FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26821599
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA3804951.4186141549577265No Hit
CTTAGC2768081.0320339216166792No Hit
TATTCT2687951.0021587452709289No Hit
AATCGG2637940.9835133244665987No Hit
TGATAA2572950.9592828525995039No Hit
CACCTA2538330.9463753447361584No Hit
TAGAGC2534950.9451151663254678No Hit
CGAGAG2487250.9273309917130592No Hit
GCATAA2417150.9011953388759559No Hit
TGTGTG2385790.889503269361383No Hit
TAAGTC2343810.8738517043670662No Hit
TTCTAC2288820.8533495709931388No Hit
ACCGCT2264740.8443717319015918No Hit
CAAGCT2254150.8404234214373274No Hit
GGCCGT2211740.8246115378878045No Hit
TATAAG2162520.8062606558244346No Hit
CAGTCC2053680.7656814196648007No Hit
AGTGCA2041530.7611514883956023No Hit
GACCGA2040160.7606407060220384No Hit
TACAGT2021980.7538625866414601No Hit
TATGGT1999630.745529750109231No Hit
GAGTGA1949230.7267389241036674No Hit
ACGCTA1945950.7255160290779084No Hit
ATCCAC1926250.7181712022463687No Hit
GTAAGA1897820.7075715359102939No Hit
TCGGAC1879870.7008791683150584No Hit
AAGTTG1858250.6928185004928304No Hit
GACTAA1858090.6927588470769398No Hit
ACCTGC1855170.6916701722369348No Hit
GTTACA1851210.6901937501936406No Hit
CAGGAC1850080.6897724479439127No Hit
TCTCTG1825820.6807274987594886No Hit
TTACGT1818190.6778827764892019No Hit
AAGCAT1808700.674344583259186No Hit
TAATGA1782360.6645241396681831No Hit
TTCGTT1759650.6560570829501999No Hit
ATTGCT1756890.6550280615260857No Hit
AAGCGC1756100.6547335227851255No Hit
AGCCTA1750560.6526680232599108No Hit
AGTAGG1748180.651780678698537No Hit
TGCGGA1743790.6501439381000365No Hit
CCGCTC1734240.6465833748390616No Hit
ATACCT1710520.6377397559332686No Hit
TTCACA1700960.6341754643338005No Hit
AGATGC1680120.6264056069140397No Hit
TTCCAT1664650.6206378672651097No Hit
ACCAGG1661560.6194858106707211No Hit
GGCAGG1651770.6158357672859102No Hit
GGAGGC1646710.6139492280083674No Hit
GGATTC1639950.6114288711869862No Hit
TGTGAT1622290.6048446254080526No Hit
AACTAG1611920.6009783383906381No Hit
CGTACT1603700.5979136441492545No Hit
TCCAGA1597720.5956840977303404No Hit
CAACGG1594310.5944127343041703No Hit
CGTCTC1592390.5936968933134822No Hit
CATGAG1591510.5933687995260835No Hit
TACTCG1583790.5904905222093583No Hit
GATCCT1576730.587858315233182No Hit
ATCCGG1575270.5873139778131796No Hit
TATTGG1554240.5794732819620485No Hit
CATAAC1551620.5784964572768387No Hit
TCCTCC1550240.5779819465647815No Hit
CTCCGC1546420.5765577212603917No Hit
TCTTCA1545470.5762035291035408No Hit
GGATCG1542780.5752006060488787No Hit
TTAGTA1535450.572467733933387No Hit
CCGTCG1522930.5677998541399415No Hit
GCACAT1512590.5639447521380064No Hit
GTACGC1510200.5630536792381394No Hit
TGCGAC1499110.5589189518492167No Hit
TGACAC1493450.556808712262084No Hit
CGAATT1490860.5558430725923537No Hit
AGGCTT1484790.553579971127001No Hit
AATTGC1484390.5534308375872743No Hit
TTGAAT1480440.5519581438824732No Hit
AATGTA1453480.541906543304894No Hit
CCATGA1449480.540415207907627No Hit
GGTACC1447840.5398037603947475No Hit
ACACGA1442080.5376562374226832No Hit
CCTTAT1437410.5359151033463739No Hit
AGACGG1431140.533577435111158No Hit
CATTAA1427050.5320525446674526No Hit
CGTTGC1423630.5307774529027893No Hit
GGTGGT1422260.5302666705292254No Hit
GCTGTC1417880.528633658269218No Hit
GTCTGC1416290.5280408524488045No Hit
ACAGCC1413390.5269596342857858No Hit
TACGAA1410830.526005179631535No Hit
