FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24980815
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4335901.7356919700177917No Hit
CATGAG3797921.520334704852504No Hit
CGTACT3328841.3325586054738408No Hit
GCTGTC3246531.2996093201923156No Hit
TTACGT3215861.287331898498908No Hit
GTAAGA3178441.2723524032342421No Hit
CCTTAT3144641.2588220200181621No Hit
TCTTCA2986641.195573483090924No Hit
CGAATT2926361.1714429653315954No Hit
AACATA2864851.1468200697215043No Hit
CTGTTC2861161.1453429361692162No Hit
GAGAAG2784331.1145873343203574No Hit
CACATT2738531.0962532647553733No Hit
CATACG2723101.090076524725074No Hit
GAGATA2634411.054573279534715No Hit
TTGAAT2579001.03239225781865No Hit
GAAGAT2521701.0094546555026327No Hit
TCCAGA2505691.003045737298803No Hit
GACACG2463100.9859966538321508No Hit
GTCTCA2416620.9673903753740621No Hit
CCGGAA2375360.9508737004777467No Hit
TAGAGC2366040.9471428374134311No Hit
AACCTT2317330.9276438739088377No Hit
GCGCAG2278100.9119398226198785No Hit
AGTTAG2255650.9029529260754704No Hit
GGACTA2228800.8922046778697974No Hit
GAATAC2183010.8738746113767706No Hit
GGCCAG2164010.8662687746576723No Hit
GTGGTG2116310.8471741214207783No Hit
ATGGCC2104300.8423664319999167No Hit
CAGCCT2084460.8344243372363952No Hit
GCTCAA2060700.8249130382655651No Hit
TGCATA2044740.8185241354215225No Hit
ACCAGG2042720.8177155148861236No Hit
ATGCCG2031310.8131480097827073No Hit
CAGTAG2023900.8101817334622589No Hit
CGTGAA2010840.8049537214858682No Hit
TCGTGG2008070.8038448705536629No Hit
CTATTA2002400.8015751287538058No Hit
AAGGCG1986250.7951101675425721No Hit
TCCACG1943640.7780530779320051No Hit
TCATGT1883210.7538625140933153No Hit
TGTGAT1876170.7510443514352915No Hit
GTCTGC1861090.7450077189235018No Hit
CTTAGC1858700.7440509847256785No Hit
CCATGA1827040.7313772589084864No Hit
CTGTAT1823810.7300842666662396No Hit
CTGGAG1819280.7282708750695284No Hit
TTCTAC1808190.7238314682687494No Hit
AGGACT1787500.7155491123888472No Hit
CAGGAC1777870.7116941540938516No Hit
CCTCGG1769590.7083796105131077No Hit
GTTGGC1741880.6972870981190966No Hit
GCACAT1718980.6881200633366045No Hit
CGAGCC1718500.6879279158826483No Hit
TACGGC1702050.6813428625126923No Hit
AACTAG1683620.673965200895167No Hit
ACGAAC1667570.6675402704035076No Hit
CAGTCC1656830.6632409711212385No Hit
ACACGA1625240.6505952668077483No Hit
GGCCGT1603450.6418725730125298No Hit
AACTCT1569780.6283942297318963No Hit
GGTAAT1561220.6249676001363447No Hit
TATAAG1559200.6241589796009458No Hit
TCAATG1553260.6217811548582381No Hit
ACTAGC1540840.6168093394871224No Hit
ATTCAT1487640.5955129966736474No Hit
ATTGAA1478430.5918261674008634No Hit
CCAGCG1465370.5865981554244728No Hit
CAAGGA1431430.5730117292009889No Hit
ATCCGG1427020.5712463744677666No Hit
ATGTGA1411130.564885493127426No Hit
CGTTGC1395920.55879682068019No Hit
CCGTCG1357440.5433929997880373No Hit
ACTAAT1350060.5404387326834613No Hit
GAGCAC1334230.5341018697748653No Hit
ACAGCC1316580.5270364477700187No Hit
TGGCAT1298500.5197988936710032No Hit
CCACTT1295670.5186660243070532No Hit
CCTGCT1295600.5186380028033513No Hit
GCTTCT1284320.5141225376353814No Hit
GACTTG1282400.5133539478195568No Hit
TGATAA1206380.4829225947992489No Hit
TTAGTA1204300.4820899558321055No Hit
CAACGG1162670.4654251672733656No Hit
GACTAA1150570.4605814502048873No Hit
TAAGTC1148750.4598528911086368No Hit
TATTGG1136010.4547529774348836No Hit
GAGTCG1120220.4484321268141172No Hit
