FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28954366
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT16939145.850288692213119No Hit
ATTCAT6134292.118606223323971No Hit
GGTACC5869502.027155421051181No Hit
CTATTA5750831.9861702376767636No Hit
GATCCT5014391.7318251762100403No Hit
CAGGAC4631981.5997518301730387No Hit
CGTCAG4254441.4693604411852774No Hit
CGTACT4125201.424724685734787No Hit
TATTGG4013321.3860845718397012No Hit
TCTTCA3974541.3726910822360952No Hit
GGAGGC3878051.3393662289134565No Hit
GAAGAT3877791.3392764324385484No Hit
CGAGAG3705991.2799416847877105No Hit
AACTCT3590521.2400616887967777No Hit
CCGTCG3525661.217660922017771No Hit
TCTCTG3319441.1464385025733252No Hit
CGTGAA3301021.140076767697141No Hit
GGTAAT3182281.0990674083487098No Hit
ACACGA3170091.0948573351597477No Hit
GTACGC3165111.0931373872941994No Hit
TTGAAT3022321.0438218540167656No Hit
CGAGCC3013901.0409138297139713No Hit
ATCCGG2965451.0241806019858974No Hit
GCTCAA2941901.0160471135855644No Hit
AGTGCA2939441.0151975007845104No Hit
GGATCG2865610.989698755621173No Hit
TTCTGT2600360.8980890826620067No Hit
CCTTAT2551090.8810726506669149No Hit
GAGCCA2524800.871992845569473No Hit
CAGCCT2470220.8531424932599112No Hit
AGACGG2433890.8405951627467857No Hit
GAGAAG2344200.809618832614052No Hit
TCCACG2336810.8070665405003169No Hit
TACGAA2307220.7968470109136563No Hit
ACCAGG2273020.785035320752663No Hit
ACTAAT2256340.7792745315162487No Hit
ACGCTA2218190.7660986256787664No Hit
TTCACA2204350.7613186902451948No Hit
GACCAT2202900.7608179022120533No Hit
TAGAGC2089050.7214974073340097No Hit
TATAAG2069520.7147523105841792No Hit
AAGGCG2063040.7125143061326227No Hit
ATGGCC2023880.698989575527228No Hit
CTGTAT1930680.6668009929832344No Hit
ATCAGC1887500.651887870727337No Hit
AGGTCA1845100.637244137896164No Hit
AGCCTA1844200.6369333039445588No Hit
CAAGGA1824390.6300915032986735No Hit
TTCCAT1778420.6142147957928004No Hit
CTCGTC1749580.6042542944991439No Hit
CTAGTT1710800.590860804895538No Hit
CAGTAG1693740.5849687746573349No Hit
CCATGA1651080.5702352453512537No Hit
TTCTAC1626770.561839274947343No Hit
GGTGGT1615570.5579711191051464No Hit
TGATAA1594930.5508426604816697No Hit
ATGTGA1570160.542287819391383No Hit
ATTGAA1558870.5383885801540258No Hit
TCGGAC1555080.537079623846711No Hit
TGGCAT1524270.5264387415700968No Hit
GGCGTT1521990.5256512955593641No Hit
CCGACT1464830.5059098859218675No Hit
AATCGG1461250.504673457536594No Hit
GTCTCA1446570.4996034104148577No Hit
CGACAA1445000.4990611778548354No Hit
CGAATT1431970.49456099297770845No Hit
TAAGTC1386280.47878098936789015No Hit
AACAAT1385240.4784218034682576No Hit
CGAAGC1381610.47716810653011704No Hit
TCCAGA1365030.47144185439943664No Hit
CTACCG1355120.4680192272212073No Hit
AAGCAT1354130.4676773098744418No Hit
GTACTT1349580.4661058715635494No Hit
ATGCCG1337190.4618267241631193No Hit
CCAGCG1336490.461584964422982No Hit
CAGTCC1295570.4474523807566707No Hit
ATACCT1283810.4433908171223642No Hit
GCGCAG1282800.44304199235445185No Hit
GGCCAG1280910.44238924105608113No Hit
AACCTT1257270.43422466926058745No Hit
GTCTGC1228250.4242020011766101No Hit
TGCATA1227860.42406730646424795No Hit
AGAACG1183840.4088640725201857No Hit
TGCGAC1169450.40389418300507773No Hit
ACCTGC1147840.39643071445598216No Hit
TACAGT1143040.3947729333807551No Hit
