FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23062230
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA3234211.402383897827747No Hit
CTTAGC2343181.0160249030557755No Hit
TATTCT2285070.9908278600985247No Hit
AATCGG2255680.9780840794667298No Hit
TGATAA2246110.9739344373896193No Hit
CACCTA2170750.9412576320676708No Hit
TAGAGC2143480.929433103390262No Hit
CGAGAG2112970.9162036802165272No Hit
GCATAA2059060.8928277967915504No Hit
TAAGTC2045970.8871518495826294No Hit
TGTGTG2024830.8779853466035157No Hit
TTCTAC1995180.8651288275244848No Hit
ACCGCT1942440.8422602671120702No Hit
CAAGCT1915890.8307479372116227No Hit
TATAAG1893230.8209223479255909No Hit
GGCCGT1874710.8128919016070866No Hit
CAGTCC1812910.7860948399179091No Hit
AGTGCA1789620.7759960767020362No Hit
GACCGA1741570.7551611444339945No Hit
TACAGT1717390.7446764688410444No Hit
ACGCTA1706220.7398330517040199No Hit
TATGGT1690530.7330297200227385No Hit
GAGTGA1665250.7220680740760976No Hit
TCGGAC1644480.7130620065795892No Hit
ATCCAC1640260.7112321748590661No Hit
GACTAA1625060.7046413117898833No Hit
GTAAGA1614770.7001794709358116No Hit
TCTCTG1596440.6922314104056719No Hit
GTTACA1585280.687392329362772No Hit
AAGTTG1584680.6871321637153042No Hit
TTACGT1579620.6849381000883262No Hit
AAGCAT1577450.683997167663318No Hit
ACCTGC1573430.682254057825284No Hit
CAGGAC1565570.6788458878434567No Hit
TAATGA1554520.6740545038359257No Hit
ATTGCT1544460.6696923931467165No Hit
AGCCTA1542440.6688165021335751No Hit
CCGCTC1521920.6599188369901784No Hit
TGCGGA1521700.6598234429194401No Hit
ATACCT1499030.6499935175392839No Hit
TTCACA1492370.647105678852392No Hit
TTCGTT1491530.6467414469459372No Hit
AAGCGC1491390.6466807416281947No Hit
AGTAGG1483680.6433376130582342No Hit
TTCCAT1455680.6311965495097396No Hit
ACCAGG1447820.6277883795279121No Hit
GGAGGC1441070.6248615159938999No Hit
GGCAGG1423410.6172039737700994No Hit
AGATGC1419820.6156473159794175No Hit
TGTGAT1419180.6153698059554519No Hit
CGTACT1400710.6073610401075697No Hit
CATGAG1396760.6056482829284072No Hit
GGATTC1392020.603592974313412No Hit
ATCCGG1383790.6000243688489795No Hit
TCCAGA1366630.592583631331402No Hit
AACTAG1366020.5923191295898098No Hit
CAACGG1362760.5909055629052351No Hit
TCTTCA1360440.5898995890683598No Hit
TCCTCC1357670.5886984909958837No Hit
CGTCTC1355840.5879049857711072No Hit
TACTCG1349980.5853640346141722No Hit
GATCCT1342300.582033914326585No Hit
CCGTCG1328570.5760804570936983No Hit
CTCCGC1325540.5747666205739861No Hit
GTACGC1322500.5734484479601496No Hit
CATAAC1317410.5712413760507983No Hit
TATTGG1317090.5711026210388154No Hit
GGATCG1312310.5690299680473224No Hit
TGCGAC1307910.5671220866325589No Hit
AATTGC1301290.5642515923221648No Hit
TTAGTA1298390.5629941250260707No Hit
GCACAT1291240.5598938177270801No Hit
TTGAAT1285670.5574786132997546No Hit
AGGCTT1270250.5507923561598336No Hit
CGAATT1266310.5490839350747955No Hit
TGACAC1265870.5488931469333191No Hit
CCTTAT1255500.5443966173262516No Hit
GGTGGT1251120.5424974080997371No Hit
AGACGG1246990.5407066012263342No Hit
GCTGTC1243960.5393927647066221No Hit
AATGTA1237520.5366003200904683No Hit
TACGAA1237070.5364051958548675No Hit
GTCTGC1236910.5363358183488761No Hit
CCATGA1231190.5338555725096836No Hit
GGTACC1228590.5327281880373234No Hit
CATTAA1222390.5300398096801567No Hit
ACACGA1221920.5298360132563069No Hit
TGGCAT1215720.5271476348991403No Hit
CGTTGC1206690.5232321419047508No Hit
