FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002208456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20699633
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCA6037622.9167763505758773No Hit
GAACCG4720692.280567003289382No Hit
AATTGC4663842.2531027482467927No Hit
TTCCAT4624392.2340444393386107No Hit
AGTGCA4458322.153815963790276No Hit
ATTCGA4374132.113143745108911No Hit
ATTGCT4045021.9541505880804746No Hit
TACGAA3872141.87063219913126No Hit
CGACAA3713901.7941863993434088No Hit
TCGGAC3344381.6156711570683404No Hit
CGCTTG3160711.5269401153150879No Hit
TGACAC2663771.2868682261178255No Hit
GCCTGG2562191.2377948923055786No Hit
AGTTCC2507731.2114852471055888No Hit
CTTCAC2310051.115985969413081No Hit
GGCAGG2194321.0600767656122212No Hit
CCTGAC2158051.042554715825155No Hit
ATGTGA2152911.040071580013037No Hit
TTAGCG2078981.004355970948857No Hit
CCACTT1896690.9162916076821265No Hit
CCGACT1875280.9059484291339851No Hit
TACTCG1859250.8982043304825743No Hit
TAAGTC1793480.8664308202952198No Hit
TCATGT1735440.8383916758330933No Hit
CGTCTC1684890.8139709530115823No Hit
TTCTAC1668320.8059659801697934No Hit
TGTGAT1662360.8030867020685826No Hit
TTACGT1602480.7741586529577601No Hit
TATAAG1591930.7690619442383351No Hit
GGCGTT1590670.7684532377941193No Hit
ATCAGC1544290.7460470434427509No Hit
AGCCTA1532730.7404624033672481No Hit
CTCCGC1521760.7351627924997511No Hit
TTCTGT1521710.7351386374821235No Hit
TGCGAC1516270.7325105715642398No Hit
GGTGGT1482820.7163508647713706No Hit
AGGTGT1479080.7145440694528256No Hit
TAATGA1478230.7141334341531562No Hit
AGAACG1458680.7046888222607618No Hit
ATACCT1456540.7036549875063003No Hit
AAGCAT1454850.702838547910487No Hit
TTCACA1437780.694592024892422No Hit
GCGTAT1381720.6675094191283487No Hit
GTCTCA1366770.6602870688576943No Hit
GTGGTG1359190.656625168185349No Hit
AGAGGT1312160.6339049586048217No Hit
AACAAT1305640.6307551443061816No Hit
CAGTCC1287820.6221462960237024No Hit
GACCAT1278380.6175858286956102No Hit
TCTCGC1276870.6168563471632564No Hit
TCAAGC1257230.6073682562391323No Hit
CTAGTT1233140.5957303687461512No Hit
TAGAGC1209980.5845417645810435No Hit
AGGTCA1200190.579812212129558No Hit
AGGCTT1195390.5774933304373078No Hit
CTACCG1193080.5763773686229123No Hit
CTGAGG1170440.5654399766411318No Hit
CATAAC1159930.5603625919358087No Hit
TGGCAT1139180.5503382596203518No Hit
CCGTCG1133370.5475314465720238No Hit
GAGCCA1130170.5459855254438569No Hit
AGTTAG1118220.5402124762308588No Hit
GACTAA1109990.5362365603293546No Hit
CACCTA1107430.5349998234268212No Hit
CGTCAG1092000.5275455849869415No Hit
TCCTCC1087780.5255069014991715No Hit
CTATGC1060000.512086373705273No Hit
ACCTGC1051530.5079945137191563No Hit
AGATGC1031090.49811994251299047No Hit
TACAGT1011400.4886076965712387No Hit
CAAGCT1000550.48336605774604796No Hit
AGACGG969760.46849139789096744No Hit
TCAATG962380.46492611728913263No Hit
GACTTG952260.46003714172130494No Hit
CTATTA944480.4562786209784492No Hit
GCCATA938190.4532399197608962No Hit
GTACTT937580.45294522854583946No Hit
CTTAGC929690.449133566764203No Hit
GGAGGC925900.4473026164280304No Hit
TGCGGA914120.44161169427496616No Hit
CCGCTC912350.4407566066509488No Hit
TATTGG906110.43774206045102343No Hit
AATCGG886020.42803657436825093No Hit
GTACGC870270.4204277438155546No Hit
GATCCT854450.41278509623817966No Hit
