FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24754786
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4323601.746571349879575No Hit
CATGAG3795771.533347935223516No Hit
CGTACT3328761.3446935069444752No Hit
GCTGTC3239011.3084378915656956No Hit
TTACGT3213291.298047981509515No Hit
GTAAGA3175531.2827943655016854No Hit
CCTTAT3127281.2633031850891379No Hit
TCTTCA2974961.2017716493287398No Hit
CGAATT2942401.1886186372202936No Hit
CTGTTC2868121.1586123184421793No Hit
AACATA2859241.1550251333216939No Hit
GAGAAG2814701.1370326529988988No Hit
CACATT2737461.1058306058472895No Hit
GAGATA2643471.0678621903659358No Hit
CATACG2615681.0566360783728852No Hit
TTGAAT2595591.0485204760000753No Hit
GAAGAT2534931.024016123589192No Hit
GACACG2473280.9991118485128492No Hit
TCCAGA2448590.9891380196136618No Hit
GTCTCA2370410.95755624791101No Hit
CCGGAA2361840.954094291099911No Hit
TAGAGC2323180.9384771090325726No Hit
AACCTT2311600.9337992257335611No Hit
GCGCAG2275890.9193737324168345No Hit
GGACTA2240030.9048876447568563No Hit
AGTTAG2229670.9007025954496234No Hit
GAATAC2181420.881211415037076No Hit
GGCCAG2162060.8733907051347566No Hit
ATGGCC2082110.8410939201817378No Hit
CAGCCT2072360.8371552878703941No Hit
GTGGTG2067690.8352687839838325No Hit
TGCATA2054780.8300536308413251No Hit
GCTCAA2051650.8287892288788115No Hit
ACCAGG2039370.8238285719779602No Hit
CAGTAG2026590.8186659339329373No Hit
ATGCCG2020800.8163269922834315No Hit
CTATTA2014230.8136729600490185No Hit
CGTGAA2006960.8107361542127652No Hit
AAGGCG2002390.8088900465550379No Hit
TCGTGG1961400.7923316323558603No Hit
TCCACG1938410.783044539346856No Hit
TCATGT1842730.7443934275982026No Hit
TGTGAT1838870.7428341331651989No Hit
GTCTGC1825280.7373442856666181No Hit
CTGGAG1822640.7362778252253929No Hit
CTTAGC1814850.7331309589992012No Hit
CCATGA1785190.7211494375269493No Hit
CTGTAT1784330.7208020299589745No Hit
CAGGAC1781930.7198325204669513No Hit
TTCTAC1771820.7157484617318041No Hit
AGGACT1753100.7081862876940241No Hit
CCTCGG1735270.7009836400928693No Hit
GTTGGC1726070.6972671870401141No Hit
CGAGCC1715880.6931508113218995No Hit
GCACAT1679910.6786202878102037No Hit
ACGAAC1669580.674447357371621No Hit
AACTAG1653330.6678829701860481No Hit
TACGGC1643960.6640978435442747No Hit
CAGTCC1622320.6553560996245332No Hit
ACACGA1619210.6540997769077866No Hit
GGCCGT1537040.6209061956746466No Hit
TATAAG1532740.6191691578347719No Hit
GGTAAT1531440.6186440068599259No Hit
TCAATG1531080.6184985804361226No Hit
AACTCT1530980.6184581842072883No Hit
ACTAGC1504750.6078622533840528No Hit
CCAGCG1460140.5898414957010737No Hit
ATTCAT1455350.5879065163399111No Hit
ATTGAA1438770.581208821599185No Hit
ATCCGG1437750.5807967800650751No Hit
ATGTGA1411590.5702291266020235No Hit
CAAGGA1399430.5653169451757734No Hit
CGTTGC1369860.5533717803094723No Hit
CCGTCG1326920.5360256396480261No Hit
ACTAAT1326500.535855975486922No Hit
GAGCAC1303450.526544644740617No Hit
TGGCAT1291730.5218102067212377No Hit
ACAGCC1277600.5161022195869518No Hit
CCTGCT1273910.5146115987429662No Hit
GACTTG1267390.5119777646229703No Hit
CCACTT1258300.5083057474219329No Hit
GCTTCT1247540.5039591131993627No Hit
TGATAA1184070.47831962675823575No Hit
TTAGTA1175500.47485766994713674No Hit
GACTAA1158050.4678085280155522No Hit
TAAGTC1150170.46462530518340983No Hit
CAACGG1146360.46308620886482316No Hit
TATTGG1143570.46195915408034627No Hit
AGCCTA1123360.4537950762329353No Hit
