FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25878291
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGCG3559921.3756395273551874No Hit
GTAAGA3218991.243895897144058No Hit
GTCTCA2969061.1473168765278974No Hit
GGTACC2880641.113149241578588No Hit
GTTGGC2865731.1073876555449507No Hit
AAGCGC2697301.042302213851757No Hit
AGTTAG2691921.04022325121856No Hit
CAGTCC2550430.9855480796626022No Hit
CCGTCG2480910.9586838636291709No Hit
GCACAT2433230.9402591538985322No Hit
GAGATA2405610.9295861152500372No Hit
ATTCAT2404190.9290373927706432No Hit
ACCAGG2374590.9175992340452466No Hit
CTATTA2321370.8970337337964087No Hit
GACTTG2269460.8769744493560259No Hit
CGTACT2259090.8729672295593245No Hit
GTCGAG2258000.8725460270927474No Hit
GAGCAC2257020.8721673312971092No Hit
CCGGAA2193460.8476062039800077No Hit
TGATAA2192700.8473125215262476No Hit
CCACTT2174470.8402680068788159No Hit
GTACTT2170940.8389039291659562No Hit
CACATT2144880.8288337123962322No Hit
CAAGGA2141230.8274232637696206No Hit
AGGACT2133530.8244477968038925No Hit
GAGAAG2127070.8219514959469311No Hit
TTGAAT2109900.8153165910376384No Hit
TTACGT2100330.8116185106659478No Hit
CTTAGC2096160.8100071214130793No Hit
TGTGAT2020700.7808475451489436No Hit
TTCTAC2019350.780325872369238No Hit
CGAATT2016670.779290255295452No Hit
GAATAC1997400.7718438593955064No Hit
GCCATA1946970.7523564828913933No Hit
GCTCAA1941150.7501074935744405No Hit
TCTTCA1914690.739882707092211No Hit
AACATA1896640.7329077488154068No Hit
TACGGC1882940.7276137361620981No Hit
TACAGT1871560.7232162278413208No Hit
ATTGAA1854650.7166817932451567No Hit
AAGGCG1849860.7148308209379051No Hit
ATCCGG1845450.7131266898575335No Hit
CCTTAT1840350.7111559260230902No Hit
CTGTAT1825710.7054986745453941No Hit
GCGCAG1809120.6990878957192344No Hit
TCATGT1803420.6968852773160329No Hit
GGACTA1776950.6866566265909909No Hit
AACTCT1742540.6733597670727174No Hit
CTGTTC1740360.6725173621395633No Hit
ACTAGC1731950.6692675339341381No Hit
GGTAAT1681620.6498187998581514No Hit
CTGAGG1656530.6401234146412528No Hit
TCCAGA1655160.6395940133759219No Hit
CAGTAG1638580.6331870987925748No Hit
CATGAG1599570.6181126875804898No Hit
AACTAG1597560.6173359747751503No Hit
GACACG1579730.610446029840224No Hit
ACTAAT1548320.5983084431657407No Hit
CGAGCC1518000.5865920589578345No Hit
TCGGAC1496720.5783689502525495No Hit
TAGAGC1494890.5776617938178374No Hit
TTAGTA1493460.5771092070956308No Hit
ACAGCC1493240.5770241937537529No Hit
ACACGA1482780.5729821957717378No Hit
TTCCAT1471710.568704478978152No Hit
CGTGAA1470030.5680552861856295No Hit
CTGGAG1446460.5589472658762512No Hit
ATGCCG1444240.5580894039718465No Hit
AACCTT1442530.5574286184508861No Hit
GTGGTG1431860.5533054713698057No Hit
TCAATG1419260.548436525425887No Hit
ATTCGA1377940.5324694741240834No Hit
CCTCGG1372920.5305296242321411No Hit
GCTGTC1368040.5286438737395758No Hit
GACTAA1363040.5267117523332588No Hit
TACGAA1348910.5212515772390071No Hit
GAAGAT1346810.5204400862483539No Hit
TGACAC1334430.515656153646313No Hit
CGAAGC1313810.5076880849666618No Hit
ATCCAC1305640.50453099858874No Hit
CACCTA1303100.5035494809143308No Hit
TGGCAT1294030.5000446126832718No Hit
AATTGC1290090.4985221010150941No Hit
TCGTGG1286780.49724303664411224No Hit
CATAAC1285340.49668658567909296No Hit
CCTGCT1270160.4908206650895146No Hit
ACCTGC1262450.4878413338809738No Hit
TCCTCC1254230.4846649262889888No Hit
ATGGCC1249670.4829028315664276No Hit
