FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24026004
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAA3053881.2710727926291863No Hit
TTAGCG2825411.175979992344961No Hit
TCGGAC2730941.136660095453243No Hit
GATCCT2635241.0968282532542657No Hit
AATTGC2634431.0964911185397288No Hit
AGTGCA2397840.9980186467961962No Hit
AGAACG2383530.9920626001727129No Hit
CTACCG2373270.9877922271219135No Hit
CTATTA2335780.97218830064292No Hit
CGCTTG2329020.9693746825314771No Hit
TGCGAC2319180.9652791200734004No Hit
AGTAGG2266310.9432737961751776No Hit
GTACGC2254290.9382708834977302No Hit
AATCGG2200570.9159117762570921No Hit
TATTGG2198140.9149003721134817No Hit
ATGTGA2187630.9105259451384425No Hit
GGCGTT2123410.8837965730797348No Hit
GAGCCA2085440.867992863066201No Hit
TACGAA2076650.8643343270899315No Hit
CGTCAG2070870.8619286003615082No Hit
TGTGTG2053440.8546739607635128No Hit
GTTACA2041540.8497209939697005No Hit
GAACCG2026410.8434236504747107No Hit
AGTTCC1988000.827436805554515No Hit
TTCCAT1979630.8239530801709681No Hit
TACTCG1966240.818379951988687No Hit
TATAAG1950790.8119494194706702No Hit
TATGGT1940380.8076166140653269No Hit
TTCTGT1936940.8061848320677878No Hit
TATTCT1935760.8056936975453762No Hit
GCATAA1920240.7992340299285724No Hit
GGATCG1908170.7942103064662771No Hit
CTCCGC1890490.7868516129440417No Hit
CTAGTT1863280.7755263838297871No Hit
TAAGTC1843210.7671729347918197No Hit
CGAGAG1807170.7521725210734169No Hit
TCCTCC1793940.7466659874026492No Hit
AAGCAT1781390.7414424804058136No Hit
AGATGC1773790.7382792411089251No Hit
AGTCAA1759090.7321608703636278No Hit
CATAAC1754040.7300589810939847No Hit
ATTGCT1742520.7252641762650168No Hit
TGCGGA1730570.7202903986863567No Hit
ATCAGC1683210.7005784232783778No Hit
CGACAA1682480.7002745858195978No Hit
CTTCAC1681860.7000165320874832No Hit
CCGCTC1678870.6987720471535758No Hit
AGAGGT1670050.6951010247063973No Hit
ACCGCT1665220.6930907028900852No Hit
GGATTC1658010.6900897877150108No Hit
TCAAGC1657890.6900398418313757No Hit
AGGTCA1656500.6894613020126027No Hit
TAATGA1653270.688116925311425No Hit
GGTGGT1620150.6743318614281426No Hit
ATACCT1614800.6721051074494119No Hit
ACGCTA1604080.6676432751780113No Hit
GCGTAT1602930.6671646271265084No Hit
AACAAT1592430.6627943623084388No Hit
AGCCTA1585740.660009879295783No Hit
AATGTA1560960.6496960543251387No Hit
TTCACA1547010.6438898453525606No Hit
GACCAT1528540.6362023414297275No Hit
AGGTGT1527110.6356071529830761No Hit
GCACAT1521050.6330848858595046No Hit
GGAGGC1519130.6322857517213433No Hit
ATCCAC1487000.6189127413780502No Hit
CATTAA1486720.6187962009829017No Hit
CACCTA1482310.6169606897593124No Hit
ACCTGC1482280.6169482032884037No Hit
AAGTTG1476250.6144384226357409No Hit
TTCGTT1470830.612182533558223No Hit
TGGCAT1459980.6076665932462177No Hit
AGGCTT1458400.6070089724450225No Hit
ACGTAA1457500.6066343783177595No Hit
GGCAGG1457420.6066010810620026No Hit
TCTCTG1454820.6055189202499092No Hit
CCTGAC1437090.5981394159428259No Hit
TGACAC1430350.5953341221453222No Hit
CTGTTC1399920.5826686784868594No Hit
CCTGCT1397930.5818404092499111No Hit
AGACGG1393300.5799133305729908No Hit
GACCGA1365530.5683550206684391No Hit
GCGGCT1353870.563501945641897No Hit
ACTAAT1337980.5568882782172183No Hit
ATTCGA1325870.5518479061270447No Hit
TCTCGC1299690.5409513791806577No Hit
ACGAAC1281860.5335302533038786No Hit
AACCTT1271470.5292057722124744No Hit
CAAGCT1247630.5192831899969717No Hit
