FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22745024
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGA3104371.3648567704303147No Hit
GAACCG2751201.2095832477468478No Hit
GATTCA2750991.2094909198600976No Hit
TAAGTC2718461.1951888905459058No Hit
TTCCAT2695551.1851163577580748No Hit
AGTGCA2588101.1378752557042806No Hit
CCGACT2537651.1156945800540812No Hit
TATAAG2423641.0655693306808556No Hit
ATCAGC2407101.0582974104577776No Hit
TTCTGT2358381.0368773407317573No Hit
ATACCT2266100.996305829354148No Hit
TACGAA2255890.9918169354316794No Hit
TAATGA2209560.9714476449881961No Hit
TGCGAC2195260.9651605555571189No Hit
AAGCAT2192110.9637756372558676No Hit
CGACAA2180990.9588866558241486No Hit
TACTCG2163070.9510080094881412No Hit
TGACAC2154640.9473017043200307No Hit
AATTGC2139000.940425475040167No Hit
GGCGTT2128770.935927787985627No Hit
AGAACG2071950.9109464997706752No Hit
TTCACA2063200.9070995044894216No Hit
AGCCTA2044360.8988163740781281No Hit
CGTCTC2006530.8821841647650054No Hit
AGTTCC1994900.8770709584654648No Hit
GACCAT1984620.8725512885807464No Hit
GGTGGT1971490.8667785973758481No Hit
ATTCGA1970980.8665543725080265No Hit
TCGGAC1933240.849961732289225No Hit
TCAAGC1927050.8472402579131155No Hit
CTAGTT1914580.8417577400665746No Hit
CTTCAC1861670.8184955091715885No Hit
TTACGT1819310.7998716554442854No Hit
ATTGCT1814140.797598630803819No Hit
CTCCGC1781450.7832262564330554No Hit
GGCAGG1766870.7768160631529779No Hit
TGGCAT1753900.7711137170046513No Hit
CCTGAC1753270.770836733344401No Hit
AACAAT1749470.769166038250828No Hit
AGGTGT1730930.761014804820606No Hit
AATCGG1711550.7524942598433838No Hit
AGGTCA1710840.7521821036548477No Hit
CTACCG1693240.7444441474319834No Hit
GCGTAT1681840.7394320621512643No Hit
TTAGCG1647620.724387013177036No Hit
GAGCCA1616520.7107136928059518No Hit
CTATGC1585420.6970403724348675No Hit
AGAGGT1548910.6809885098384596No Hit
AGGCTT1544400.6790056585563505No Hit
CATAAC1521910.6691177815420198No Hit
TCTCGC1513010.6652048377702305No Hit
TATTCT1468450.6456137395150693No Hit
ACCGCT1467750.645305979892569No Hit
CACCTA1463090.6432571801199242No Hit
AGTCAA1451930.6383506124240625No Hit
CGCTTG1415160.622184439110726No Hit
ACCTGC1387840.6101730207011433No Hit
GACTAA1381480.6073768047024263No Hit
GGATTC1366120.6006236792715629No Hit
TCAATG1363840.5996212622154191No Hit
CTATTA1356800.5965260797262734No Hit
TCCTCC1350010.5935408113880205No Hit
AGATGC1349580.5933517590484846No Hit
CAAGCT1332240.585728113542549No Hit
GTTACA1308500.5752906657737534No Hit
AGACGG1301710.5723053974355006No Hit
TATTGG1292710.568348488003354No Hit
ATCCAC1287310.5659743423440661No Hit
CGTCAG1273500.5599026846487389No Hit
TATGGT1242900.5464491925794407No Hit
GATCCT1230260.5408919331102926No Hit
GCATAA1228180.5399774473748632No Hit
ACTAGC1220270.5364997636406099No Hit
AAGTTG1183530.5203467800253805No Hit
GGATCG1174090.5161964216876623No Hit
GACCGA1167970.5135057232738027No Hit
CGAGAG1166950.5130572735381594No Hit
TGCGGA1156760.5085771727477624No Hit
GCCTGG1154560.5076099282199042No Hit
CCGCTC1147350.5044400041081514No Hit
AGTAGG1133690.49843429490335994No Hit
CTTAGC1118020.49154487592538926No Hit
GGAGGC1117500.4913162544915319No Hit
GTCTCA1108430.4873285690971353No Hit
GCACAT1069990.47042816925583375No Hit
ACGTAA1065490.4684497145397605No Hit
GAATAC1064520.46802324763429576No Hit
CATTAA1063500.46757479789865247No Hit
GTACGC1044490.45921692586475177No Hit
