FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23248803
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4048141.741225128880829No Hit
CATGAG3539001.522228907871085No Hit
CGTACT3111711.3384388004836207No Hit
GCTGTC3031641.3039983176768284No Hit
TTACGT3010161.2947591323303829No Hit
GTAAGA2970161.2775539454654934No Hit
CCTTAT2941131.2650672810983No Hit
TCTTCA2797241.2031759226485768No Hit
CGAATT2748671.1822845244978848No Hit
CTGTTC2688721.1564982506841321No Hit
AACATA2687221.1558530561766986No Hit
GAGAAG2599181.1179844398870773No Hit
CACATT2562371.102151366674663No Hit
CATACG2467741.0614481958490507No Hit
GAGATA2465371.0604287885273063No Hit
TTGAAT2419011.0404879769508995No Hit
GAAGAT2358531.0144737344111867No Hit
TCCAGA2290670.9852851348949019No Hit
GACACG2290190.9850786726525232No Hit
CCGGAA2244970.9656282089017658No Hit
GTCTCA2194140.9437647176932077No Hit
AACCTT2178810.9371708298272389No Hit
TAGAGC2168080.9325555384507322No Hit
GCGCAG2126420.91463633633095No Hit
GGACTA2075190.8926007932537431No Hit
GAATAC2048260.8810174011969563No Hit
AGTTAG2047490.8806862013498071No Hit
GGCCAG1993420.8574290900051929No Hit
CAGCCT1967180.8461424874218255No Hit
ATGGCC1957920.8421594866626035No Hit
GCTCAA1914780.8236036926288205No Hit
TGCATA1913170.8229111838575087No Hit
ACCAGG1906900.8202142708164374No Hit
GTGGTG1906200.8199131800463018No Hit
CAGTAG1887680.8119471785278579No Hit
CTATTA1882520.8097277094222872No Hit
CGTGAA1880220.8087384111775561No Hit
ATGCCG1871830.8051296232326456No Hit
AAGGCG1836970.7901353028798945No Hit
TCGTGG1820040.78285320753933No Hit
TCCACG1810620.7788013860326486No Hit
TCATGT1732390.7451523418216413No Hit
CTTAGC1709190.7351733334400055No Hit
GTCTGC1693840.7285708429806043No Hit
TGTGAT1693140.7282697522104686No Hit
CTGGAG1689780.726824516513818No Hit
CAGGAC1686330.7253405691467213No Hit
CCATGA1681490.7232587415360696No Hit
TTCTAC1671960.7191596057655097No Hit
CTGTAT1666540.7168283029453173No Hit
AGGACT1634530.7030598521566895No Hit
CCTCGG1620180.6968874913689105No Hit
CGAGCC1619330.6965218811480316No Hit
GTTGGC1593440.6853858239497319No Hit
ACGAAC1570150.6753681038976501No Hit
GCACAT1565200.6732389620231201No Hit
AACTAG1539600.6622276424295909No Hit
CAGTCC1536110.6607264898756293No Hit
ACACGA1525030.6559606531140549No Hit
TACGGC1517940.6529110337422533No Hit
AACTCT1450480.6238944860946174No Hit
GGCCGT1434060.6168317568865804No Hit
ACTAGC1426140.6134251298873322No Hit
GGTAAT1425360.613089628743467No Hit
TATAAG1423080.6121089330921682No Hit
TCAATG1419750.6106766012856661No Hit
CCAGCG1360540.5852086234289137No Hit
ATTCAT1355670.5831138919281135No Hit
ATTGAA1337290.5752081085636968No Hit
ATCCGG1328440.57140146096984No Hit
ATGTGA1326070.5703820536480954No Hit
CAAGGA1317030.5664936814166304No Hit
CGTTGC1279750.5504584472585535No Hit
CCGTCG1235380.531373593728675No Hit
ACTAAT1234790.5311198172224179No Hit
GAGCAC1222840.5259797676465321No Hit
ACAGCC1221170.525261451094923No Hit
TGGCAT1200310.5162889461448833No Hit
CCTGCT1192360.5128694152554865No Hit
CCACTT1191530.5125124076280401No Hit
GACTTG1171490.5038926090087305No Hit
GCTTCT1162140.4998708965790626No Hit
TGATAA1095250.4710995228442514No Hit
GACTAA1090650.4691209263547891No Hit
TTAGTA1083620.46609711476328475No Hit
TAAGTC1083330.4659723771585143No Hit
CAACGG1066040.4585354351361659No Hit
TATTGG1064380.457821419881273No Hit
AGCCTA1053160.45299536496567155No Hit
