FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22866185
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAG6440892.8167750763846264No Hit
TTACGT6339622.772486971482125No Hit
TTGAAT6329242.767947517261843No Hit
AAGGCG5600652.4493154411197144No Hit
CGTGAA4400351.9243918476125337No Hit
CCAGCG4007711.7526797758349284No Hit
GTACGC3674971.6071635911281221No Hit
ACGCTA3664091.6024054734097533No Hit
CAGCCT3493801.5279330592313498No Hit
CGTCAG3104041.3574804892027246No Hit
GGAGGC3035131.327344285896401No Hit
GTTGGC3031381.3257043096607501No Hit
AAGCGC2800151.2245811883355269No Hit
CTCGTC2790131.2201991718338674No Hit
CGTACT2545261.113110910280836No Hit
CCGGAA2482441.0856380283812102No Hit
ACCAGG2473291.0816364863662216No Hit
AGTTAG2344991.0255274327571477No Hit
GTCGAG2324911.0167459066739817No Hit
CAGTCC2219070.9704592173989671No Hit
CCACTT2213770.9681413843192469No Hit
GCTCAA2203820.9637899807073195No Hit
GTCTCA2167910.9480855682747252No Hit
CCGTCG2166610.9475170431796995No Hit
CTTAGC2153670.9418580318492131No Hit
TCTTCA2146770.9388404755756153No Hit
GTAAGA2092950.9153035366415516No Hit
TGTGAT2055540.8989431337146971No Hit
TCTCTG2052370.8975568071368267No Hit
GCGCAG2050070.8965509550456274No Hit
TTCTAC2043610.8937258226503458No Hit
GACTTG1976970.8645823516253367No Hit
AACATA1938590.8477977415121937No Hit
GGTACC1914450.8372406678245627No Hit
ATTCAT1834890.8024469320089905No Hit
TCATGT1818200.7951479444428531No Hit
GGTAAT1720050.7522242997684135No Hit
TCCAGA1697670.7424369215940482No Hit
GCCATA1686990.7377662692749141No Hit
CTGAGG1682420.7357676849024006No Hit
TGATAA1649490.7213665069184038No Hit
CATGAG1644360.7191230194280331No Hit
GACACG1631240.7133852892382354No Hit
GTACTT1564890.6843686430421165No Hit
GAGATA1555720.6803583544872046No Hit
CAAGGA1538410.6727882241834394No Hit
CTATTA1488530.6509743536142999No Hit
GTGGTG1446890.6327640574936308No Hit
GCTGTC1434470.6273324562011547No Hit
ATTGAA1399530.6120522509548488No Hit
CACATT1371870.5999557862406868No Hit
CTGTAT1363500.5962953592827137No Hit
GACTAA1362540.5958755253663871No Hit
ATTCGA1351300.5909599699293957No Hit
GCACAT1341770.5867922436558612No Hit
TACAGT1340190.5861012670019069No Hit
CGAATT1282070.5606838219842969No Hit
GGCCGT1279790.5596867164330211No Hit
AACTCT1261200.5515568075741537No Hit
AATTGC1257730.5500392828974312No Hit
TCCTCC1247860.5457228654451978No Hit
ATTGCT1223660.5351395521377965No Hit
ATCCGG1199760.5246874369292472No Hit
ACTAAT1175350.5140122849526495No Hit
GCGGCT1172390.5127177970439757No Hit
GGACTA1149140.5025499443829393No Hit
TTCACA1142230.4995280148393797No Hit
CGAAGC1124290.4916823685280251No Hit
CTGTTC1122030.4906940095166728No Hit
GATTCA1111810.4862245276157785No Hit
TCGGAC1109460.4851968091747705No Hit
TAGAGC1072370.46897635088669143No Hit
TACGGC1066280.4663130294799941No Hit
TTCCAT1059590.46338731187559273No Hit
CGCTTG1011410.4423168971999483No Hit
CGAGCC988970.4325032794058125No Hit
TACGAA968240.42343749077513365No Hit
TGCGAC967000.42289520529987845No Hit
ACACGA963680.42144327967258205No Hit
AGGTGT949400.4151982501672229No Hit
CTCCGC933940.408437174806379No Hit
CCGCTC933550.40826661727787117No Hit
AACCTT931400.407326364236098No Hit
GTCTGC930940.40712519381785817No Hit
TGACAC928290.4059662772779981No Hit