ATTGCT1249190.4827173479114212No Hit
GTACGC1247770.4821686254320272No Hit
CAACGG1247040.4818865357067049No Hit
TATGGT1245800.4814073695979383No Hit
GTCTGC1243340.48045676586603037No Hit
ACGCTA1242050.4799582785432005No Hit
CATACG1235520.4774349279865506No Hit
ACGAAC1230740.4755878199221115No Hit
ATACCT1220860.4717699480232292No Hit
GGCCGT1214640.46936638899377087No Hit
GGTGGT1213820.4690495210831349No Hit
AAGAGG1203400.4650229800723703No Hit
GAGTCG1197610.46278558348385523No Hit
TATTCT1193880.4613442209147428No Hit
AGTGCA1189940.45982170924656496No Hit
TGCATA1181940.45673031499645783No Hit
CAGCCT1164490.44998721128841157No Hit
AGACGG1161020.4486463190324276No Hit
AGTCAA1148150.44367303853256773No Hit
AGATGC1145990.4428383620850388No Hit
CTTCAC1141630.4411535522187303No Hit
GCGGCT1137170.4394300999242956No Hit
GATTCA1131610.43728158092047115No Hit
TCAAGC1117740.43192187613934785No Hit
AAGCAT1114180.43054620569805013No Hit
AGTAGG1098560.4245102584247159No Hit
TATAAG1092620.42221489819401137No Hit
GGATCG1083180.4185670529788849No Hit
TTCACA1081360.4178637607869855No Hit
CGTCAG1078270.4166697097578816No Hit
CAAGCT1075960.41577706966816314No Hit
GCTTCT1058900.40918467142980963No Hit
GTTACA1051950.4064990226750291No Hit
CGCTTG1043050.4030598465717848No Hit
AGGCTT1041510.40246475317863917No Hit
TAAGTC1032910.39914150435977397No Hit
TAATGA1022850.3952540760902642No Hit
GATCCT1020550.39436530024335836No Hit
GAACCG1018400.3935344880386421No Hit
AATCGG1017580.3932176201280061No Hit
CGACAA1016020.3926147982492353No Hit
GGAGGC1006130.3887930621075402No Hit
AGGTGT990280.38266823724951543No Hit
GGATTC979770.3786069180534371No Hit
ACGTAA975200.3768409590880634No Hit
AGTTCC969870.3747813176689295No Hit
CTCCGC969260.37454559885735883No Hit
AGCCTA964520.37271394776417033No Hit
CTCGTC954010.36865262856809206No Hit
TACTCG951920.3678450018202516No Hit
CCGCTC947220.36602880769831364No Hit
CAGGAC940210.36331997348665723No Hit
CCATGA937540.36228822065568395No Hit
TGCGAC924280.35716423468613134No Hit
CGAGAG919030.3551355072094985No Hit
GCATAA913350.35294061729192244No Hit
CCTGAC901550.34838081077301436No Hit
GCGTAT880120.34009973842553975No Hit
GAGCCA864840.33419517540783505No Hit
CTAGTT860980.3327035776821584No Hit
ACCGCT835670.32292317912338186No Hit
CATTAA830030.32074374617705625No Hit
CTATGC828830.3202800370395402No Hit
GACCGA815940.3152990280540551No Hit
AATGTA813290.31427500370870703No Hit
GAGTGA804760.31097880458953026No Hit
CCGACT804360.3108242348770249No Hit
AACAAT794080.3068517932656372No Hit
AGAACG792420.30621032895873995No Hit
TCCACG788900.30485011548869284No Hit
TTCTGT786120.3037758559867806No Hit
TCTCGC769760.2974539547453114No Hit
GGCGTT757530.29272798578546005No Hit
ATCAGC754700.2916344050694847No Hit
TGCGGA740160.28601579601991495No Hit
TCTCTG733280.28335719696482276No Hit
TGTGTG727700.281200949475373No Hit
TTAGCG726700.2808145251941096No Hit
ATGTGA714990.27628949686051524No Hit
GGCAGG703720.2719344952106768No Hit
GACCAT645420.2494059596130208No Hit
AGAGGT645270.24934799597083132No Hit
CGTCTC638880.2468787448135582No Hit
CTACCG636470.24594746229571343No Hit
CGTTGC633390.24475727550942217No Hit
AAGTTG598350.23121696869395275No Hit
TATTGG595670.2301813516201669No Hit
TTCGTT534900.20669834804779033No Hit
GCCTGG534070.20637761589434173No Hit
AGGTCA511610.19769852653716583No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)