FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22376520
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTA3808291.7019134342605553No Hit
TGATAA3353861.4988300236140384No Hit
TATAAG2793331.2483308396479882No Hit
TAAGTC2627681.174302349069471No Hit
CAGTCC2343021.0470886447043597No Hit
GACTAA2296201.0261649264496893No Hit
AGTGCA2277441.0177811384433326No Hit
CTATTA2259611.0098129646611715No Hit
ATACCT2218580.9914767801248808No Hit
TTCTAC2195840.9813143419977726No Hit
TGCGGA2163990.9670806720616074No Hit
CCGCTC2122190.9484003768235633No Hit
TCGGAC2080640.9298318058393351No Hit
ACCAGG2040470.9118799527361717No Hit
AAGCAT2024520.9047519453427074No Hit
TAATGA1992550.8904646477647105No Hit
CATGAG1973170.88180378360889No Hit
AGCCTA1960880.8763114192912927No Hit
TCCTCC1892640.8458151669696629No Hit
TATTCT1872090.8366314333059833No Hit
GTCTGC1872040.83660908845522No Hit
AATCGG1860270.8313491105855603No Hit
TTCCAT1811790.809683543285551No Hit
ATCCGG1796960.8030560605491828No Hit
ACGCTA1795780.802528722071171No Hit
GCTGTC1750460.7822753493393968No Hit
CCGTCG1734200.7750088038712007No Hit
TAGAGC1717570.7675769065073568No Hit
ATCAGC1708670.7635995230715054No Hit
ATTGCT1682430.7518729453909723No Hit
TCTCTG1680460.7509925582709018No Hit
TTACGT1660970.7422825354434023No Hit
AACATA1657950.7409329064573044No Hit
CTTAGC1629230.7280980241789161No Hit
TGGCAT1618050.7231017155482622No Hit
GGAGGC1611690.7202594505311818No Hit
TTCTGT1608810.7189723871272208No Hit
GGTGGT1600000.7150352244227431No Hit
CCTTAT1598850.7145212928551893No Hit
ACCGCT1597350.7138509473322929No Hit
AGACGG1596940.7136677195560347No Hit
ACTAAT1585720.7086535350447701No Hit
TGTGAT1570820.7019947695173333No Hit
CTAGTT1554540.6947192861088319No Hit
TACGAA1550200.6927797530625852No Hit
ATTCAT1529100.6833502260405103No Hit
TCAAGC1525590.681781617516933No Hit
CGAAGC1524340.6812229962478527No Hit
TTCACA1523090.6806643749787724No Hit
CTGTAT1511660.6755563420943025No Hit
CGAGAG1479710.6612779824566107No Hit
TGTGTG1474430.6589183662160156No Hit
CGTACT1444920.6457304352955687No Hit
AATTGC1424150.636448384288531No Hit
TCTTCA1398570.6250167586380725No Hit
GTACGC1396350.6240246472641858No Hit
GACACG1392530.6223175006658765No Hit
GACCAT1373660.6138845539878408No Hit
TACAGT1363410.6093038595813827No Hit
TGCGAC1356070.6060236354893433No Hit
TTGAAT1353480.604866172219809No Hit
ATCCAC1349630.6031456187110418No Hit
AACCTT1343760.6005223332314408No Hit
CTATGC1342650.6000262775444976No Hit
GTAAGA1337610.5977739165875658No Hit
GAATAC1310570.5856898212948216No Hit
GGCCGT1307460.5842999715773498No Hit
GAACCG1300620.5812431959929426No Hit
CTGTTC1298750.5804074985743984No Hit
GCGTAT1285390.5744369544504686No Hit
GACCGA1251680.559372056065912No Hit
GTGGTG1236760.5527043525981699No Hit
CCGACT1236020.5523736488068743No Hit
GCGCAG1228470.5489995763416295No Hit
AGTTAG1227150.5484096722814807No Hit
CGCTTG1219780.5451160412789835No Hit
AGAACG1204660.5383589584081886No Hit
CCAGCG1200980.5367143773920163No Hit
AGGTCA1194690.5339033951660044No Hit
ATTCGA1172190.5238482123225595No Hit
CCACTT1163290.519870828886708No Hit
GGATTC1162230.5193971180505279No Hit
CGACAA1155590.5164297218691736No Hit
GGCAGG1153430.5154644243162029No Hit
GAGAAG1148380.5132075943891186No Hit
GCTCAA1136190.5077599197730478No Hit
AAGTTG1131910.5058472005477169No Hit
CGTCAG1103400.49310616664253426No Hit
GCATAA1083580.4842486677999975No Hit
