FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002208460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20108523
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTGA2776331.380673259791383No Hit
GAACCG2489611.2380869544719917No Hit
GATTCA2465881.226285988284669No Hit
TTCCAT2459541.2231330963492446No Hit
TAAGTC2449491.2181352155998728No Hit
AGTGCA2328281.1578572926514792No Hit
CCGACT2274711.1312168477018425No Hit
TATAAG2219791.1039050456366188No Hit
ATCAGC2162171.0752505293402206No Hit
TTCTGT2132161.0603265093114993No Hit
ATACCT2046251.0176033316817947No Hit
TACGAA2037911.0134558366121669No Hit
TAATGA2001890.9955430341651648No Hit
AAGCAT1970310.9798382506760939No Hit
CGACAA1964160.9767798460384187No Hit
TACTCG1963900.9766505476309723No Hit
TGCGAC1953820.9716377478345873No Hit
TGACAC1929010.9592996959547949No Hit
AATTGC1901620.94567860603188No Hit
GGCGTT1895430.9426003093315208No Hit
AGAACG1888300.9390545491580857No Hit
TTCACA1869260.9295859273204701No Hit
AGCCTA1841770.9159151072408451No Hit
CGTCTC1800160.8952223890337445No Hit
AGTTCC1792940.8916318717192704No Hit
GACCAT1783990.8871810226937106No Hit
GGTGGT1776950.8836800196613148No Hit
ATTCGA1773400.8819145990981039No Hit
TCGGAC1747690.8691289758079198No Hit
CTAGTT1732770.861709236426763No Hit
TCAAGC1719210.8549658271768642No Hit
CTTCAC1669860.8304239948403968No Hit
ATTGCT1640470.8158083017832787No Hit
CTCCGC1612180.8017396404499724No Hit
AACAAT1590390.790903439302827No Hit
TTACGT1579540.7855077172997738No Hit
CCTGAC1569580.7805545936914412No Hit
AGGTGT1569320.7804252952839947No Hit
GGCAGG1561700.77663585734268No Hit
AGGTCA1545500.7685795719556329No Hit
CTACCG1543710.767689402150521No Hit
TGGCAT1519780.755788975649778No Hit
TTAGCG1503930.7479067458112164No Hit
AATCGG1493370.7426552412626228No Hit
GCGTAT1457100.7246181134238452No Hit
CTATGC1432820.7125436313746166No Hit
GAGCCA1395730.6940987162508157No Hit
AGAGGT1387330.6899213830871616No Hit
TCTCGC1358580.6756239630329886No Hit
CATAAC1335600.6641959730209921No Hit
AGGCTT1334470.6636340222501672No Hit
TATTCT1308640.6507887227719311No Hit
CACCTA1288450.6407482041321484No Hit
ACCGCT1272460.6327963520741926No Hit
CGCTTG1268900.6310259584953106No Hit
AGTCAA1263420.6283007459075935No Hit
ACCTGC1208890.6011828914535394No Hit
GGATTC1201290.5974033995435667No Hit
TCAATG1192560.5930619568627691No Hit
CTATTA1192510.593037091784414No Hit
GACTAA1185810.5897051712848328No Hit
TCCTCC1180430.5870296888538258No Hit
CAAGCT1170780.5822307287312947No Hit
AGATGC1168510.581101854173974No Hit
GTTACA1152730.5732544354451096No Hit
AGACGG1143790.5688085594352206No Hit
TATTGG1121090.5575198138620127No Hit
ATCCAC1118940.556450615492744No Hit
CGTCAG1104700.5493690411772162No Hit
TATGGT1093380.5437395874376253No Hit
GATCCT1065110.5296808721356611No Hit
ACTAGC1065100.52967589911999No Hit
GCATAA1054580.5244442866340805No Hit
AAGTTG1046010.5201824122040192No Hit
GCCTGG1024910.5096893491381739No Hit
GACCGA1019040.506770188939287No Hit
GGATCG1016940.5057258556483736No Hit
CGAGAG1010220.5023839891174503No Hit
CTTAGC1007880.5012203034504324No Hit
AGTAGG1005950.5002605114259262No Hit
GTCTCA994360.4944967862632178No Hit
TGCGGA991310.4929800164835577No Hit
CCGCTC985860.49026972294285365No Hit
GAATAC955040.4749428886447801No Hit
CATTAA937370.4661555699540936No Hit
ACGTAA931540.4632563018178908No Hit
GCACAT931070.46302257008135306No Hit
GGAGGC914200.45463309264434787No Hit