ACAGCC1200830.5206911907478159No Hit
GCGCAG1196040.5186142016621983No Hit
ACTAGC1193100.5173393899896064No Hit
AACATA1191360.5165849096119499No Hit
AGTTCC1187730.5150109074447701No Hit
TCAAGC1186220.5143561572319763No Hit
AGAACG1179400.5113989410390929No Hit
GTACTT1173900.5090140892706386No Hit
CGAAGC1167170.5060958979248754No Hit
ACGTAA1166980.5060135121365107No Hit
AGGTCA1159800.5029001965551467No Hit
GAGCCA1159200.5026400309076788No Hit
CCAGCG1158810.502470923236825No Hit
ATCAGC1150500.49886762901939663No Hit
CCTGCT1146360.4970724860518692No Hit
TCGTGG1133080.49131415305458315No Hit
CGCTTG1129780.48988324199351063No Hit
TCAATG1122880.48689133704763154No Hit
AGTCAA1119390.4853780401981942No Hit
GGTAAT1113580.48285876951188156No Hit
GTGGTG1103840.4786354138346552No Hit
GCTCAA1098790.4764456863018017No Hit
TTCTGT1086640.4711773319405799No Hit
GGACTA1082780.4695035996085374No Hit
GTCTCA1079360.4680206554179713No Hit
GAGAAG1075370.46629055386231083No Hit
ATTCGA1070130.4640184405410925No Hit
ACTAAT1068380.4632596240693116No Hit
GAACCG1063850.46129537343093013No Hit
CTAGTT1059050.45921404825118817No Hit
CCACTT1056190.457973925331592No Hit
CTATGC1051530.4559533054695925No Hit
CGTCAG1050980.455714820292747No Hit
AACCTT1043500.45247142188764916No Hit
AGGACT1032480.44769304616249167No Hit
GAATAC1022880.4435303958030078No Hit
CTGTAT1016950.44095909198720157No Hit
ATTCAT1016150.4406122044572446No Hit
TCTCGC1002560.4347194525421002No Hit
AAGAGG1000300.43373949526997174No Hit
CTGTTC997620.4325774220446158No Hit
GACACG986330.4276819717780978No Hit
CGAGCC985900.4274955197307459No Hit
CCGACT981730.4256873684808451No Hit
GAGATA965730.4187496178817053No Hit
ATGTGA964890.4183853859752505No Hit
AACTCT946150.41025954558600797No Hit
AGTTAG941770.4083603363594934No Hit
GCTTCT930550.4034952387518467No Hit
GACCAT927060.4019819419024092No Hit
GCGTAT923260.4003342261351135No Hit
CGACAA919230.3985867802029552No Hit
TACGGC914350.3964707662702176No Hit
GATTCA910520.39481004222054844No Hit
GAGCAC905650.3926983643819353No Hit
CTTCAC894470.38785061115078634No Hit
CTGGAG888170.3851188718523751No Hit
GAGTCG874950.37938655541983585No Hit
CTGAGG865900.37546239023719735No Hit
CTACCG864650.37492037847163956No Hit
CCGGAA859690.37276967578590625No Hit
TTAGCG842370.3652595607623374No Hit
AACAAT842060.36512514184447903No Hit
GACTTG837570.36317823558259543No Hit
CCTCGG832500.360979835861493No Hit
AGGTGT832340.3609104583555016No Hit
CACATT831870.3607066619316519No Hit
CTCGTC831640.36060693176678926No Hit
ATGCCG820310.3556941371237734No Hit
AGAGGT813120.3525764854482849No Hit
TCATGT803720.3485005569712903No Hit
CAGCCT803150.34825339960619595No Hit
GCCTGG798560.3462631324030677No Hit
GGCGTT792730.3437351895285061No Hit
GTCGAG789270.34223490096144216No Hit
CCTGAC768660.3332982109709252No Hit
GCGGCT735000.3187029181479848No Hit
GAAGAT730090.3165738959328738No Hit
TGCATA723260.313612343645866No Hit
ACGAAC716880.31084591559445895No Hit
ATGGCC677630.2938267461559442No Hit
CATACG673130.2918755037999361No Hit
CAGTAG662530.287279244028006No Hit
CAAGGA654440.2837713438813159No Hit
AAGGCG646530.28034149342886616No Hit
TCCACG608870.26401176295614087No Hit
GTTGGC592930.2571000289217478No Hit
ATTGAA584740.25354876783381314No Hit
CGTGAA580910.25188804378414403No Hit
GCCATA513400.22261507234989852No Hit
GGCCAG491400.2130756652760813No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)