FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25324065
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAA3192971.2608441812165623No Hit
TTAGCG2933571.1584119690105044No Hit
TCGGAC2870401.133467316562329No Hit
GATCCT2757971.0890708107091023No Hit
AATTGC2744541.083767554695504No Hit
AGTGCA2493470.9846247038143362No Hit
CTACCG2491010.9836532957880182No Hit
AGAACG2483410.9806521978205316No Hit
CTATTA2460410.9715699276557693No Hit
TGCGAC2443690.9649675121272986No Hit
CGCTTG2423920.9571607085987182No Hit
AGTAGG2343470.9253925070876259No Hit
GTACGC2342220.9248989054482367No Hit
AATCGG2311660.9128313325684482No Hit
TATTGG2309330.9119112591126266No Hit
ATGTGA2288550.9037056254594198No Hit
GGCGTT2211690.8733550478566533No Hit
GAGCCA2190530.8649993593050721No Hit
TACGAA2179030.860458224222691No Hit
CGTCAG2150600.8492317485364218No Hit
TGTGTG2125950.8394979242076658No Hit
GTTACA2118220.8364454916696826No Hit
GAACCG2105200.831304136993804No Hit
TTCCAT2078670.8208279357994066No Hit
AGTTCC2072270.8183006954057336No Hit
TACTCG2049370.8092579133721226No Hit
TATAAG2047820.8086458473392798No Hit
TTCTGT2046500.8081246040080848No Hit
TATTCT2039730.8054512575291526No Hit
TATGGT2031000.8020039436796581No Hit
GCATAA2008660.7931822951804933No Hit
CTCCGC1998240.7890676319145445No Hit
GGATCG1980190.7819400242417637No Hit
CTAGTT1969910.7778806443594265No Hit
TAAGTC1951320.7705398007784295No Hit
TCCTCC1927320.761062649302156No Hit
CGAGAG1911900.7549735794786501No Hit
AAGCAT1875300.7405209234773327No Hit
CATAAC1868730.7379265532607028No Hit
AGATGC1862290.7353835176145694No Hit
AGTCAA1847400.7295037348861646No Hit
ATTGCT1820520.7188893252327381No Hit
TGCGGA1818500.7180916649834851No Hit
CCGCTC1792370.7077734163136921No Hit
ATCAGC1778620.7023437982804104No Hit
CGACAA1776560.7015303427786969No Hit
CTTCAC1775590.7011473079065308No Hit
ACCGCT1751760.6917372862532141No Hit
AGAGGT1739890.6870500450855738No Hit
GGATTC1735650.6853757483247654No Hit
AGGTCA1735400.6852770279968876No Hit
TAATGA1734760.6850243039575202No Hit
TCAAGC1734350.6848624026198006No Hit
ATACCT1704080.6729093453203504No Hit
ACGCTA1681820.6641192873261067No Hit
GGTGGT1680090.6634361426571919No Hit
AACAAT1675420.6615920469324337No Hit
GCGTAT1672860.6605811507749644No Hit
AGCCTA1660050.6555227211745034No Hit
TTCACA1626890.6424284568847853No Hit
AATGTA1623150.6409516007797327No Hit
GACCAT1607190.6346492950480107No Hit
GCACAT1591580.6284851977753176No Hit
AGGTGT1584840.6258236977357309No Hit
GGAGGC1579480.6237071339060296No Hit
CACCTA1570330.6200939699057004No Hit
ACCTGC1568550.6193910811712101No Hit
ATCCAC1567290.6188935307187058No Hit
CATTAA1561220.6164966011578314No Hit
TTCGTT1547790.6111933451442334No Hit
AAGTTG1540280.6082277864947827No Hit
ACGTAA1529140.6038288086845457No Hit
TGGCAT1528770.6036827025992865No Hit
AGGCTT1524500.6019965593991328No Hit
GGCAGG1519740.6001169243563386No Hit
CCTGAC1513710.5977357900479248No Hit
TGACAC1508380.595631072657569No Hit
TCTCTG1500630.5925707424933556No Hit
CTGTTC1489970.588361307712644No Hit
CCTGCT1487420.58735436036829No Hit
AGACGG1459780.5764398409181148No Hit
GACCGA1429370.5644315002350531No Hit
GCGGCT1412950.5579475491000359No Hit
ACTAAT1400760.5531339459127119No Hit
ATTCGA1387420.5478662292171498No Hit
TCTCGC1364800.5389340139507618No Hit
ACGAAC1351960.5338637379109554No Hit
AACCTT1330370.5253382503954243No Hit
CAAGCT1326050.523632363129695No Hit
