC2903981.097834431061912No Hit
CCATGA2717051.027166523501115No Hit
TAGAGC2588450.978549966970229No Hit
AGTGCA2496580.9438189945869282No Hit
AATCGG2473980.9352751829415316No Hit
CGAATT2446830.9250112676241633No Hit
CGTACT2293890.8671931015601378No Hit
CAAGGA2235610.8451606527683801No Hit
GCATAA2228710.8425521439031927No Hit
TCTTCA2197470.8307420255048655No Hit
GGTACC2181760.8248029422770257No Hit
CCACTT2157030.8154538952862883No Hit
AACATA2140430.8091783522193154No Hit
CTATTA2121460.8020068430638653No Hit
AACTAG2117820.8006307601262881No Hit
CCTTAT2037100.770114986851225No Hit
AGACGG1979630.7483887543175547No Hit
TACGAA1975500.7468274294460729No Hit
CCTGCT1865790.7053521384895918No Hit
GATCCT1851920.7001086576258018No Hit
TGTGAT1844190.6971863716072656No Hit
ACCTGC1830100.6918597208956001No Hit
GCACAT1828520.6912624101699485No Hit
TACGGC1779770.6728327279702543No Hit
GTCTCA1761050.6657557300055716No Hit
CAGGAC1742590.6587770236792874No Hit
TGGCAT1724570.6519646570487542No Hit
CCTCGG1721960.6509779602171398No Hit
GCTGTC1698160.6419804948560582No Hit
TTCTGT1672580.632310109816711No Hit
TTAGTA1664950.629425628274482No Hit
ATGGCC1658200.6268738261237551No Hit
GCTCAA1650130.6238230048857749No Hit
ACAGCC1649060.6234184969892893No Hit
AGGCTT1646120.6223070453858617No Hit
GTACTT1626250.6147952959436477No Hit
TCGTGG1612870.6097370570137625No Hit
GTGGTG1603480.6061872166885289No Hit
GAGTCG1602150.6056844171536451No Hit
GTTGGC1593150.6022820142860091No Hit
ATCCAC1587630.6001952071938592No Hit
CTGGAG1567980.5927666275995208No Hit
AGTCAA1545080.5841094025252029No Hit
GTTACA1543070.583349532551431No Hit
TATTCT1537810.5813610170976793No Hit
CACCTA1520960.5749909628399388No Hit
CATGAG1516770.5734069552826727No Hit
TAAGTC1515330.5728625708238511No Hit
GGACTA1486100.5618123223992959No Hit
AAGCAT1485250.5614909843506859No Hit
CACATT1479850.5594495426301043No Hit
TATTGG1476230.5580810205877885No Hit
AGGACT1461340.5524519340656665No Hit
ACTAAT1461100.5523612033225296No Hit
CATTAA1459030.5515786506629733No Hit
AGCCTA1458660.5514387741006371No Hit
TCCAGA1456780.5507280499460644No Hit
TCCTCC1455250.5501496414585663No Hit
TTCCAT1454280.5497829380383877No Hit
GAAGAT1424040.5383508644031312No Hit
GACCAT1423840.5382752554505171No Hit
CGTCAG1420810.5371297798184129No Hit
GAGTGA1414070.5345817581153168No Hit
GAGCAC1410420.5332018947301089No Hit
TACAGT1392330.5263630649661608No Hit
AACCTT1390010.525486001115837No Hit
GTAAGA1379840.5216412858754084No Hit
CGAGAG1377450.5207377588916695No Hit
CAGTAG1361030.5145302638820494No Hit
TGCATA1358630.5136229564506799No Hit
CTGAGG1352910.5114605404059157No Hit
GCTTCT1350960.5107233531179279No Hit
AGATGC1346080.5088784946741431No Hit
GAGATA1335120.5047351240708888No Hit
TATAAG1330480.502980996370241No Hit
GACCGA1319640.49888299113855517No Hit
GGAGGC1314340.49687935389428073No Hit
TATGGT1300210.4915375813920924No Hit
TCGGAC1293410.48896687700321195No Hit
ATTGCT1279970.48388595538754237No Hit
ACCGCT1274060.48165171083779484No Hit
TGTGTG1272910.48121695936026354No Hit
GGTGGT1269200.4798144132892715No Hit
AATTGC1267960.4793456377830639No Hit
AGTAGG1266160.47866515720953673No Hit
CATAAC1262300.47720590442408395No Hit
