FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30189142
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC317045610.501974517858109No Hit
GAAGAT23248667.701000578287386No Hit
ACACGA19460486.446185188038799No Hit
CGAGCC18774786.219050544728963No Hit
CAGGAC16532595.476336492106997No Hit
CTATTA15943995.281365730765055No Hit
TCCACG14455074.78816854086148No Hit
GGATCG10463973.4661369309535193No Hit
TATTGG10120803.3524636109234245No Hit
GATCCT10071863.336252484419729No Hit
CGAGAG9623963.187887883663603No Hit
GAGCCA7546292.4996702456797215No Hit
AGTTAG3030821.0039437357974599No Hit
CATACG2566980.8502990909778092No Hit
ACGAAC2498380.8275756892991527No Hit
GCACAT2471560.8186917004795963No Hit
ATCCGG2207200.7311237927861614No Hit
GCCATA2140590.7090595685031393No Hit
TGCATA2080820.6892610594895343No Hit
CTGTTC2058830.6819769836453119No Hit
ACAGCC2013400.6669285268193446No Hit
CCGTCG1837020.6085035474012478No Hit
GCTGTC1629010.5396012910867092No Hit
CAGTCC1620490.5367790843476108No Hit
AACCTT1609470.5331287653024388No Hit
AACATA1538880.5097461862281478No Hit
GACTTG1533080.5078249656780573No Hit
CCTGCT1449130.4800169544401096No Hit
TCTTCA1426110.4723916963257849No Hit
ACTAGC1416490.46920512017201416No Hit
TCGTGG1383960.4584297228453859No Hit
CGTACT1330770.4408108054213664No Hit
AACTAG1296090.4293232315115149No Hit
CATGAG1290390.42743513545366746No Hit
TTAGTA1262510.41820002701633585No Hit
GCGCAG1246330.41284048417142827No Hit
TACGGC1208690.40037242529118583No Hit
CCAGCG1205050.3991666937735428No Hit
CGAAGC1149700.3808322873170758No Hit
CCATGA1138280.3770494703029321No Hit
CAAGGA1137180.3766851008882598No Hit
GTCTCA1077470.3569064665700006No Hit
CCTCGG1043510.3456573890042983No Hit
TCAAGC1031070.3415367021692766No Hit
ACCAGG1028910.3408212131368291No Hit
CAACGG1010890.3348521796346514No Hit
AACTCT923960.3060570585278641No Hit
GAGTCG903500.29927978741495864No Hit
CATAAC861960.2855198733372416No Hit
GCTCAA861410.2853376886299054No Hit
AAGCAT842380.279034097756074No Hit
AAGAGG832070.27561896260582697No Hit
ACCTGC809630.2681858265465113No Hit
GACACG808820.2679175181593435No Hit
TAGAGC787550.2608719386592703No Hit
TTACGT755470.2502456015477353No Hit
GTTGGC729120.2415172978417207No Hit
AGTGCA728550.24132848823593595No Hit
CCGGAA713850.23645918787622383No Hit
CTGTAT699060.23156007547349308No Hit
GTACTT694480.23004297372876648No Hit
CAAGCT666350.22072505406082757No Hit
TAAGTC665000.2202778734155479No Hit
TTGAAT658250.21804197018914945No Hit
CCACTT655230.2170416105234127No Hit
TGGCAT653230.21637912067855392No Hit
TACAGT650490.21547150959109734No Hit
CTATGC647280.214408213390099No Hit
GTAAGA643430.21313292043874582No Hit
AGATGC641750.21257642896906442No Hit
AGGACT619660.2052592286325991No Hit
GCATAA600950.19906163613394512No Hit
GAGATA600590.19894238796187053No Hit
GTCTGC599210.19848526996891794No Hit
TGTGAT588210.1948415758221946No Hit
TCATGT576450.19094613553442494No Hit
TCAATG565320.18725937954778574No Hit
ACTAAT551410.18265176267679287No Hit
GGACTA548950.18183690016761656No Hit
TTCCAT545120.18056823211471196No Hit
GAGCAC542680.17975999450398425No Hit
CACCTA536200.17761352740664177No Hit
AGGCTT534110.17692122551876432No Hit
TAATGA532190.17628523526769987No Hit
GAGAAG518420.17172399268584712No Hit
GCTTCT501880.16624520166886492No Hit
GTTACA495980.16429085662653148No Hit
TATGGT493830.16357868004330828No Hit
CGAATT480330.15910687359051146No Hit
ATGTGA473480.15683784587187008No Hit
TCGGAC472050.15636416563279604No Hit
GAGTGA469440.15549961638525533No Hit
CGACAA468830.1552975569825734No Hit
GAACCG464980.15402226403122024No Hit
TGATAA460530.15254822412640942No Hit
CGTTGC453720.15029244620466523No Hit
TGTGTG433370.14355161203322703No Hit
TACGAA421880.1397456078745133No Hit
CACATT419310.13889430842386974No Hit
GCGGCT403970.13381301131380283No Hit
GTGGTG399090.13219653609234736No Hit
AGTTCC398310.13193816505285244No Hit
ATTGAA394780.13076887047667668No Hit
CCTGAC393760.13043100065579868No Hit
AGTAGG390800.1294505156854077No Hit
TGCGAC388740.12876815114520312No Hit
CGTGAA371600.12309061317476329No Hit
ATTCAT365040.12091764648362648No Hit
CTTAGC358160.11863868141731221No Hit
TGCGGA357660.11847305895609753No Hit
TTCTAC357400.11838693527626588No Hit
CCGCTC353060.1169493323129223No Hit
GCGTAT353010.11693277006680083No Hit
CTGGAG349600.11580322488131661No Hit
NNNNNN348540.11545210526354144No Hit
AAGGCG337330.11173884968310793No Hit
GACTAA337160.11168253804629492No Hit
ACGTAA335090.11099686105686607No Hit
AGAACG334110.11067224103288525No Hit
TCCTCC332810.11024162263372705No Hit
CTACCG324470.10747903998066588No Hit
TTCACA313910.10398109359981149No Hit
CGTCAG305220.10110257522390002No Hit
CAGCCT304340.10081107969216217No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)