ACTAGC1132010.39096349061830604No Hit
CCTGCT1124470.3883593928459701No Hit
GCACAT1103360.3810686098255441No Hit
CCGGAA1097290.37897220750749644No Hit
CTCCGC1076360.3717435912773914No Hit
GGCCGT1074640.3711495530587684No Hit
CTTAGC1070100.3695815684584494No Hit
TTAGTA1056760.36497431855354734No Hit
CCACTT1054290.36412125204192003No Hit
CTATGC1053630.3638933071440763No Hit
GCCATA1029250.3554731607661518No Hit
AGGCTT1028450.3551968639202806No Hit
GAACCG1004550.3469424956498789No Hit
CGTTGC994210.3433713589169937No Hit
AGGTGT984980.3401835840577549No Hit
TCGTGG978150.3378246997361296No Hit
CACCTA966580.33382875660271755No Hit
GTCGAG957940.3308447506673087No Hit
GCATAA952770.32905918230086617No Hit
ATCCAC949690.327995439444262No Hit
AGTCAA946920.32703876161543305No Hit
TATTCT943010.32568836078123764No Hit
GACTTG934910.32289085521679184No Hit
ACGAAC933240.32231408555103574No Hit
TAATGA927720.32040763731452454No Hit
AACATA911700.31487479297595394No Hit
CTGGAG906910.3132204656113002No Hit
TGTGAT905750.312819835184787No Hit
AACTAG895470.3092694207153422No Hit
CGTCTC892030.3080813442780961No Hit
AGAGGT890160.3074355004008722No Hit
TACTCG870880.3007767464153765No Hit
CACATT865850.29903952999696143No Hit
GGACTA865620.2989600946537735No Hit
AGATGC865530.29892901125861293No Hit
TCAAGC842920.29112017165217846No Hit
CATAAC823620.28445451024553603No Hit
GTAAGA807240.2787973323263234No Hit
CTTCAC800740.27655242045362No Hit
TCTCGC787010.2718104758363557No Hit
GAGATA785900.27142711396270947No Hit
CTGTTC780520.26956901767422575No Hit
CAAGCT780030.2693997858561296No Hit
ACCGCT778630.26891626637585503No Hit
GTGGTG778000.2686986826097315No Hit
GCTGTC727460.2512436293718191No Hit
AAGCGC724240.25013153456718756No Hit
CCTCGG718540.2481629195403553No Hit
TACGGC711290.2456589793746477No Hit
CATACG696530.24056130256832425No Hit
ACAGCC693430.2394906522905734No Hit
GGATTC686620.23713867539009487No Hit
GAGTCG679780.23477633735789621No Hit
GTTACA673570.2326315830918211No Hit
GTTGGC672780.2323587399565233No Hit
ATTGCT660000.22794489784373106No Hit
CTGAGG654500.22604535702836664No Hit
TCCTCC653150.22557910610095902No Hit
CATGAG646670.22334110164940238No Hit
AATTGC642990.22207013615839488No Hit
TTAGCG637070.22002553949894813No Hit
GAGTGA619730.21403680536469008No Hit
GAATAC611620.2112358460896709No Hit
TATGGT586270.2024806897861276No Hit
AGTAGG558350.19283792986522308No Hit
ATTCGA548320.18937385816011307No Hit
CATTAA536830.18540554471128812No Hit
AGTTCC530900.1833574943412679No Hit
GACACG527980.18234901085383806No Hit
AGGACT511570.1766814718029053No Hit
GACTAA498050.17201205510768222No Hit
GACCGA490300.16933542941330507No Hit
GAGCAC480850.16607167292145164No Hit
ACGTAA476680.16463147561234806No Hit
GCTTCT475080.16407888192060568No Hit
TGTGTG467810.16156803433375125No Hit
AATGTA461880.15951998396373107No Hit
TCATGT458270.15827319444673732No Hit
GATTCA452360.15623205149786393No Hit
GGCAGG446540.154221991944151No Hit
AGTTAG439350.15173877404188368No Hit
TTCGTT436690.150820087029362No Hit
GCCTGG427820.1477566457507652No Hit
CCTGAC417110.14405772172666464No Hit
GCGGCT400550.138338377017131No Hit
TGACAC398930.13777887590424187No Hit
TGCGGA391260.13512987989445185No Hit
GCGTAT373670.12905480299585906No Hit
CGCTTG355730.12285884622719766No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)