FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28525837
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTA4057471.422384205588779No Hit
CTTAGC2954561.0357487494582545No Hit
TATTCT2856431.0013483565793355No Hit
AATCGG2804400.9831087515503927No Hit
TGATAA2752370.96486914652145No Hit
CACCTA2728250.9564136540498356No Hit
TAGAGC2686830.9418934841421129No Hit
CGAGAG2661640.9330628931238723No Hit
GCATAA2573250.9020769486974213No Hit
TGTGTG2570260.9010287761232036No Hit
TAAGTC2484830.8710804874892891No Hit
ACCGCT2434870.8535665403963432No Hit
TTCTAC2434450.8534193054528075No Hit
GGCCGT2390700.838082332167852No Hit
CAAGCT2389890.8377983790624618No Hit
TATAAG2293960.8041692168401579No Hit
CAGTCC2198670.770764412627051No Hit
GACCGA2186790.7665997670813305No Hit
AGTGCA2179720.7641213121984817No Hit
TACAGT2153730.7550102736687446No Hit
TATGGT2127000.7456398211908734No Hit
GAGTGA2083750.7304781276006029No Hit
ACGCTA2077630.728332704137656No Hit
ATCCAC2058120.7214932904510392No Hit
GTAAGA2029360.711411202412746No Hit
TCGGAC1997790.700344042490322No Hit
GTTACA1982490.6949804838329546No Hit
AAGTTG1980670.6943424657443005No Hit
GACTAA1978200.6934765840525556No Hit
ACCTGC1976260.692796498837177No Hit
CAGGAC1971210.6910261739208564No Hit
TCTCTG1952120.6843339951777752No Hit
TTACGT1927360.6756541446969637No Hit
AAGCAT1913760.670886537001526No Hit
TAATGA1884580.6606572140196972No Hit
TTCGTT1877380.6581331864162303No Hit
AGCCTA1872850.6565451523823823No Hit
TGCGGA1867260.6545855253958016No Hit
ATTGCT1866430.6542945611026242No Hit
AAGCGC1865200.6538633730536986No Hit
CCGCTC1861700.6526364151909022No Hit
AGTAGG1859640.6519142628487992No Hit
ATACCT1821170.6384282431397192No Hit
TTCACA1809470.6343266982840854No Hit
GGAGGC1803440.6322128251661818No Hit
AGATGC1786470.6262638323285659No Hit
GGCAGG1783060.6250684248108127No Hit
ACCAGG1767450.6195961927427406No Hit
TTCCAT1758930.6166094267453046No Hit
GGATTC1755160.6152878178473782No Hit
TGTGAT1729410.6062609135710899No Hit
TCCAGA1719290.6027132525506613No Hit
AACTAG1712140.6002067529166629No Hit
CAACGG1709920.5994285110722606No Hit
CGTACT1708960.599091974058465No Hit
CATGAG1706170.5981139133621215No Hit
CGTCTC1704330.59746888408568No Hit
GATCCT1694130.5938931783141017No Hit
ATCCGG1688410.5918879786069029No Hit
TACTCG1686110.591081692011351No Hit
CTCCGC1668470.5848978243828569No Hit
TCCTCC1660390.5820653045167439No Hit
TATTGG1654510.5800040153072459No Hit
GGATCG1654210.5798988474904347No Hit
TCTTCA1641900.575583461407285No Hit
CATAAC1639950.5748998705980126No Hit
TTAGTA1631430.5719131046005766No Hit
GCACAT1630960.5717483416875726No Hit
CCGTCG1623430.5691086294856134No Hit
GTACGC1613260.5655434404957163No Hit
TGCGAC1603040.5619607235363506No Hit
AGGCTT1593490.558612881367863No Hit
CGAATT1591720.5579923912486775No Hit
AATTGC1589480.5572071382164877No Hit
TGACAC1588970.5570283529279088No Hit
TTGAAT1558770.5464414593689223No Hit
GGTACC1558580.5463748530849419No Hit
AATGTA1557830.5461119335429141No Hit
CCATGA1547300.5424205431728436No Hit
CATTAA1532090.5370885348605196No Hit
CCTTAT1531750.5369693446681337No Hit
ACACGA1530480.5365241342436332No Hit
GGTGGT1527960.5356407245824198No Hit
AGACGG1527420.5354514225121598No Hit
CGTTGC1524620.5344698562219227No Hit
GTCTGC1519640.5327240704628579No Hit
GCTGTC1517940.5321281195009282No Hit
TGGCAT1507670.528527874572094No Hit
ACAGCC1502910.5268592118786909No Hit
