FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27755183
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAA3510751.264898883930976No Hit
TTAGCG3184811.1474649617694828No Hit
TCGGAC3120901.124438631876432No Hit
GATCCT3012431.0853576429310519No Hit
AATTGC3001461.0814052279893092No Hit
AGTGCA2722100.9807537568748871No Hit
AGAACG2707570.9755186986156784No Hit
CTACCG2698470.9722400317086722No Hit
TGCGAC2668340.9613844016088814No Hit
CTATTA2667250.9609916821661741No Hit
CGCTTG2642860.9522041342692642No Hit
GTACGC2593360.9343696274674176No Hit
AGTAGG2563230.9235139973676267No Hit
AATCGG2526100.9101363158009083No Hit
TATTGG2501050.9011109744799737No Hit
ATGTGA2495870.8992446563944471No Hit
GGCGTT2408680.8678307039085277No Hit
GAGCCA2389210.8608157978998012No Hit
TACGAA2364460.851898544498878No Hit
CGTCAG2346320.8453628282688678No Hit
GTTACA2309440.8320752199688253No Hit
TGTGTG2309310.8320283818701538No Hit
GAACCG2287240.8240767138879971No Hit
AGTTCC2263790.815627841473789No Hit
TTCCAT2252830.8116790294627134No Hit
TTCTGT2236410.805763017307434No Hit
TACTCG2222480.8007441348882477No Hit
TATTCT2217600.7989859047227323No Hit
TATAAG2215960.7983950240933377No Hit
TATGGT2202180.793430185634157No Hit
GCATAA2192480.7899353428871285No Hit
CTCCGC2156940.7771305272964693No Hit
GGATCG2155730.7766945726857575No Hit
CTAGTT2142090.7717801752559152No Hit
TAAGTC2125830.7659218099913087No Hit
TCCTCC2082380.7502670762430209No Hit
CGAGAG2066040.744379887533078No Hit
AAGCAT2055890.7407229129060328No Hit
AGATGC2048950.7382224790231071No Hit
CATAAC2026720.7302131641502778No Hit
AGTCAA2016840.7266534686512426No Hit
TGCGGA1998340.7199880469172191No Hit
ATTGCT1985740.7154483542767489No Hit
CCGCTC1958110.7054934568437181No Hit
ATCAGC1944610.7006295004432145No Hit
CTTCAC1938090.6982803896483046No Hit
CGACAA1924400.6933479775651272No Hit
ACCGCT1912840.6891829897140292No Hit
AGAGGT1907230.68716174560982No Hit
TCAAGC1902810.6855692502549884No Hit
AGGTCA1893460.6822005100813062No Hit
GGATTC1890920.6812853656918781No Hit
TAATGA1885690.6794010329530165No Hit
ATACCT1860940.670483779552093No Hit
GGTGGT1839730.6628419636073017No Hit
GCGTAT1839420.6627302727566236No Hit
ACGCTA1838570.6624240236499251No Hit
AGCCTA1814490.6537481666036934No Hit
AACAAT1814490.6537481666036934No Hit
TTCACA1771490.6382555647354226No Hit
GACCAT1755110.6323539643028114No Hit
AATGTA1754920.6322855086201378No Hit
GGAGGC1752750.6315036726653901No Hit
AGGTGT1741310.6273819199822966No Hit
GCACAT1738670.6264307462861982No Hit
ATCCAC1709300.6158489389171024No Hit
ACCTGC1707560.6152220289810375No Hit
CACCTA1701400.613002623690141No Hit
TGGCAT1701010.6128621093941266No Hit
CATTAA1694490.6105129985992166No Hit
AGGCTT1679210.6050077205399799No Hit
TTCGTT1678940.6049104414119698No Hit
AAGTTG1669290.6014336133182765No Hit
GGCAGG1661060.5984684013793027No Hit
ACGTAA1660270.5981837698566066No Hit
CCTGAC1654380.5960616436937202No Hit
TCTCTG1648810.5940548113121791No Hit
TGACAC1637360.5899294556984186No Hit
CTGTTC1613270.5812499957215198No Hit
AGACGG1609480.5798844849987118No Hit
CCTGCT1604790.5781947105158701No Hit
GCGGCT1573880.5670580518240503No Hit
GACCGA1562870.5630912251596396No Hit
ACTAAT1530850.5515546411637784No Hit
ATTCGA1522230.5484489149287901No Hit
TCTCGC1492580.5377662255010173No Hit
ACGAAC1477640.53238344708446No Hit
AACCTT1450820.5227203870354593No Hit
CAAGCT1444880.5205802462192377No Hit
CCGACT1421810.512268285170377No Hit