AGCCTA1119540.4481599179210126No Hit
CGAAGC1097380.4392891104633696No Hit
AGTGCA1087080.43516594634722683No Hit
CTACCG1072730.429421538088329No Hit
AAGCAT1061860.42507019887061337No Hit
AGTCAA1060250.42442570428546866No Hit
GCCATA1056490.4229205492294787No Hit
AAGCGC1055530.42253625432156633No Hit
GTCGAG1055140.422380134515227No Hit
CGAGAG1046510.4189254834159734No Hit
TCAAGC1033610.41376152059090143No Hit
AAGAGG1022930.40948623974037673No Hit
AACAAT1022110.40915798783986834No Hit
TGCGAC1015470.40649994806014134No Hit
GTACTT1012760.4054151155596805No Hit
TACAGT1002290.40122389922026164No Hit
GAGCCA996560.3989301389886599No Hit
CTGAGG984110.3939463144016718No Hit
GGAGGC962640.3853517189090908No Hit
TCTCTG947360.37923502495815287No Hit
TAATGA939560.3761126288313652No Hit
AGAACG937700.3753680574472851No Hit
GGCGTT935870.37463549527907714No Hit
GATCCT935370.3744353416812062No Hit
AGACGG933390.37364273343363696No Hit
GTACGC905150.36233805822588255No Hit
CCGACT878230.3515617885165076No Hit
CGTCAG870960.34865155520346314No Hit
ATCCAC857890.34341954015511506No Hit
ACCGCT854790.3421785878483148No Hit
TATTCT853510.34166619463776504No Hit
TTCACA852260.3411658106430875No Hit
TCCTCC840360.3364021550137576No Hit
TCGGAC819510.3280557499825366No Hit
AAGTTG812820.32537769484302254No Hit
AGATGC798940.3198214309661234No Hit
CATAAC786740.31493768317807086No Hit
TTCCAT781510.3128440765443401No Hit
TACGAA776870.3109866511560972No Hit
CCGCTC776030.31065039311167386No Hit
TACTCG763700.3057146053881749No Hit
ACCTGC762710.3053183012643903No Hit
GGATCG755220.30232000036828266No Hit
GAACCG752090.3010670388456101No Hit
AATCGG748240.2995258561420034No Hit
GCGTAT739090.29586304530096397No Hit
GACCGA732760.29332910075191704No Hit
CTTCAC731600.2928647444048563No Hit
TGCGGA706040.28263289248169043No Hit
GCATAA697460.279198256742224No Hit
CGCTTG692170.27708063167674873No Hit
GTTACA690270.2763200480048389No Hit
GGATTC687500.27521119707263353No Hit
CTATGC686400.27477085915731736No Hit
ACGCTA673210.2694908072454802No Hit
GACCAT663430.2655758028711233No Hit
CTCGTC659050.2638224573537733No Hit
CACCTA655630.2624534067443356No Hit
TATGGT646680.2588706573424446No Hit
ATACCT644140.25785387706525986No Hit
GGTGGT633850.25373471602107456No Hit
GAGTGA627670.25126081755138896No Hit
CAAGCT605460.24236999473395884No Hit
TGACAC604740.2420817735530246No Hit
AGTAGG594660.2380466770199451No Hit
TTAGCG580240.2322742472573453No Hit
AGGTCA574320.22990442865855257No Hit
TGTGTG564610.22601744578789762No Hit
GCGGCT558890.22372768862825332No Hit
TTCTGT547020.2189760422147956No Hit
CCTGAC531370.21271123460143312No Hit
AATTGC521720.20884827016252272No Hit
AGAGGT507890.2033120216454107No Hit
ACGTAA504740.20205105397882334No Hit
CTCCGC503230.20144659011325292No Hit
GGCAGG500550.2003737668286643No Hit
CGACAA494540.19796792058225482No Hit
ATCAGC494260.19785583456744704No Hit
AGGCTT493690.1976276594658741No Hit
CTAGTT488990.19574621564588662No Hit
TTCGTT483660.19361257829258172No Hit
ATTCGA483070.19337639704709392No Hit
CATTAA474880.19009788111396686No Hit
ATTGCT473320.1894734018886093No Hit
AGGTGT466080.18657517779143715No Hit
AATGTA445820.17846495400570397No Hit
AGTTCC435230.17422570080279606No Hit
GATTCA430760.17243632763782926No Hit
GCCTGG411460.16471039876000843No Hit
CGTCTC299330.11982395290145657No Hit
TCTCGC285040.11410356307430321No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)