CTGGAG925840.40489482613737277No Hit
TACTCG892530.3903274638948298No Hit
AACTAG881320.38542502826772373No Hit
GAATAC874480.3824337116138962No Hit
ATACCT874170.3822981402450824No Hit
GAAGAT869040.3800546527547118No Hit
GCGTAT860780.3764423317663178No Hit
AGTGCA854690.3737790103596205No Hit
CATACG847830.3707789471658696No Hit
ACAGCC822070.35951340374443747No Hit
AGAACG815490.3566357921096151No Hit
TGGCAT814700.356290303782638No Hit
TTAGTA808900.35375380720483107No Hit
CCTTAT799640.3497041592202635No Hit
ACGAAC790300.34561952507600197No Hit
ATCCAC787920.3445786868251088No Hit
TATAAG772400.33779137184449437No Hit
CTTCAC769330.33644877796624145No Hit
TGCATA755230.33028246732019356No Hit
CCTCGG748800.3274704547347973No Hit
GAACCG742470.3247021748490183No Hit
ATGTGA727950.31835218686457756No Hit
TCTCGC727820.31829533435507495No Hit
CAGTAG714080.3122864614276496No Hit
CGACAA713790.3121596365987593No Hit
TGCGGA702030.30701667112375763No Hit
TCAAGC700420.3063125746599181No Hit
TCGTGG698310.3053898146979918No Hit
CCTGCT698150.30531984237860404No Hit
AAGCAT689750.30164629561074574No Hit
CACCTA688540.3011171299453757No Hit
CAACGG683900.2990879326831301No Hit
TATTCT675240.2953006808962667No Hit
GGATCG674430.29494644602936604No Hit
CATAAC666890.29164900047821707No Hit
ACCTGC666170.29133412504097206No Hit
GAGTCG662260.28962417648593325No Hit
AGTCAA661630.2893486604783439No Hit
AAGAGG658320.2879011081210092No Hit
AGTTCC656060.2869127491096569No Hit
TATGGT646730.2828324882353571No Hit
CTACCG645050.2820977788817855No Hit
ATGCCG639830.2798149319617593No Hit
TAAGTC629660.2753673164106737No Hit
GATCCT629030.27509180040308434No Hit
TAATGA615690.2692578582741284No Hit
CGTCTC613090.268120808084077No Hit
CCTGAC612920.2680464624947275No Hit
AGAGGT612300.26777531975709984No Hit
CAGGAC611070.2672374075518063No Hit
AGATGC607930.26586420078382117No Hit
CTAGTT596580.2609005393772507No Hit
GAGCAC594810.26012647059402344No Hit
AGTAGG574530.25125747911162266No Hit
AATCGG571810.2500679496820305No Hit
GGATTC560810.24525735272412078No Hit
CGAGAG559860.24484189207775586No Hit
AGGACT557230.24369172207781928No Hit
AGGCTT550950.240945308541849No Hit
CAAGCT549300.24022371899816258No Hit
GTTACA548410.23983449797156808No Hit
ATGGCC547680.23951524926436132No Hit
GAGCCA540750.23648457318087823No Hit
TTCTGT537750.23517259219235742No Hit
ACGTAA528150.23097425302909075No Hit
ATCAGC516330.22580504793431871No Hit
CCATGA515130.2252802555389104No Hit
TCCACG513400.22452367983553007No Hit
GGTGGT510180.22311548690785105No Hit
GCCTGG509160.22266941337175397No Hit
AGGTCA500870.21904397257347477No Hit
CTATGC497680.21764889945568097No Hit
AGACGG493300.21573340721244058No Hit
GCATAA473620.20712681192774396No Hit
TTAGCG463670.20277540831581659No Hit
GGCAGG463220.20257861116753845No Hit
ACCGCT462570.2022943486200256No Hit
GACCAT450210.1968889869473198No Hit
ACTAGC445190.1946936054265283No Hit
GAGTGA421040.18413215846893569No Hit
AGCCTA396750.17350948573187874No Hit
TATTGG361850.15824677356541986No Hit
TCAATG359630.15727590763391444No Hit
CCGACT330250.14442724048633385No Hit
AACAAT324410.14187325082868No Hit
GGCGTT318710.13938048695049043No Hit
GCTTCT274350.1199806614002292No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)