FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23229773
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTG4686452.017432542280977No Hit
GGCGTT4166061.7934139950485095No Hit
AATGTA3517081.514039762678697No Hit
AAGTTG3340911.4382017422210711No Hit
TTCGTT3335201.4357436897898228No Hit
CATTAA3323781.4308275849273258No Hit
TCGGAC3300321.4207284763393941No Hit
GTACGC3217511.3850802588557365No Hit
GGTGGT3138991.3512788093107928No Hit
AGTAGG3091701.330921313781241No Hit
AACAAT3054361.314847114519802No Hit
AGCCTA3051151.3134652671810438No Hit
GACCGA3047731.3119930186145168No Hit
AATTGC2941861.2664178853577261No Hit
CGTCAG2901871.2492029086982468No Hit
ACTAGC2896191.2467577707281083No Hit
TTAGCG2874221.2373000803753011No Hit
GTTACA2759201.187786036479995No Hit
AGTGCA2751701.1845574212025232No Hit
AGACGG2738521.1788836679549128No Hit
CGCTTG2614771.1256115158766296No Hit
GCATAA2600881.119632120382752No Hit
TATGGT2596061.11755719696443No Hit
GAACCG2405431.035494406251839No Hit
CCGACT2377121.0233074597844758No Hit
TACGAA2318500.9980726027757567No Hit
ACGCTA2253900.9702634631858004No Hit
TTCCAT2164890.931946257072766No Hit
GGAGGC2152930.926797691910291No Hit
CCTTAT2012830.8664871585271195No Hit
TCTCTG2010940.8656735474771965No Hit
ACGTAA1962330.84474781565881No Hit
CGACAA1955810.8419410727775946No Hit
AGTTCC1933350.8322724462266592No Hit
TACTCG1885370.8116179180915801No Hit
ATTGCT1876420.8077651038604639No Hit
CTCCGC1860120.8007482466574253No Hit
GAGCAC1815100.7813679453518552No Hit
CTCGTC1709380.7358573844006138No Hit
AGAGGT1631920.7025122458148859No Hit
TTGAAT1615920.6956245332229463No Hit
CTTCAC1613270.6944837558249062No Hit
GACTAA1585960.6827272913945392No Hit
CGTTGC1496340.6441474912389372No Hit
AGGTGT1483800.6387492464950045No Hit
GCTTCT1448790.6236780703797665No Hit
ATGCCG1428350.6148790175435636No Hit
ATTCGA1413730.6085853701626788No Hit
CCTGAC1400070.6027049855373102No Hit
GGCAGG1387520.5973024359730076No Hit
TGACAC1369010.5893342134682074No Hit
TACGGC1359400.5851972810926735No Hit
GCCTGG1354170.5829458600391834No Hit
AGAACG1346390.5795967097913527No Hit
CTACCG1337320.5756922377157969No Hit
GAATAC1335600.5749518086121633No Hit
GATTCA1326240.5709224967458787No Hit
TGCGAC1305220.561873764328218No Hit
GAGTGA1298360.5589206575544239No Hit
CGTGAA1288950.5548698215862893No Hit
GGCCAG1284630.5530101391864656No Hit
CAGTAG1283660.5525925716105792No Hit
AACTAG1281150.5515120616977187No Hit
GATCCT1279580.5508362048996346No Hit
AAGGCG1277000.5497255612441844No Hit
TCTCGC1255300.5403841010413661No Hit
AGGACT1241940.5346328610270966No Hit
CAGCCT1229900.5294498573016619No Hit
ATGTGA1227800.5285458450239698No Hit
TAAGTC1203760.5181970568545805No Hit
ACAGCC1172300.5046540919706792No Hit
AAGCAT1169900.5036209350818882No Hit
AATCGG1167400.5025447299893977No Hit
CTATTA1140800.49109390780529794No Hit
CAGTCC1127910.48554499434841664No Hit
TCAAGC1096320.4719460667997057No Hit
ATGGCC1078140.4641199033671143No Hit
TATAAG1076570.46344404656903015No Hit
TAATGA1075280.4628887247413051No Hit
TTCTGT1074870.46271222710613663No Hit
TATTGG1057630.4552907167883216No Hit
GTCTCA1053250.45340520546627816No Hit
GAGTCG1040800.4480457041056751No Hit
CTAGTT1036580.44622906990955097No Hit
CGTCTC1032200.4443435585875075No Hit
CTTAGC1028600.44279382325432104No Hit
TTACGT1027770.4424365231636142No Hit