GACTTG1082890.48394030885946515No Hit
CAGGAC1080090.48268899721672537No Hit
TTCGTT1066570.4766469495703532No Hit
AACAAT1051030.46970216995314734No Hit
CACCTA1049040.46881284489277153No Hit
AAGCGC1043330.4662610629356129No Hit
ATGCCG1041260.46533598611401594No Hit
GCGGCT1039900.4647282061732566No Hit
CGAATT1036480.463199818381053No Hit
TGACAC1010960.4517950065515103No Hit
GGCGTT1006290.44970799749022633No Hit
CGTCTC994970.4446491232774355No Hit
GATTCA988030.44154765799150175No Hit
ATGTGA980540.4382003993471728No Hit
CTGAGG959560.4288244999669296No Hit
AGTTCC955910.4271933258612152No Hit
GTACTT949100.42414995718726595No Hit
TCCAGA946860.42314890787307413No Hit
TACTCG941220.4206284087069839No Hit
TGCATA937950.41916705546707No Hit
GATCCT930850.41599408665869403No Hit
CTCCGC929810.41552931376281926No Hit
GGATCG920890.4115429923866624No Hit
ACGAAC920760.4114848957746781No Hit
CATACG920730.4114714888642202No Hit
AGTCAA917740.41013526678858014No Hit
CTACCG915350.40906718292209865No Hit
TATTGG913750.40835214769767597No Hit
CAAGCT898450.40151462336413346No Hit
GGACTA897010.400871091662153No Hit
CCGGAA885930.3959194727330255No Hit
AATGTA885150.3955708930611195No Hit
TATGGT881430.3939084361643365No Hit
TCATGT872920.3901053425644381No Hit
GCCTGG871040.38926517617574136No Hit
CATTAA868620.388183685398802No Hit
GTCTCA867850.38783957469704855No Hit
ATTGAA865060.38659273202446137No Hit
GAGTGA863440.3858687588597333No Hit
CTCGTC862530.3854620825758429No Hit
ATGGCC861750.3851135029039368No Hit
CGTTGC861720.3851000959934789No Hit
GGTACC860970.38476492323203076No Hit
GTTGGC851120.3803629876316782No Hit
ACACGA849880.3798088353327506No Hit
ACTAGC842640.3765733009422377No Hit
CAGCCT840940.3758135760162885No Hit
CAGTAG839300.3750806649112552No Hit
GTTACA821420.36709014627833103No Hit
GAGCCA811890.3628312177228631No Hit
GAGATA799600.3573388534052659No Hit
TCAATG791710.3538128359548312No Hit
GGTAAT782490.34969244547409517No Hit
AGTAGG770950.34453525391794615No Hit
AACTCT754950.3373849016737187No Hit
ACCTGC746920.33379631864114706No Hit
AGGACT738250.32992172151880633No Hit
CTGGAG737520.3295954866976634No Hit
CTTCAC712100.31823536456964713No Hit
CAACGG709190.31693489425522825No Hit
AACTAG709170.316925956314923No Hit
TCTCGC699020.31238995160999117No Hit
CGAGCC690190.3084438509652082No Hit
AAGGCG685970.3065579455607932No Hit
CACATT681550.30458266075332535No Hit
AGATGC677170.3026252518264681No Hit
GCTTCT665810.29754850173306663No Hit
GCCATA656920.29357558726736777No Hit
GAGCAC648460.2897948385182325No Hit
TTAGCG643330.2875022568299271No Hit
GGCCAG629320.2812412296460754No Hit
ACAGCC619690.276937611389081No Hit
TTAGTA616620.2755656375522199No Hit
CATAAC616570.2755432927014567No Hit
CGTGAA614390.2745690572081807No Hit
GCACAT612270.2736216355358206No Hit
CCTGAC605430.27056485995141333No Hit
AGGCTT603020.2694878381446266No Hit
ACGTAA602430.26922416890562073No Hit
CCATGA579630.2590349169575966No Hit
AGGTGT576910.25781935707607795No Hit
AGAGGT573200.2561613691494477No Hit
GTCGAG558470.24957857611460585No Hit
CCTGCT535100.23913459286788114No Hit
TCGTGG532410.23793243989682045No Hit
CAAGGA518480.23170716447418993No Hit
GAAGAT503910.2251958749617903No Hit
TACGGC495990.22165645060089773No Hit
AAGAGG488240.21819299873260004No Hit
GAGTCG458860.20506316442413744No Hit
CCTCGG428430.1914640882496474No Hit
TCCACG419910.1876565256795963No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)