GATTCA1420210.5116918162636507No Hit
GCCTGG1415160.5098723362767956No Hit
GGTACC1412010.5087374131166781No Hit
ACTAGC1393030.5018990507106367No Hit
CTCGTC1384160.49870325120897235No Hit
CTGTAT1373180.49474723333656273No Hit
CATGAG1336650.4815857276098666No Hit
ATTCAT1333560.4804724220337513No Hit
CCATGA1317810.4747978062331637No Hit
AAGAGG1317420.4746572919371491No Hit
CTATGC1314710.47368089772638144No Hit
GTCTGC1270820.457867635028744No Hit
CATACG1258800.45353691236696225No Hit
CACATT1255490.4523443423161721No Hit
CGTCTC1231530.44371172043794493No Hit
TTGAAT1226310.4418309906297501No Hit
GGACTA1198770.43190851957272275No Hit
GAGATA1159440.41773819325925543No Hit
GAGTGA1156390.4166392994058083No Hit
CCTTAT1144310.41228695916002434No Hit
GTAAGA1141670.41133578546392574No Hit
GTCTCA1138760.4102873326398172No Hit
ATCCGG1134190.40864079332498005No Hit
CTTAGC1124010.4049730099059336No Hit
AACATA1116160.4021447093323074No Hit
TGATAA1115040.401741181097599No Hit
TGCATA1075220.38739431118144674No Hit
TTAGTA1069560.3853550524239022No Hit
TACGGC1067860.38474255421050546No Hit
CGTACT1066680.3843174083917948No Hit
TCTTCA1057720.3810891825141272No Hit
ATTGAA1048590.37779970681511993No Hit
TCGTGG1036820.37355905742001416No Hit
GCTGTC1029150.37079560959839464No Hit
CTGGAG1024470.36910943804622004No Hit
TCCAGA1024210.369015761848877No Hit
AACTAG1016150.36611179973124297No Hit
CAGTCC1006700.3627070302508904No Hit
CGTGAA987450.35577138871683894No Hit
AAGCGC985460.35505440551409806No Hit
TAGAGC985040.3549030824260824No Hit
AAGGCG978820.3526620595511836No Hit
CGAATT970190.34955273038552837No Hit
GCTCAA969030.34913479042815176No Hit
CAGCCT949550.34211628148875833No Hit
ACAGCC942810.33968790621917355No Hit
CCGGAA940290.33877996769107954No Hit
GGTAAT907350.3269119140738506No Hit
GCGCAG905030.3260760341590974No Hit
CCACTT903700.32559684438038117No Hit
TTCTAC900130.32431059813224794No Hit
GAGCAC890360.3207905348705501No Hit
GGCCGT875690.3155050355820028No Hit
GACACG872980.31452864137123504No Hit
AGTTAG843910.30405492192215056No Hit
GAGTCG833300.3002322124844214No Hit
CCAGCG832700.300016036644399No Hit
GCCATA831460.29956927324168603No Hit
NNNNNN822130.296207738929338No Hit
ATGCCG811830.2924967203422871No Hit
TGTGAT804510.28985937509401394No Hit
GTACTT801390.2887352607258976No Hit
TTACGT788210.2839865981067392No Hit
CAACGG781380.28152579646115106No Hit
GACTTG765200.27569625464188074No Hit
GAATAC761750.27445324356175205No Hit
ACCAGG759660.27370023105234076No Hit
CAGTAG735120.26485863919542524No Hit
GGCCAG728920.26262482218186056No Hit
CGTTGC726500.26175291296043696No Hit
TCATGT720990.2597676981628981No Hit
GCTTCT708230.25517035863175536No Hit
CCGTCG688040.24789604161500212No Hit
GTGGTG687990.24787802696166697No Hit
CCTCGG679860.24494884432936362No Hit
CTGAGG656090.23638467813381017No Hit
AACTCT632770.22798264381827354No Hit
TACAGT623360.22459228606058912No Hit
ATGGCC614850.2215261920629383No Hit
AGGACT608570.21926355160403732No Hit
CAAGGA602120.2169396613237967No Hit
GAGAAG600810.2164676774064145No Hit
GAAGAT593190.21372224423813024No Hit
CAGGAC589550.21241077747532777No Hit
GTTGGC538070.1938628904014072No Hit
CGAAGC500850.1804527824586853No Hit
TCAATG493150.1776785258450647No Hit
ACACGA477160.17191743970846815No Hit
GTCGAG449920.16210305657145188No Hit
TCCACG442210.15932519702716424No Hit
CGAGCC439100.1582046855897149No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)