CTGTAT1248870.47212876325604514No Hit
AATGTA1224450.46289691014185974No Hit
GACACG1221650.46183838480526196No Hit
ATCAGC1219700.4611011975172742No Hit
CGACAA1217770.46037157112454785No Hit
CGAAGC1217120.46012584202855195No Hit
CAAGCT1216160.4597629190560041No Hit
TTCACA1212590.4584132992518418No Hit
GGCGTT1205490.45572918143404023No Hit
ACACGA1177320.44507966045833997No Hit
CCGACT1163840.4399836170521476No Hit
TTCGTT1159000.43815388039888564No Hit
CTATGC1156960.43738266908222145No Hit
GGCCGT1156420.4371785249101633No Hit
CTTAGC1143430.43226772343787556No Hit
CTCCGC1134040.42871788311264214No Hit
CGAGCC1126460.4258523038085666No Hit
CAGTCC1115090.4215539348524533No Hit
CGTGAA1108590.41909664389249407No Hit
TCTCTG1105450.41790958333645223No Hit
CTAGTT1103910.4173273944013234No Hit
GACTAA1098040.4151082716420986No Hit
AACAAT1093800.41350536184667913No Hit
GGATTC1087210.4110140468580435No Hit
ATTCGA1076720.40704835729343236No Hit
ATGCCG1075110.40643970522488865No Hit
ACGTAA1070860.4048330149818384No Hit
GTACGC1061650.4013512227139577No Hit
AGGTGT1040410.393321551946337No Hit
GGATCG1038200.3924860730199509No Hit
TGATAA1038200.3924860730199509No Hit
GGCCAG1036840.3919719321421748No Hit
ACGAAC1034810.3912045012731413No Hit
GTCGAG1023900.38708003290804055No Hit
TAATGA1013790.38325800035339624No Hit
AGGTCA1008340.3811976563946612No Hit
ATTGAA1005170.37999925449572725No Hit
TTGAAT1004040.37957206391345744No Hit
CGTCTC950530.3593428886415468No Hit
TCAAGC946970.35799704928501525No Hit
AGAGGT931770.3522507688863414No Hit
TCATGT930410.35173662800856526No Hit
CTTCAC921680.3484362972269585No Hit
GAGCCA919390.3475705747195267No Hit
TACTCG918570.3472605780138088No Hit
GCGGCT906770.34279964980957506No Hit
GCCATA891150.3368945906104115No Hit
TCCACG867410.3279198079351142No Hit
GATTCA866980.32775724868699385No Hit
TGCGGA864710.32689908707482346No Hit
CTGTTC862960.3262375087394498No Hit
ATGTGA855580.3234475383879884No Hit
GCCTGG845730.31972379747174245No Hit
TCTCGC841530.3181360094668457No Hit
CAGCCT832260.31463153451318077No Hit
GAACCG826530.31246533802078597No Hit
ATACCT818440.3094069558875444No Hit
GTCTGC809240.30592894406729443No Hit
GACTTG806210.3047834684351903No Hit
GCGTAT805160.3043865214339661No Hit
CATACG804460.30412189009981666No Hit
AAGCGC784320.29650806857157375No Hit
GAGAAG780040.2948900369856314No Hit
CCAGCG745570.281858834002586No Hit
CTACCG744700.2815299350587145No Hit
ACGCTA726920.2748082991713183No Hit
GCGCAG712790.26946652666912996No Hit
TTAGCG711860.2691149450394743No Hit
AAGGCG688260.26019308863100693No Hit
CGCTTG680170.2571347064977653No Hit
TGCGAC645730.2441148448576121No Hit
AGAACG638950.24155170136399307No Hit
CCGGAA608700.23011584728110582No Hit
AGTTCC581390.21979144480164634No Hit
CTCGTC575010.21737951921325555No Hit
GGCAGG504670.19078785057886594No Hit
GAATAC483950.182954763088042No Hit
CCTGAC459460.17369644684044175No Hit
TGACAC423630.1601511029796203No Hit
NNNNNN400100.15125570970456784No Hit
AGTTAG377720.14279506790704666No Hit
AAGAGG356560.13479564072047165No Hit
CAACGG318960.12058115762901514No Hit
TCAATG270790.10237074139190185No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)