GATTCA1246460.5187962176315296No Hit
GCCTGG1241840.5168733011115789No Hit
CCGACT1236980.5148504928243581No Hit
ACTAGC1232320.5129109276765292No Hit
GGTACC1226420.510455255064471No Hit
CTGTAT1213060.5048946133530986No Hit
CTCGTC1204170.501194455807133No Hit
AAGAGG1160160.48287680298396685No Hit
CATGAG1159960.48279355984457506No Hit
CCATGA1152850.47983426623919645No Hit
ATTCAT1150040.47866470013074164No Hit
CTATGC1140880.4748521643465971No Hit
CACATT1103380.4592440757106342No Hit
GTCTGC1102750.45898185982155004No Hit
TTGAAT1086160.45207684140899995No Hit
CGTCTC1069320.4450677690722103No Hit
CATACG1066590.44393150021951217No Hit
GGACTA1058730.44066004484141436No Hit
GAGATA1026350.42718298057388154No Hit
CCTTAT1013540.42185125749583663No Hit
GAGTGA1007190.41920828782014685No Hit
GTCTCA1006950.4191083960528767No Hit
GTAAGA1003850.41781812739230373No Hit
ATCCGG995320.4142678074972434No Hit
CTTAGC984560.40978932659796447No Hit
TGATAA976900.40660111435925844No Hit
AACATA957810.39865555670431085No Hit
TGCATA939120.39087648532814695No Hit
TTAGTA935000.3891616766566758No Hit
CGTACT923630.38442930418225185No Hit
TACGGC919230.3825979551156322No Hit
ATTGAA910870.3791183918890549No Hit
AACTAG910620.3790143379648151No Hit
TCTTCA908000.3779238528387825No Hit
TCGTGG905690.3769623945788072No Hit
CTGGAG896540.37315402095163225No Hit
GCTGTC896320.37306245349830125No Hit
TCCAGA893930.3720676979825692No Hit
CAGTCC881390.36684835314270325No Hit
CGTGAA879670.3661324621439337No Hit
AAGGCG866300.36056765827559173No Hit
TAGAGC860130.35799960742535464No Hit
AAGCGC852090.3546532332218042No Hit
CGAATT836740.3482643222734833No Hit
CAGCCT835830.34788556598925063No Hit
ACAGCC825640.3436443280372383No Hit
GCTCAA822640.3423956809463613No Hit
GGTAAT806920.33585277019016563No Hit
CCGGAA802630.33406720485021146No Hit
GCGCAG796970.33171142400542347No Hit
TTCTAC788290.3280986717558192No Hit
CCACTT786250.32724959173402285No Hit
GAGCAC775940.3229584078983755No Hit
GGCCGT767110.31928322329422737No Hit
GACACG760460.3165153889094499No Hit
AGTTAG754860.31418458100647945No Hit
GAGTCG737670.30702983317575405No Hit
CCAGCG722000.3005077332044064No Hit
GCCATA720540.29990005828684624No Hit
ATGCCG716360.29816027667355754No Hit
TGTGAT712270.29645795447299517No Hit
GTACTT702190.2922625002476483No Hit
CAACGG693180.28851239681804763No Hit
TTACGT689400.28693910148354257No Hit
GACTTG684630.2849537526090481No Hit
GAATAC671850.27963451600191197No Hit
ACCAGG655240.2727211732754227No Hit
CAGTAG654350.27235074130512926No Hit
GGCCAG644500.26825101669008294No Hit
CGTTGC641640.2670606397967802No Hit
TCATGT623030.25931486567637296No Hit
GCTTCT622220.2589777309618362No Hit
GTGGTG617430.2569840577734025No Hit
CCGTCG609550.25370427808136553No Hit
CCTCGG603460.2511695244868851No Hit
CTGAGG580780.24172975247985476No Hit
AACTCT555920.2313826302534537No Hit
TACAGT546530.22747436485900857No Hit
AGGACT541650.22544323225784862No Hit
GAGAAG537570.22374507221425585No Hit
ATGGCC533920.22222588492035547No Hit
GAAGAT520150.21649459477322985No Hit
CAAGGA516340.21490881296781605No Hit
CAGGAC514420.21410967882965473No Hit
GTTGGC463840.19305748887746793No Hit
TCAATG441310.18368014922498138No Hit
CGAAGC438850.18265625861046225No Hit
ACACGA418520.17419459349128552No Hit
GTCGAG400570.16672352173087127No Hit
TCCACG390010.16232828397098412No Hit
CGAGCC383340.1595521252722675No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)