FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27311882
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGT6437162.3569082496768257No Hit
CTATTA6345872.3234832370760827No Hit
ATGTGA5342801.9562181763966322No Hit
TATAAG5052501.849927441836487No Hit
AAGCAT4712761.7255346958514246No Hit
AGAACG4472131.637430185147988No Hit
ATACCT4447461.6283974864859183No Hit
AATCGG4317081.5806600218908384No Hit
TGCGAC4307611.5771926665471094No Hit
CTAGTT4116391.5071791830383567No Hit
CATAAC3846491.408357725037037No Hit
ATCAGC3833331.4035393093745792No Hit
GACCAT3745161.3712566567181272No Hit
TATTCT3274591.1989616826844816No Hit
AGATGC3119931.1423343144203684No Hit
TCGTGG3065451.1223869523162118No Hit
TAAGTC3063171.1215521508184607No Hit
TATGGT3058041.1196738474485208No Hit
AGACGG3050291.1168362546381827No Hit
TTCGTT2972931.0885115862758925No Hit
GTTACA2954191.0816501037899915No Hit
CGTCAG2927011.0716983911983802No Hit
TTCACA2913781.0668543456653774No Hit
GTACGC2877441.0535487814424507No Hit
TCTCTG2868731.0503596932646384No Hit
ACCTGC2832231.0369955464804659No Hit
CCTTAT2783601.0191901092718547No Hit
AACAAT2762771.0115633920796816No Hit
AGTCAA2762661.0115231165688252No Hit
CAAGCT2726580.9983127490079227No Hit
ACCGCT2701360.989078672791571No Hit
ACAGCC2686410.9836048647251772No Hit
AGTAGG2645290.9685491464850353No Hit
TGTGTG2634530.9646094692412628No Hit
TCAAGC2632870.9640016751683389No Hit
AAGTTG2622380.9601608559966683No Hit
TGGCAT2603700.9533213419712343No Hit
GATCCT2592240.949125366022012No Hit
GGATCG2583950.9460900570674698No Hit
TACGGC2581240.9450978149363709No Hit
GACCGA2572210.9417915616360674No Hit
ATGGCC2568990.940612587590998No Hit
ATCCAC2553640.9349923231214897No Hit
TAATGA2546170.9322572497933317No Hit
GGATTC2521270.9231403386994715No Hit
AGGTCA2493640.9130238626543568No Hit
GCATAA2478570.9075061176670287No Hit
AACTAG2441020.8937575228246811No Hit
GAGAAG2438740.8929227213269301No Hit
CCTGCT2357270.8630932134226413No Hit
CACCTA2342920.857839089960919No Hit
GGTACC2309180.8454854923582343No Hit
CAGCCT2295730.8405608958035188No Hit
CTATGC2255140.8256992322975033No Hit
GTAAGA2237210.8191343240279085No Hit
ACTAGC2228010.8157658267562814No Hit
CTACCG2215190.8110718990364706No Hit
GGAGGC2206850.8080182830315391No Hit
GAGCCA2160360.790996387579589No Hit
CGAGAG2143590.7848562028790254No Hit
ACGCTA2130700.7801366452886696No Hit
GAATAC2109720.7724550069453288No Hit
GGCGTT2087100.7641728973492196No Hit
TATTGG2069660.7577873981734397No Hit
ACGTAA2057020.7531593758350303No Hit
AGCCTA2056240.7528737858489576No Hit
GGACTA2035210.7451738404552275No Hit
AATGTA2024920.7414062494851141No Hit
GGTGGT1943810.7117085523436284No Hit
CTGTTC1932780.7076700170277537No Hit
CAGTAG1926400.705334037398082No Hit
GCACAT1912540.7002593230301741No Hit
CCGACT1903890.6970922033128292No Hit
TCAATG1900940.6960120873398619No Hit
TTAGTA1850660.6776025174684044No Hit
AGGACT1807670.6618621155437037No Hit
CACATT1797630.6581860598255367No Hit
CCTCGG1786250.6540193751569372No Hit
CTCGTC1781870.6524156775428365No Hit
AAGAGG1758930.644016402824236No Hit
CGTGAA1729010.6330614638712924No Hit
GAGCAC1710290.6262073042055468No Hit
GAAGAT1709300.6258448246078392No Hit
ATCCGG1696740.6212460935500528No Hit
GCTTCT1687880.6180020842210727No Hit
GGCCAG1671040.6118362696499641No Hit
CCATGA1626540.5955429948035071No Hit
CAACGG1624410.594763114456924No Hit
CGTTGC1623240.5943347294778147No Hit
ACGAAC1609900.5894504084339556No Hit
GAGTGA1609740.5893918258727099No Hit
CATTAA1591390.582673138379845No Hit
TTGAAT1546590.5662700212310525No Hit
GAGATA1518880.556124253905315No Hit
TTACGT1510440.5530340237996049No Hit
ATGCCG1494120.5470586025525447No Hit
TGCATA1464190.5361000021895233No Hit
AAGGCG1458580.5340459511358463No Hit
CGAATT1455900.533064693234981No Hit
CATACG1427230.5225674305417694No Hit
AACCTT1409010.5158963413799166No Hit
GAGTCG1393210.510111313456905No Hit
CAGGAC1370680.5018621565514966No Hit
ACACGA1344830.4923974115002401No Hit
CGAGCC1299350.47574531846615326No Hit
CTGGAG1196380.4380437788944753No Hit
TCCACG1086340.39775362239775347No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)