GCACAT851470.41134545718757426No Hit
AACTCT847110.409239139650447No Hit
ACTAGC841320.4064419886091701No Hit
ACGCTA837890.4047849543999162No Hit
TTGAAT835760.4037559506489801No Hit
GGATCG827220.3996302736381848No Hit
CGAGAG814640.3935528712030788No Hit
CGAAGC801930.38741266572214106No Hit
AAGTTG790560.3819198147136232No Hit
GCGGCT777240.3754849180176286No Hit
GAATAC775350.3745718583513051No Hit
ACCGCT755170.36482289323680284No Hit
TATTCT754870.3646779631310371No Hit
GACACG754760.3646248220922564No Hit
GGCCGT751580.36308856297114056No Hit
GACCGA749410.36204023520610246No Hit
AGTCAA747450.36109335851510027No Hit
AAGGCG738120.3565860322257887No Hit
GGTAAT734990.35507392812230054No Hit
TCTCTG732700.35396762831495615No Hit
GGATTC704770.34047463546817475No Hit
AAGCGC697590.33700597493685036No Hit
CATTAA688350.3325421276792685No Hit
GCTGTC682090.3295179194722921No Hit
TCCAGA680050.3285323947530857No Hit
CAAGGA677360.3272328548047205No Hit
CAGCCT669880.3236192641676304No Hit
CGTGAA659020.31837279433891413No Hit
GGTACC654230.3160587436501894No Hit
ATCCAC653220.3155708122941117No Hit
GAGAAG643060.3106625127121819No Hit
AATGTA638970.3086866322702436No Hit
AACCTT638460.30844025109044204No Hit
CATGAG628330.30354644451908885No Hit
CCTGCT627030.302918414060771No Hit
CTCGTC625370.30211646747553444No Hit
GAGCAC601980.2908167502293398No Hit
ACTAAT598360.2890679269531011No Hit
GCTTCT596620.2882273323396603No Hit
ATCCGG594890.2873915687297451No Hit
CAGTAG593670.2868021862996315No Hit
GCGCAG573610.2771111932274355No Hit
ACCAGG566690.2737681387877747No Hit
TGATAA566160.273512095600922No Hit
CGAGCC553900.26758928527863274No Hit
TTAGTA548570.2650143603995298No Hit
TCTTCA546240.2638887365780833No Hit
GAAGAT543790.26270514071433054No Hit
ATGCCG543420.26252639358388624No Hit
CGTACT542740.2621978853441508No Hit
CCATGA538700.2602461599198401No Hit
TGTGTG536000.25894178896794934No Hit
TCGTGG534560.25824612446027423No Hit
CTGTTC533660.2578113341429773No Hit
AACATA527850.2550045210946494No Hit
TTCGTT524930.2535938680651971No Hit
ACGAAC516230.24939089499799344No Hit
CATACG514760.24868073747974181No Hit
CCAGCG510930.24683046312946708No Hit
AGGACT510610.2466758710166504No Hit
ACACGA504740.2438400719471693No Hit
GTAAGA494240.23876751824537182No Hit
GCTCAA492580.2379655716601352No Hit
CCTTAT491670.23752595033931279No Hit
TGCATA482820.23325051221922632No Hit
CAGGAC473830.22890744004978253No Hit
ATGGCC470450.22727456085815628No Hit
CGTTGC465420.22484456608481898No Hit
GTTGGC462230.22330347596017766No Hit
GGCCAG448070.21646277496803928No Hit
AAGAGG446900.2158975475555533No Hit
GGACTA445560.21525019308313342No Hit
GTCGAG444670.21482023376936202No Hit
GTCTGC443330.21417287929694212No Hit
CTGTAT440920.21300860744729147No Hit
GAGATA434290.20980565210987073No Hit
ATTGAA401230.19383435445449684No Hit
TCCACG385660.18631248196525996No Hit
ATTCAT384120.18556850742232966No Hit
CGAATT380970.1840467413117904No Hit
CCGGAA339070.16380483653985556No Hit
GTTACA331400.1600994568357806No Hit
CACATT320520.1548433250000133No Hit
CTGGAG319200.15420563253464448No Hit
TATGGT310490.1499978284639153No Hit
GCATAA308390.14898331772355577No Hit
AGTAGG286590.13845173003791902No Hit
ACGTAA266990.128982963127897No Hit
TACGGC233170.11264450920458347No Hit
GAGTGA231920.11204063376389331No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)