CGAAGC1019440.4384913924385699No Hit
GAGTCG1012320.4354288691766195No Hit
AAGCAT1006650.4329900339385215No Hit
CTACCG997650.42911886689392137No Hit
AGTGCA988530.4251960842887266No Hit
AGTCAA986840.42446916514368505No Hit
TCAAGC985780.4240132276917654No Hit
CGAGAG981790.42229701030199274No Hit
AAGCGC974290.419071037764826No Hit
GCCATA967980.4163569195368897No Hit
AACAAT956250.4113114984887609No Hit
GTCGAG952450.4096770057365964No Hit
TGCGAC950480.4088296502835006No Hit
AAGAGG938960.4038745564664125No Hit
TACAGT929010.3995947662337713No Hit
GAGCCA927620.3989968859902163No Hit
GTACTT926490.39851083946128324No Hit
CTGAGG903130.38846301033218783No Hit
GATCCT882800.37971847410810783No Hit
TCTCTG882010.3793786716675263No Hit
GGAGGC878440.3778431087398349No Hit
TAATGA877840.3775850309368616No Hit
AGAACG874080.37596774337156197No Hit
AGACGG868140.3734127731221259No Hit
GGCGTT863420.37138256107206896No Hit
GTACGC854110.36737805382926597No Hit
CCGACT822140.35362680822750314No Hit
CGTCAG812550.34950186467664596No Hit
TATTCT811250.348942696103537No Hit
ATCCAC810680.3486975221907124No Hit
TTCACA807790.3474544474397241No Hit
ACCGCT806770.34701571517466945No Hit
TCCTCC805030.34626728954604674No Hit
TCGGAC760480.3271050126752762No Hit
AAGTTG740770.318627156847602No Hit
TTCCAT732060.31488072740777234No Hit
CCGCTC731150.31448930940659614No Hit
CATAAC727370.31286341924786404No Hit
AGATGC726350.3124246869828094No Hit
TACGAA714850.30747819575915375No Hit
AATCGG704570.30305646273487713No Hit
GGATCG701710.3018262918740376No Hit
ACCTGC695830.29929712940489883No Hit
GCGTAT693250.2981873948521135No Hit
TACTCG690490.29700023695843614No Hit
GAACCG683930.29417858631259425No Hit
CTTCAC674360.29006224535516945No Hit
GACCGA667440.2870857480275436No Hit
TGCGGA657480.28280165649818617No Hit
CTATGC653430.2810596313281161No Hit
GGATTC641330.2758550623014871No Hit
CGCTTG634090.27274092347894213No Hit
GCATAA633950.272680705324915No Hit
GTTACA631880.271790336904657No Hit
CTCGTC629640.27082684644022315No Hit
ACGCTA628290.2702461713835332No Hit
GACCAT610360.2625339463713465No Hit
CACCTA603280.2594886282962611No Hit
ATACCT597170.25686053600264924No Hit
GGTGGT593530.2552948639979443No Hit
TATGGT593500.25528196010779564No Hit
GAGTGA571740.24592233845329584No Hit
CAAGCT557930.23998224768819282No Hit
TGACAC557340.2397284711819357No Hit
AGTAGG547330.23542287316899715No Hit
AGGTCA543150.23362493114161617No Hit
TTAGCG527120.22672995250551178No Hit
GCGGCT517210.22246736745973544No Hit
TTCTGT508430.2186908289428922No Hit
TGTGTG504820.217138060828336No Hit
CCTGAC487920.20986886937792026No Hit
AATTGC478840.20596329195959034No Hit
AGAGGT467390.20103830721951577No Hit
CGACAA462810.19906831332348593No Hit
CTCCGC461990.1987156069927557No Hit
ACGTAA458470.19720155054864544No Hit
ATCAGC454150.1953433903672374No Hit
GGCAGG453110.19489605550875028No Hit
CTAGTT452480.19462507381562827No Hit
AGGCTT447600.19252604101811177No Hit
TTCGTT446450.1920313918957462No Hit
ATTCGA442000.19011731485702726No Hit
CATTAA439730.1891409205024448No Hit
ATTGCT437570.18821184041174077No Hit
AGGTGT424770.18270618061497618No Hit
AATGTA410710.17665855743196757No Hit
AGTTCC395870.17027543310509363No Hit
GATTCA393890.1694237763552816No Hit
GCCTGG372230.16010716766794403No Hit
CGTCTC274850.1182211402453709No Hit
TCTCGC267540.11507689234581239No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)