FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24131252
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4800201.9892047043394183No Hit
GAAGAT3570811.4797450211037537No Hit
ACTAGC3392611.4058988733779747No Hit
AGTTAG3183081.3190695617450765No Hit
ACACGA2997961.2423557633893179No Hit
GCACAT2915291.2080972839701811No Hit
ACAGCC2885321.1956777045799365No Hit
CGAGCC2874771.1913057805703575No Hit
CTGTAT2777471.150984623591018No Hit
CCACTT2634531.0917502332659739No Hit
GCTGTC2583861.0707525660085933No Hit
CAGGAC2533081.0497093147094068No Hit
TTACGT2520151.044351117795297No Hit
CTATTA2498851.0355243897001283No Hit
AACATA2490131.0319108183860497No Hit
TGTGAT2413161.000014421133226No Hit
CTTAGC2410510.9989162601260805No Hit
GTAAGA2368550.9815280201789779No Hit
TCATGT2354870.9758590229798272No Hit
CAAGGA2337300.968578008302263No Hit
GTCTGC2310310.9573933420445818No Hit
TCTTCA2249660.9322599589942536No Hit
TCCACG2228030.9232964787736666No Hit
TTGAAT2221500.9205904442919083No Hit
GCCATA2208520.915211527358796No Hit
GTCTCA2195540.9098326104256836No Hit
ACTAAT2184660.9053239342906866No Hit
GAGATA2155250.8931364191132727No Hit
CGTACT2103880.8718486715898538No Hit
CATGAG2074630.8597274604732484No Hit
ACGAAC2070240.8579082428048076No Hit
AAGCGC2002760.8299445051587047No Hit
GGACTA1979450.8202848322996253No Hit
GCGCAG1964740.8141890027090182No Hit
CCGTCG1905320.7895653321261573No Hit
AACTCT1900900.7877336824463149No Hit
CCAGCG1885160.7812110204642511No Hit
AACTAG1870670.7752063589572559No Hit
CATACG1851250.7671587035765902No Hit
ATCCGG1819070.7538232993464243No Hit
TGATAA1817630.7532265627991452No Hit
GAATAC1814750.7520330897045872No Hit
CGAATT1789200.7414451599941851No Hit
CCTTAT1730220.7170038255785485No Hit
CCTGCT1726810.7155907202825613No Hit
TGCATA1724870.7147867835452549No Hit
GAGAAG1722550.7138253746635276No Hit
CTGTTC1709410.7083801536696065No Hit
CAGTCC1699380.7042237178576561No Hit
TCGTGG1645400.6818543853422939No Hit
ACCAGG1637130.6784272941992401No Hit
GGATCG1632420.6764754684091816No Hit
TAGAGC1627490.674432474535511No Hit
TCCAGA1621350.6718880562019741No Hit
GGCCGT1613600.6686764532565488No Hit
TACGGC1609550.6669981317173266No Hit
GACTTG1605420.6652866581476999No Hit
GTGGTG1602230.6639647209353249No Hit
TATTGG1588600.658316443755177No Hit
GATCCT1570090.6506458927203611No Hit
ATTGAA1566650.6492203554129723No Hit
CACATT1538540.6375715607296298No Hit
TTAGTA1532220.6349525503276829No Hit
CGAGAG1520330.6300253298088304No Hit
AGGACT1504940.623647707959786No Hit
CCTCGG1486520.6160144529591751No Hit
TTCTAC1463610.6065205402521179No Hit
CAACGG1462960.6062511800050822No Hit
GGTAAT1461190.6055176913323851No Hit
GTACTT1442030.5975777800505336No Hit
ATTCAT1425640.5907857578214342No Hit
CAGTAG1398570.5795679395333487No Hit
ATGCCG1386220.574450094839671No Hit
TCAATG1370410.5678984248310034No Hit
CCATGA1361640.564264133497922No Hit
AACCTT1344820.5572939191053992No Hit
TACAGT1339040.5548986849086819No Hit
GCTCAA1313840.5444557953312991No Hit
GAGCAC1301320.539267502573012No Hit
GACACG1298900.5382646536532791No Hit
GAGTCG1291370.5351442187914659No Hit
CTGGAG1268140.5255176979627911No Hit
CGTGAA1249040.517602650703743No Hit
GGCCAG1231320.5102594759691705No Hit
ATGGCC1229830.5096420194028889No Hit
GAGCCA1190990.4935467086415574No Hit
GCTTCT1188750.4926184517902345No Hit
CGAAGC1183340.49037654573413764No Hit
AGACGG1175300.4870447666784964No Hit