GAGTCG1107630.4474407494373007No Hit
CTACCG1075890.43461898640529556No Hit
CGAAGC1075030.4342715788373206No Hit
AAGCAT1070960.4326274523237648No Hit
AGTGCA1063020.4294199917543218No Hit
CGAGAG1056970.42697601990984696No Hit
AGTCAA1055870.4265316613926697No Hit
TCAAGC1042150.4209892987966044No Hit
AAGCGC1034430.41787070993059683No Hit
GCCATA1033320.4174223117905362No Hit
GTCGAG1031920.41685676458685605No Hit
TGCGAC1024680.41393207761925316No Hit
AACAAT1019160.4117022057876001No Hit
AAGAGG1009540.40781608857374085No Hit
GAGCCA997320.40287966941019004No Hit
TACAGT986940.3986865408571902No Hit
GTACTT984440.3976766351363329No Hit
GGAGGC958380.38714937790211557No Hit
CTGAGG957740.3868908420375761No Hit
TCTCTG945400.38190594739942413No Hit
AGAACG943110.3809808737591187No Hit
TAATGA939140.3793771434743972No Hit
GATCCT936580.37834300001623933No Hit
GGCGTT934530.3775148773251363No Hit
AGACGG930870.37603637534980106No Hit
GTACGC915730.3699203863042888No Hit
CCGACT881220.3559796477335736No Hit
CGTCAG878080.35471120614817675No Hit
ACCGCT856540.3460098584572696No Hit
ATCCAC854830.3453190829442032No Hit
TATTCT854570.34521405274923406No Hit
TTCACA852770.34448692063021674No Hit
TCCTCC844490.3411421128827371No Hit
TCGGAC805820.32552089119251526No Hit
AAGTTG795700.3214327928344846No Hit
AGATGC777100.31391909427130577No Hit
CCGCTC772360.3120043130245602No Hit
TACGAA771470.311644786587935No Hit
TTCCAT770990.3114508846895303No Hit
CATAAC768160.3103076714135198No Hit
GGATCG766240.30953206381990134No Hit
AATCGG756280.30550859942800557No Hit
TACTCG746020.3013639463496069No Hit
GCGTAT744900.3009115085866628No Hit
ACCTGC740290.29904924243740183No Hit
GAACCG737580.29795450463599243No Hit
GACCGA718280.2901580324709735No Hit
CTTCAC713090.2880614681944736No Hit
TGCGGA706030.28520949443877236No Hit
GGATTC691760.2794449525841185No Hit
CTATGC685260.27681919770988933No Hit
GCATAA679270.274399463602715No Hit
GTTACA678410.2740520560347401No Hit
CGCTTG677500.273684450352348No Hit
ACGCTA668490.27004475013437806No Hit
CTCGTC659180.2662838612299052No Hit
GACCAT647900.2617271666173967No Hit
GGTGGT644220.2602405853962947No Hit
CACCTA640580.25877016266672637No Hit
ATACCT637290.257441126738078No Hit
TATGGT634810.25643930026298756No Hit
GAGTGA612190.24730167330067002No Hit
CAAGCT595290.24047471062767417No Hit
TGACAC594780.24026868986061928No Hit
AGTAGG587380.23727936892688145No Hit
AGGTCA575030.23229043466584604No Hit
TTAGCG571460.2308482892964617No Hit
TGTGTG556310.22472826062806603No Hit
GCGGCT553630.22364564169530693No Hit
TTCTGT537850.21727111678525515No Hit
AATTGC512980.20722457467416605No Hit
CCTGAC512570.2070589501359454No Hit
AGAGGT497770.2010803082684698No Hit
CGACAA494810.19988457989497466No Hit
ACGTAA489710.1978243722244256No Hit
CTCCGC488580.1973678948385981No Hit
ATCAGC486010.1963297117575567No Hit
AGGCTT485900.19628527590583897No Hit
GGCAGG485560.19614792872780237No Hit
CTAGTT481300.1944270493794614No Hit
TTCGTT478750.1933969455441869No Hit
ATTCGA472470.19086006237339317No Hit
ATTGCT465000.18784246407947133No Hit
AGGTGT460740.18612158473113039No Hit
CATTAA458360.18516015448487416No Hit
AATGTA438100.176975878523046No Hit
AGTTCC425300.17180516123225625No Hit
GATTCA421850.1704114913374731No Hit
GCCTGG397660.16063964358245714No Hit
CGTCTC294900.11912847883233571No Hit
TCTCGC281150.11357399736762015No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)