TGGCAT1410050.5257143692290679No Hit
AGTTCC1403460.5232573941620706No Hit
ACTAGC1399480.5217735154417901No Hit
GTACTT1385560.516583668259301No Hit
ACGTAA1371410.5113080692914692No Hit
TCAAGC1367060.5096862420469414No Hit
AACATA1362300.5079115529241938No Hit
GCGCAG1358940.5066588311904895No Hit
GAGCCA1355560.5053986527797988No Hit
CCTGCT1349350.503083354575542No Hit
AGAACG1336700.4983670063816852No Hit
TCGTGG1334230.4974461067738728No Hit
CGAAGC1333670.4972373198182554No Hit
CCAGCG1322250.49297955725905823No Hit
AGGTCA1316480.4908283059485007No Hit
TCAATG1314950.49025787015904604No Hit
ATCAGC1313870.48985520960178397No Hit
GGTAAT1312810.4894600057215082No Hit
AGTCAA1308420.4878232651230077No Hit
CGCTTG1278580.47669790305939624No Hit
GGACTA1270640.4737376022958214No Hit
GTCTCA1267800.4726787541637618No Hit
GTGGTG1262170.4705796995921086No Hit
GCTCAA1248010.4653003722857835No Hit
TTCTGT1242490.46324232943755517No Hit
ATTCGA1224750.45662825695067616No Hit
GAGAAG1223630.45621068303944146No Hit
ACTAAT1222090.4556365189114937No Hit
CCACTT1212640.4521132390354505No Hit
AGGACT1209530.45095372576407544No Hit
CTATGC1209500.4509425407485959No Hit
GAACCG1207110.45005146784872896No Hit
CTAGTT1204410.4490448164555737No Hit
CGTCAG1199370.44716573385501734No Hit
AACCTT1193690.4450480375908982No Hit
TCTCGC1185210.4418864065486924No Hit
GAATAC1175540.43828110322579944No Hit
AAGAGG1173390.43747951044976846No Hit
ATTCAT1167690.4353543575086631No Hit
CGAGCC1166220.4348062917501675No Hit
CTGTAT1163110.4336467784787924No Hit
CTGTTC1146660.4275136616575321No Hit
GAGATA1136940.42388971664217334No Hit
GACACG1123480.41887137303037003No Hit
CCGACT1118710.4170929555691292No Hit
AACTCT1118380.4169699203988546No Hit
ATGTGA1100510.4103073795115646No Hit
GCTTCT1095990.40862217051265287No Hit
TACGGC1083920.4041220659513998No Hit
GAGCAC1064450.39686299090520294No Hit
AGTTAG1061870.3959010795739657No Hit
CTTCAC1052630.39245609480627913No Hit
GACCAT1052360.3923554296669635No Hit
CGACAA1052190.3922920479125797No Hit
GCGTAT1049850.39141961670517855No Hit
CTGGAG1047570.39056955552873635No Hit
GATTCA1032760.38504788622035546No Hit
GAGTCG1024920.38212486884171226No Hit
TTAGCG991960.3698362651682325No Hit
CTGAGG991190.3695491831042586No Hit
CTACCG989910.3690719557771332No Hit
CCTCGG983320.3666149807101359No Hit
AGGTGT980270.36547783746971985No Hit
CCGGAA976880.3642139307205361No Hit
CACATT974200.36321473600436727No Hit
AACAAT960830.35822994743900244No Hit
AGAGGT957780.35709280419858636No Hit
GACTTG952010.3549415528880288No Hit
CTCGTC949340.3539460865103531No Hit
ATGCCG932210.34755944267155736No Hit
GTCGAG927870.3459413437655227No Hit
CAGCCT920600.34323084168099No Hit
TCATGT914500.3409565552001579No Hit
GGCGTT902360.33643035226945267No Hit
CCTGAC901780.336214108636849No Hit
GCCTGG901260.3360202350352043No Hit
GAAGAT858540.3200927729923932No Hit
GCGGCT838110.3124757774508522No Hit
TGCATA835930.31166299965934174No Hit
ACGAAC817610.30483268353985904No Hit
CATACG777550.2898969595362305No Hit
ATGGCC776510.2895092123329411No Hit
CAAGGA764240.2849345410018247No Hit
CAGTAG753910.2810831673383828No Hit
AAGGCG738680.27540490781328886No Hit
TCCACG710440.26487607990858414No Hit
GTTGGC679480.25333314393373785No Hit
CGTGAA662590.24703598021877815No Hit
ATTGAA661420.24659976461507757No Hit
GCCATA587330.21897650471920035No Hit
GGCCAG562290.20964074513230924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)