GAGCCA1018710.4385363559084284No Hit
TATTCT1007070.4335255449977923No Hit
TCAATG1004130.4322599278090234No Hit
CAACGG981560.4225439482340184No Hit
TGGCAT963520.41477805228660647No Hit
CTGTTC955330.4112524044036074No Hit
ATCAGC930970.4007658619823793No Hit
TCCTCC930750.4006711559342401No Hit
TCCAGA925100.3982389324252114No Hit
AGGTCA923180.3974124069141786No Hit
GGATCG922580.3971541176919809No Hit
AGTCAA918840.39554411487361496No Hit
AGTTAG907950.3908561654907261No Hit
CCACTT904330.38929782051679973No Hit
GCCATA904170.3892289433908803No Hit
TTCTAC900450.38762755021325435No Hit
TAGAGC898160.386641746348533No Hit
AAGCGC896900.38609933898191773No Hit
ATACCT894420.38503174353016706No Hit
CGAGAG889640.3829740393933251No Hit
ACGAAC877860.37790296099750953No Hit
ACCGCT874690.37653833294023153No Hit
TGCGGA874290.376366140125433No Hit
CCGCTC872140.3754406037458911No Hit
GGATTC867510.37344747191459854No Hit
AACCTT858140.3694138552279439No Hit
CCTCGG855510.3682816874706438No Hit
TTCACA851780.3666759894726479No Hit
GACCAT850700.36621106887269195No Hit
GACTTG829140.3569298761550532No Hit
GGTAAT826140.35563843004406454No Hit
TGTGAT824520.35494104914413066No Hit
GCGTAT808900.3482169197262496No Hit
GGCCGT793350.34152292405095824No Hit
ATCCAC778200.3350011211904653No Hit
GAGAAG776500.33426930172757174No Hit
ACTAAT764650.32916808958916643No Hit
CATACG750110.3229088807712413No Hit
CTATGC743230.3199471643567072No Hit
CCGTCG727010.3129647457166284No Hit
GTACTT721660.31066166681869856No Hit
TCATGT716190.3083069300763292No Hit
GCGGCT700150.3014019982029097No Hit
GTGGTG696380.2997790809234339No Hit
CTGTAT681980.2935801395906882No Hit
ATCCGG673600.2899727001206598No Hit
CTGAGG658190.2833389719305479No Hit
ATTCAT657290.2829515380972513No Hit
GTCTGC635070.2733862272351951No Hit
TGCATA633920.2728911728926494No Hit
GGTACC623590.2684442934504784No Hit
AGATGC606140.2609323819048942No Hit
CATGAG605060.2604674613049383No Hit
CATAAC593360.2554308214720824No Hit
CACATT591490.2546258200628994No Hit
GGACTA563810.24271007727884383No Hit
AACTCT558810.24055766709386273No Hit
TACAGT556200.23943410897730252No Hit
TGATAA549850.23670054804237647No Hit
CGTACT547520.23569752489617526No Hit
GAGATA541910.23328252066862643No Hit
GCACAT537270.2312850840169639No Hit
GTAAGA533490.22965786191711818No Hit
TCTTCA532800.22936082931159077No Hit
CGAAGC531900.22897339547829418No Hit
CAAGGA522370.2248709016657201No Hit
ATTGAA519540.22365263750102077No Hit
AACATA501800.21601588616470768No Hit
CACCTA500710.21554666074438178No Hit
ACCTGC500050.21526254259996427No Hit
CCTGCT494730.21297237816314432No Hit
AGGCTT494350.21280879498908578No Hit
GCTCAA484500.20856854692467292No Hit
CTGGAG476980.2053313220064613No Hit
CCGGAA475950.20488792550835516No Hit
GCTGTC469620.202162974214169No Hit
GCGCAG460630.19829294070157294No Hit
CGAATT451320.19428515293713805No Hit
CAAGCT420100.18084550374211578No Hit
CCAGCG419370.18053125185510852No Hit
AAGAGG405810.17469391543343968No Hit
CCATGA403430.17366936818538864No Hit
GTCGAG400070.1722229485410813No Hit
GACACG394330.16975198164872296No Hit
ACCAGG342940.14762950976748673No Hit
TTAGTA329780.14196436616061636No Hit
TCGTGG318270.1370095179147898No Hit
GAAGAT259700.11179618500792064No Hit
CAGGAC257840.11099548841910767No Hit
GTTGGC243360.10476210852340226No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)