FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002208472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21710605
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA6407892.9515022727372173No Hit
AATCGG5786952.665494582025697No Hit
TATTCT4449472.049445420797808No Hit
AGTCAA3738501.7219695167407818No Hit
TTCGTT3697891.7032643724115473No Hit
ACCGCT3656261.6840894116032235No Hit
ATCCAC3454631.591217748192646No Hit
GGATTC3419111.5748570802149457No Hit
TGTGTG3310211.5246972620062869No Hit
AAGTTG3297991.5190686763450396No Hit
GACCGA3231731.4885490293798815No Hit
AAGCAT3112751.4337463189072805No Hit
GTAAGA3008761.3858480682597283No Hit
AGACGG2877801.325527317179784No Hit
ACTAGC2761341.2718853297731685No Hit
GGACTA2712831.2495414107529477No Hit
GATCCT2681281.2350093422085657No Hit
GGATCG2678951.2339361339769204No Hit
CATAAC2678441.2337012257373758No Hit
AACAAT2660831.2255899824072154No Hit
CCTTAT2585381.19083738108634No Hit
AATGTA2543721.1716486021462782No Hit
TTCTGT2543391.1714966026971612No Hit
ATGGCC2402431.1065698077045758No Hit
CACATT2390241.1009550401750665No Hit
GGTACC2373121.0930694929966254No Hit
TCAATG2363041.0884266007326833No Hit
AGGACT2242911.033094195210129No Hit
GAGCCA2240181.0318367452219779No Hit
CGAGAG2208511.0172494041506444No Hit
AGATGC2202831.0146331712082644No Hit
TATTGG2156450.9932703395414361No Hit
TCGTGG2139150.9853018835725674No Hit
GAGCAC2121910.9773610638671746No Hit
GCTTCT2097010.965892014524699No Hit
TAAGTC2070940.9538840580444442No Hit
GAGATA2056320.9471500218441632No Hit
GGCGTT2029740.9349071571243638No Hit
CATTAA2019920.9303840220021505No Hit
AGCCTA2009490.9255799182012662No Hit
TATAAG2008090.9249350720534965No Hit
CGTTGC2004780.9234104715184124No Hit
ACCTGC2001240.9217799319733374No Hit
GAATAC1976930.9105826392217076No Hit
TATGGT1954110.9000716470130612No Hit
CGAATT1953800.8999288596517693No Hit
CAAGCT1930980.8894178674431229No Hit
GGTGGT1916690.882835830691959No Hit
GTTACA1902470.8762860362481837No Hit
CCGACT1863700.8584284039988751No Hit
ATGTGA1803870.8308704432695451No Hit
CAGTAG1799080.82866414823539No Hit
ATACCT1786700.8229618658715406No Hit
GAAGAT1767440.8140906252957945No Hit
TGGCAT1757510.809516823690542No Hit
TCAAGC1748820.8055141715304571No Hit
TAATGA1701430.7836861294284521No Hit
AGTAGG1686470.7767954877351414No Hit
ACAGCC1669260.7688684861614865No Hit
CTAGTT1661160.7651375905922474No Hit
CACCTA1655810.7626733570989845No Hit
CCTGCT1643970.7572198011064177No Hit
TACGGC1609580.7413796160908459No Hit
GCATAA1590240.7324715271637985No Hit
CTGGAG1583970.7295835376305727No Hit
GGCCAG1562570.7197266036575213No Hit
ATCAGC1551940.7148303789783841No Hit
AACTAG1525210.702518423599895No Hit
CTATGC1520660.7004226736196435No Hit
GACCAT1505330.6933616083015651No Hit
AGAACG1495240.6887141099937105No Hit
TGCGAC1478010.6807778963322303No Hit
ATGCCG1409270.6491159504767371No Hit
ACACGA1396620.6432893049272465No Hit
CAGGAC1396130.643063608775527No Hit
GCACAT1348720.6212263545856967No Hit
CGAGCC1342600.6184074557111605No Hit
ACGTAA1318570.6073391321890845No Hit
TTAGTA1305850.6014802443322054No Hit
CTGTTC1280470.5897901048819228No Hit
AAGAGG1220940.5623703254699719No Hit
CCATGA1144630.5272216043726097No Hit
ATCCGG1123220.5173600643556455No Hit
TCCACG1121380.5165125522757197No Hit
CCTCGG1116550.5142878330659141No Hit
CGTCAG1092150.5030490859190704No Hit
GTACGC1083870.4992352815594038No Hit
TCTCTG1065430.4907417365844941No Hit
ACGAAC1061140.4887657437459712No Hit
GAGTGA1051360.4842610327994084No Hit
CAACGG1011940.46610400769577814No Hit
TTCACA993510.45761506876478103No Hit
TGCATA973900.44858261665209237No Hit
CATACG949670.4374221722517636No Hit
AACCTT930700.4286845069494839No Hit
GAGAAG898310.4137655307164402No Hit
GAGTCG872750.40199248247573016No Hit
AGGTCA871720.4015180599527282No Hit
CAGCCT844000.3887501062268877No Hit
GGAGGC837240.3856364205419425No Hit
ACGCTA805470.37100301903148253No Hit
CTACCG773880.3564525263114501No Hit
NNNNNN768840.3541310801794791No Hit
CTCGTC664830.3062236174441016No Hit
CGTGAA639090.2943676604129641No Hit
TTGAAT572630.26375589256955295No Hit
TTACGT563470.259536756345574No Hit
AAGGCG533850.24589365427633178No Hit
TTCCAT520460.23972616147730566No Hit
AGTGCA483020.22248113306837836No Hit
TACGAA408640.1882213784461557No Hit
TCGGAC362250.1668539407354148No Hit
GAACCG325820.15007412276166418No Hit
CGACAA290590.13384703005743046No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)