GTCGAG1153390.4779652543514941No Hit
AAGGCG1125090.46623772359594107No Hit
GTTGGC1121410.46471273019733905No Hit
CCGGAA1111210.4604858463207794No Hit
CGTTGC1085190.4497031484317515No Hit
CGTCAG1058570.43867181031469066No Hit
TGTGTG1051980.43594091181012906No Hit
CATAAC1040740.43128305153831226No Hit
CTGAGG1014970.42060395374429804No Hit
CAGCCT1011470.419153552414106No Hit
AAGAGG1004010.4160621255788966No Hit
ACCTGC975830.4043843228689502No Hit
TCTCTG970560.402200432866061No Hit
AATCGG954920.3957192109220027No Hit
AGCCTA915300.3793006678642285No Hit
GTACGC906490.37564980051594504No Hit
ATTCGA896470.3714975087077952No Hit
CCGACT882350.3656461753414203No Hit
GCATAA880450.3648588146193161No Hit
TCAAGC861910.3571758315730986No Hit
AGTTCC842360.3490743041430258No Hit
GGCGTT835280.34614034945223726No Hit
CCTGAC828070.3431525227120416No Hit
CAAGCT816840.33849880644402536No Hit
ACGCTA813460.33709813315943987No Hit
AGTGCA802640.33261432104724614No Hit
TACTCG787240.32623255519440103No Hit
TTCTGT783260.3245832416817826No Hit
ATCCAC780900.3236052567848531No Hit
AGATGC774290.3208660702726904No Hit
ATGTGA761640.3156239054649962No Hit
CGTCTC760240.31504374493291937No Hit
GGATTC753510.31225483037515No Hit
AAGTTG745270.30884017124349783No Hit
GGTGGT744020.3083221707684292No Hit
AGTCAA741170.30714112968527285No Hit
TATGGT738860.3061838648073461No Hit
GACCGA735370.3047376074809546No Hit
AACAAT734740.30447653524152No Hit
GTTACA732990.30375133457642395No Hit
ACCGCT718560.2977715370922321No Hit
AAGCAT715300.2964205918532532No Hit
TATTCT706620.29282359655437684No Hit
CTCGTC694920.2879751121077348No Hit
GAGTGA690210.2860232863176764No Hit
CACCTA664750.2754726526414792No Hit
CTTCAC650310.26948871115348677No Hit
AGGCTT645930.2676736374888464No Hit
TTCGTT642610.2662978282270642No Hit
TGCGAC629470.26085260723314313No Hit
CTAGTT626170.2594850859789621No Hit
GCGGCT622030.25776946840553483No Hit
TTCCAT616560.2555026983266347No Hit
ATCAGC616250.25537423420881766No Hit
CATTAA614500.25464903354372165No Hit
GGAGGC606800.2514581506172991No Hit
AGTAGG589520.2442973120499508No Hit
AGAGGT582590.24142551741617052No Hit
TGCGGA580480.24055113261425473No Hit
AGGTGT578170.239593867736328No Hit
GCGTAT576050.23871533893061164No Hit
CCGCTC575820.23862002684319905No Hit
CTCCGC575610.23853300276338749No Hit
TTAGCG573440.23763375393866842No Hit
TAAGTC569390.23595543239944616No Hit
AATTGC559110.23169539649248203No Hit
GACTAA556640.2306718275537465No Hit
TGACAC546570.2264988157265939No Hit
TCCTCC545680.22612999938834505No Hit
CTATGC542420.22477905414936614No Hit
AGAACG539130.2234156768989856No Hit
ATTGCT534990.22170005932555842No Hit
TGGCAT531210.22013362588895097No Hit
TCGGAC528430.2189815928323984No Hit
TATAAG525200.21764307960482118No Hit
CTACCG520930.21587358998198686No Hit
ATACCT520660.21576170187937202No Hit
CGACAA516670.21410824436295306No Hit
AATGTA512820.21251280289974178No Hit
TTCACA509390.21109140959615358No Hit
GAACCG508570.21075160128450854No Hit
ACGTAA486940.20178812106392158No Hit
TACGAA476050.197275300925124No Hit
CGCTTG471170.1952530270704562No Hit
TAATGA467920.19390622583527783No Hit
GACCAT466350.19325561723859166No Hit
TCTCGC465790.19302355302576096No Hit
GGCAGG456360.18911575744184345No Hit
GATTCA444340.18413466487358385No Hit
AGGTCA418600.17346799909097133No Hit
GCCTGG326420.1352685720575128No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)