GCGGCT994200.43710659527112394No Hit
ACGCTA991490.4359151258754443No Hit
TAGAGC984230.4327232189335127No Hit
AACCTT974060.4282519112751871No Hit
AATGTA960690.42237370248543155No Hit
TTGAAT947840.41672411512953345No Hit
ACTAAT940030.41329039705563736No Hit
TACGGC929530.40867400271813303No Hit
GGTACC928030.40801451781277526No Hit
GAGTGA927590.4078210689072036No Hit
GTAAGA925270.4068010655869169No Hit
GACACG900060.39571732261087084No Hit
ATCCGG896460.39413455883801224No Hit
TGATAA887840.39034471891522293No Hit
GGCCGT887760.3903095463869372No Hit
GAGCAC884850.3890301456705431No Hit
TCTCTG869290.3821890889189653No Hit
GGTAAT867870.3815647765418933No Hit
GCTTCT859440.3778584713737827No Hit
CCACTT858260.3773396765815679No Hit
AAGCGC855940.37631967326128124No Hit
AAGGCG847040.37240672948949183No Hit
CATACG843560.37087672450906184No Hit
GGACTA836920.36795740466134486No Hit
GCGCAG830660.3652051543229851No Hit
TGTGTG829100.36451929002141303No Hit
TACAGT821170.36103281315508834No Hit
CTGTTC818470.35984574032544436No Hit
GAGATA817950.359617118891587No Hit
GCTGTC815980.35875099538255045No Hit
TCCAGA814600.35814426926962134No Hit
TTCGTT811580.3568165063268344No Hit
ACGAAC805350.35407744568658184No Hit
CAGTAG801660.35245511281940173No Hit
CGTACT800510.3519495077252941No Hit
TCTTCA790300.3474606138028256No Hit
CCTGCT790180.347407855010397No Hit
TCATGT789430.3470781125577181No Hit
CGAGCC788720.3467659563691821No Hit
CAGCCT777730.3419341302959276No Hit
CGTGAA760950.3345566924879921No Hit
CAGTCC759340.33384884535624143No Hit
GTACTT755830.3323056506777043No Hit
GAAGAT755450.33213858116834694No Hit
CATGAG753410.3312416816970604No Hit
TTCTAC753300.33119331947066755No Hit
TGTGAT749440.3294962449808802No Hit
CCAGCG747680.3287224493585938No Hit
AGGACT744920.3275089971327355No Hit
AACTAG742450.3264230453219131No Hit
CTCGTC740680.3256448531335909No Hit
TGCATA737480.324237952002161No Hit
GAGAAG734360.3228662233990169No Hit
ATGCCG723560.318117932080441No Hit
CGAATT722880.3178189655900121No Hit
GCTCAA718600.31593723532672463No Hit
ACACGA709170.31179127355504216No Hit
ACAGCC706590.31065695951782685No Hit
GTCTGC698900.3072760002363594No Hit
CAGGAC685100.30120873910706797No Hit
ACCAGG684940.30113839405049647No Hit
AACTCT680730.29928743974945904No Hit
CTGTAT679700.2988345934477801No Hit
CGTTGC676790.29755519273138603No Hit
CCATGA672340.2955987208454913No Hit
CCTCGG668260.2938049219029182No Hit
TCGTGG668120.2937433699784181No Hit
TTAGTA668120.2937433699784181No Hit
CCTTAT663540.2917297427340591No Hit
CCGTCG653250.28720567628330484No Hit
ATGGCC649130.28539429107658887No Hit
AACATA643470.2829058347003723No Hit
AGTTAG634800.2790940119474044No Hit
ATTGAA615640.27067019142296794No Hit
CACATT609450.26794871704685824No Hit
GTGGTG606000.2664319017645354No Hit
GGCCAG603430.2653019842933558No Hit
ATTCAT596670.2623299056532101No Hit
CTGGAG590280.2595204999563861No Hit
CAACGG588500.25873791120202816No Hit
GTTGGC565590.24866537841419734No Hit
AAGAGG558840.2456976963400874No Hit
CAAGGA554530.24380277637869274No Hit
GACTTG546750.24038224800290386No Hit
GCCATA542770.2386324147206879No Hit
TCCACG534790.23512395502418462No Hit
CTGAGG528910.2325387741951822No Hit
GTCGAG519430.22837082959332117No Hit
CCGGAA501040.220285544653635No Hit
GAGTCG492330.21645613563652427No Hit
CGAAGC475920.20924137077191038No Hit
NNNNNN455500.2002635829269734No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)