GTACGC898800.4469746485109821No Hit
TAGAGC885890.44055448527969954No Hit
AACCTT880760.43800332824046795No Hit
ACGCTA860440.42789816039696205No Hit
ACTAAT852640.424019208173569No Hit
AATGTA851750.4235766097788485No Hit
TTGAAT839090.4172807719393413No Hit
GCGGCT827680.41160656105871124No Hit
GTAAGA806070.40085987419364416No Hit
ATCCGG801780.398726450470778No Hit
GAGTGA801360.3985175838125953No Hit
TGATAA795790.395747614083839No Hit
GGTAAT792060.3938926792385497No Hit
GGTACC791000.39336553957742204No Hit
GGCCGT784750.39025740478303655No Hit
GACACG781110.38844722707878643No Hit
CCACTT779680.38773608583783103No Hit
GAGCAC769270.38255917652430266No Hit
TCTCTG764400.38013731789251753No Hit
GCTTCT752410.3741746721029685No Hit
AAGCGC751880.3739111022724046No Hit
TACGGC748390.3721755198032198No Hit
GCGCAG747280.3716235150637369No Hit
TACAGT745390.3706836151019148No Hit
CTGTTC740510.3682567834544586No Hit
TCCAGA737310.36666541843973327No Hit
AAGGCG732210.3641291804475147No Hit
TGTGTG724230.36016071394204335No Hit
GGACTA723910.3600015774405709No Hit
ACGAAC723300.3596982234846388No Hit
TTCGTT723290.35969325046896783No Hit
CAGTAG721930.35901692033770954No Hit
CGTACT718860.3574902045267074No Hit
TCATGT715910.35602316490375746No Hit
GAGATA714680.3554114839762224No Hit
TCTTCA713200.35467547765691193No Hit
GCTGTC705170.3506821460730855No Hit
CCTGCT694340.34529637010137443No Hit
TTCTAC693840.3450477193178236No Hit
CATACG692230.34424706379478986No Hit
CAGTCC690860.3435657606478606No Hit
CGAGCC679900.3381153354724263No Hit
GTACTT679790.3380606323000451No Hit
CAGCCT679400.3378666846888755No Hit
TGTGAT675750.3360515339689544No Hit
CCAGCG668750.33257042299924267No Hit
GAAGAT666970.3316852262098017No Hit
TGCATA662790.3296065056593167No Hit
CGTGAA658020.32723437718424175No Hit
ATGCCG655910.3261850708776572No Hit
AACTAG654920.3256927423262265No Hit
CATGAG654840.32565295820085843No Hit
AGGACT652640.32455889475323474No Hit
CTCGTC645550.3210330266424839No Hit
GCTCAA640370.3184570045248972No Hit
GAGAAG640060.3183028410390957No Hit
CGAATT632200.31439405072167653No Hit
CTGTAT624940.31078364134451847No Hit
ACACGA619840.3082474033522999No Hit
AACTCT618140.30740199068822704No Hit
GTCTGC617390.3070290145129008No Hit
ACAGCC616090.30638252247566866No Hit
CCTTAT609330.30302076388206134No Hit
TTAGTA599410.2980875323364128No Hit
CCGTCG596510.29664535779181794No Hit
ACCAGG594390.2955910784695624No Hit
CAGGAC590530.29367149442054996No Hit
AGTTAG589460.29313938174375115No Hit
CCATGA588270.2925475928789002No Hit
CGTTGC588200.29251278176920303No Hit
TCGTGG586170.2915032595879866No Hit
CCTCGG576470.2866794343871004No Hit
ATGGCC571550.2842327106769602No Hit
ATTGAA561960.27946358864845516No Hit
AACATA556230.2766140506689626No Hit
GTGGTG544370.27071605408313676No Hit
CACATT536390.2667475875776655No Hit
ATTCAT536140.26662326218589005No Hit
GGCCAG533240.26518108764129517No Hit
CTGGAG512070.2546532134657528No Hit
CAACGG510430.2538376388957061No Hit
GACTTG506720.25199265008175886No Hit
CAAGGA496970.24714395980251755No Hit
GCCATA491000.2441750694469206No Hit
AAGAGG482980.2401867108787652No Hit
CTGAGG476020.23672549197173756No Hit
GTTGGC474990.23621327135762282No Hit
TCCACG471560.23450752698246413No Hit
GTCGAG466860.23217020961708626No Hit
CCGGAA443030.22031951327305344No Hit
CGAAGC430360.21401870241787524No Hit
GAGTCG426760.21222841677630924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)