AGTTCC1499610.5257023658937685No Hit
TACGAA1493890.5236971661865698No Hit
ACTAGC1478820.5184142361887576No Hit
GTACTT1476180.5174887594008197No Hit
ACGTAA1468350.5147438793820494No Hit
GAGCCA1455910.5103829205782814No Hit
GCGCAG1455290.5101655737568717No Hit
AACATA1448810.5078939489137515No Hit
CCTGCT1438410.5042481312642991No Hit
AGAACG1433950.5026846363877071No Hit
CGAAGC1426020.4999047004299996No Hit
TCGTGG1425540.4997364319231018No Hit
TCAAGC1423180.4989091117641876No Hit
AGGTCA1415480.4962098044660355No Hit
GGTAAT1412700.4952352493635857No Hit
CCAGCG1409690.49418006560158073No Hit
TCAATG1407540.49342636291443437No Hit
AGTCAA1405210.4926095595372013No Hit
ATCAGC1401580.4913370289537867No Hit
CGCTTG1368170.4796248397549211No Hit
GGACTA1365660.4787449356876014No Hit
GTCTCA1364430.4783137476386758No Hit
GTGGTG1359340.4765294003467804No Hit
GCTCAA1335590.468203614849233No Hit
TTCTGT1319440.4625420807109008No Hit
ATTCGA1310720.4594852028355908No Hit
GAGAAG1306380.45796377508572317No Hit
ACTAAT1295380.4541076218026486No Hit
CCACTT1294680.45386223023008926No Hit
AGGACT1289900.4521865563488987No Hit
GAACCG1282960.449753674186668No Hit
CTATGC1280280.44881417502315535No Hit
CTAGTT1279380.44849867157272194No Hit
CGTCAG1278500.44819017931007593No Hit
AACCTT1270670.44544529929130566No Hit
TCTCGC1262140.442455027699976No Hit
AAGAGG1255950.44028506507977316No Hit
GAATAC1254700.4398468658430601No Hit
ATTCAT1249360.4379748787038221No Hit
CGAGCC1246670.4370318739464156No Hit
CTGTAT1241380.4351774147766462No Hit
CTGTTC1216860.4265816985492836No Hit
GAGATA1211840.4248218904146441No Hit
GACACG1203990.4220699992080863No Hit
CCGACT1197730.4198754974306275No Hit
AACTCT1186510.4159422210818915No Hit
TACGGC1176770.41252777262942364No Hit
ATGTGA1169120.4098459933007399No Hit
GCTTCT1167540.40929210946553474No Hit
GAGCAC1142390.40047554082286874No Hit
AGTTAG1135490.39805668103621283No Hit
GCGTAT1130050.39614963795803787No Hit
GACCAT1126640.3949542304402847No Hit
CGACAA1120910.39294552513919223No Hit
CTTCAC1116500.39139955823206873No Hit
CTGGAG1113830.3904635646624497No Hit
GATTCA1103650.38689487007865886No Hit
GAGTCG1103150.38671959038397363No Hit
TTAGCG1058920.3712143485921202No Hit
CTGAGG1057300.3706464423813401No Hit
CCGGAA1050620.36830470566034573No Hit
CTACCG1049290.3678384616724831No Hit
CCTCGG1042660.36551425292095724No Hit
CACATT1041860.36523380540946093No Hit
AGGTGT1039450.3643889572810782No Hit
AGAGGT1021990.35826819034267077No Hit
GACTTG1020830.3578615414510011No Hit
AACAAT1019810.3575039708738432No Hit
CTCGTC1014630.3556880732369045No Hit
ATGCCG1005230.3523928149768226No Hit
GTCGAG990600.34726413111033344No Hit
CAGCCT986950.3459845893391314No Hit
GCCTGG978900.34316258625469953No Hit
TCATGT975460.3419566619552653No Hit
GGCGTT973650.3413221494605049No Hit
CCTGAC966110.33867893166465196No Hit
GAAGAT913870.3203657091639414No Hit
GCGGCT905710.31750514454667883No Hit
TGCATA888110.3113352992937596No Hit
CATACG880680.3087306430307374No Hit
ACGAAC867450.3040927423093668No Hit
ATGGCC821410.2879529880227528No Hit
CAAGGA814740.2856147568956522No Hit
CAGTAG798400.27988661647333957No Hit
AAGGCG785180.2752522213458627No Hit
TCCACG762230.2672068833598117No Hit
GTTGGC733090.25699158275355777No Hit
ATTGAA712630.24981913764703909No Hit
CGTGAA710210.24897078392476266No Hit
GCCATA621810.21798133390441796No Hit
GGCCAG603330.21150299638885267No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)