CCGACT1305250.5154188318502578No Hit
GCCTGG1302380.5142855224862201No Hit
GATTCA1297420.5123269111811235No Hit
GGTACC1285290.5075370008724903No Hit
ACTAGC1281930.5062101996658119No Hit
CTCGTC1270020.5015071632457111No Hit
CTGTAT1265230.4996156817635715No Hit
CCATGA1215580.4800098246470304No Hit
AAGAGG1211960.4785803542993591No Hit
CATGAG1211940.4785724566731289No Hit
CTATGC1207860.47696134092216236No Hit
ATTCAT1202860.4749869343646054No Hit
CACATT1162780.4591600913992283No Hit
GTCTGC1150370.45425961432337186No Hit
CATACG1146440.452707730769132No Hit
CGTCTC1137880.4493275467425945No Hit
TTGAAT1131980.44699774700467715No Hit
GGACTA1100880.43471693821667257No Hit
GAGATA1067480.4215279024121917No Hit
CCTTAT1062310.41948636603167777No Hit
GAGTGA1059000.41817930889057503No Hit
GTAAGA1045050.41267071459499094No Hit
GTCTCA1043490.4120546997490332No Hit
ATCCGG1038750.4101829623324692No Hit
CTTAGC1027490.40573659876485074No Hit
TGATAA1020620.4030237641547674No Hit
AACATA1009710.398715609046178No Hit
TGCATA991460.3915090251110949No Hit
TTAGTA985320.3890844538584149No Hit
CGTACT979380.38673885886803716No Hit
TACGGC966070.3814829886118204No Hit
TCTTCA961510.37968232983132844No Hit
ATTGAA959110.37873461468370107No Hit
TCGTGG953490.37651538171300697No Hit
CTGGAG944480.3729575010962892No Hit
GCTGTC941750.3718794751158631No Hit
AACTAG936890.3699603519419177No Hit
CAGTCC934960.3691982310107007No Hit
TCCAGA928010.3664538058956964No Hit
CGTGAA911280.3598474415541107No Hit
TAGAGC898560.35482455127168566No Hit
AAGGCG893990.35301994367807854No Hit
AAGCGC892560.35245526340261724No Hit
CGAATT879570.3473257551660841No Hit
CAGCCT877280.346421476962723No Hit
ACAGCC864410.3413393544835712No Hit
GCTCAA862770.34069174913269257No Hit
CCGGAA852890.3367903217749599No Hit
GGTAAT832210.3286241762529041No Hit
CCACTT831870.32848991660699023No Hit
GCGCAG829770.3276606658528163No Hit
TTCTAC826260.32627463244941124No Hit
GAGCAC811840.32058044393741686No Hit
GGCCGT802700.3169712287502026No Hit
GACACG795690.3142031107565077No Hit
AGTTAG786020.3103846084741924No Hit
GAGTCG764840.30202102229638095No Hit
CCAGCG762930.3012667989913942No Hit
GCCATA750720.29644529817783993No Hit
ATGCCG745470.29437217129240506No Hit
TGTGAT740520.29241750880042366No Hit
GTACTT727210.28716163854420684No Hit
TTACGT726780.2869918395802569No Hit
CAACGG720660.28457516595380716No Hit
GACTTG708450.27975366514025296No Hit
GAATAC697000.2752322741234474No Hit
ACCAGG689350.2722114320903852No Hit
CAGTAG677250.2674333682210972No Hit
CGTTGC669480.2643651404306536No Hit
GGCCAG665760.26289618195183123No Hit
TCATGT659790.2605387405221081No Hit
GCTTCT650790.25698480871850554No Hit
CCGTCG639190.25240418550497323No Hit
CCTCGG628850.24832111274394533No Hit
GTGGTG627230.24768140501929686No Hit
CTGAGG605660.23916381512999593No Hit
TACAGT577220.22793339063061163No Hit
AACTCT575080.2270883446239772No Hit
ATGGCC563210.22240110345633687No Hit
AGGACT562880.22227079262353813No Hit
GAGAAG551290.21769411822312096No Hit
CAGGAC547790.21631203363283108No Hit
CAAGGA544270.2149220514163109No Hit
GAAGAT541880.21397828508179867No Hit
GTTGGC478700.18902968382050828No Hit
CGAAGC461640.18229300864612377No Hit
TCAATG456570.18029096039676096No Hit
ACACGA436150.1722274840156981No Hit
GTCGAG415700.16415216119528994No Hit
TCCACG406130.1603731470441258No Hit
CGAGCC404310.15965446305717507No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)