FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002208484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13561849
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC1589311.1718977257452137No Hit
CTATTA1477091.089150896754565No Hit
ACCAGG1281790.945143984422773No Hit
GGAGGC1277760.9421724132159266No Hit
GTCTGC1276540.9412728308654669No Hit
TACGAA1257690.9273735461882815No Hit
AATCGG1235330.9108861188470686No Hit
GCATAA1230240.9071329433029376No Hit
AGACGG1214360.8954236254953141No Hit
GGCGTT1198980.8840829889788626No Hit
TTCTAC1198570.8837806703201017No Hit
AGCCTA1186960.8752198907390873No Hit
ACTAGC1184610.8734870886705788No Hit
ATACCT1165030.8590495293082824No Hit
TGCGAC1152870.8500832003069787No Hit
TACTCG1109380.8180153016008362No Hit
AACTAG1094460.8070138518722632No Hit
ACCGCT1072300.7906738970475191No Hit
CCATGA1048930.7734417334981387No Hit
CCTTAT1037300.7648662066654776No Hit
AGGACT1034560.7628458331898549No Hit
TCAATG1030170.7596088114533645No Hit
CAAGCT1019230.7515420648025206No Hit
ATTGAA1018620.7510922736272908No Hit
GGACTA996450.7347449451767233No Hit
TGGCAT995650.734155055110848No Hit
TTGAAT978330.7213839351846493No Hit
GTTACA973380.7177339904020462No Hit
GCTCAA973250.7176381332663415No Hit
TGTGAT968650.7142462653875589No Hit
AGTTAG950670.7009884861570129No Hit
TAGAGC928150.684383080802625No Hit
ATTCAT925860.6826945204890572No Hit
TATGGT913180.6733447629449347No Hit
AGTGCA913050.6732489058092299No Hit
CCTGCT902400.6653959943072659No Hit
TTAGTA901230.6645332800859234No Hit
TCTCTG900700.6641424779172811No Hit
GAACCG891570.6574103575404799No Hit
CGTCTC891500.6573587421597159No Hit
GTAAGA879930.6488274570819953No Hit
CTTCAC874990.6451848859252157No Hit
TGATAA869340.6410187873349719No Hit
GGTGGT863070.6363955239436747No Hit
GATCCT862750.6361595679173245No Hit
TGCGGA862020.6356212932322134No Hit
TCGTGG859820.6339990955510565No Hit
ATCCAC855870.6310865133507976No Hit
GTCTCA854790.630290161761866No Hit
CAAGGA848490.6256447774930984No Hit
CAACGG846870.6244502501097011No Hit
CATAAC842850.6214860525286781No Hit
ATGCCG842130.6209551514693904No Hit
CGTTGC830750.612563965282315No Hit
GACTAA822870.6067535481334441No Hit
TCGGAC822270.6063111305840376No Hit
CTTAGC819510.604276009856768No Hit
CTGTTC818430.6034796582678364No Hit
TAAGTC815990.601680493566917No Hit
CCACTT812030.5987605377408346No Hit
AACCTT810910.5979346916486092No Hit
AGTCAA809710.5970498565497964No Hit
GCACAT802220.5915270108080396No Hit
AAGCGC798430.5887324066209556No Hit
CTATGC797430.5879950440386116No Hit
CTGAGG794640.5859378024338717No Hit
AACTCT794000.5854658903811715No Hit
AGGTGT786090.57963335235483No Hit
GTACGC782770.5771853085814478No Hit
GCGCAG781740.5764258251216334No Hit
TCCTCC777560.5733436495274353No Hit
TCAAGC760450.5607273757435287No Hit
CCTGAC758530.5593116395854282No Hit
AGTAGG756630.5579106506789745No Hit
TATTCT753410.5555363431638267No Hit
TTCCAT743780.5484355414958535No Hit
CCGACT740190.5457884098252385No Hit
CGTCAG739600.5453533659016555No Hit
TTCGTT737440.5437606627237923No Hit
TCATGT736450.5430306737672718No Hit
CACATT733640.540958684910885No Hit
CAGTAG727920.5367409709398769No Hit
TTACGT727580.53649026766188No Hit
GGCCAG726000.5353252347817764No Hit
ACTAAT721250.5318227625156422No Hit
CATGAG720920.5315794328634686No Hit
CTGTAT715370.5274870705314593No Hit
CGTACT712480.5253560926684849No Hit
TCTTCA711960.524972664125666No Hit
ATGTGA708510.5224287632165792No Hit
CAGGAC708320.5222886643259338No Hit
GAAGAT704540.5195014337646732No Hit
GCTGTC702140.5177317635670475No Hit
AACATA698640.5151509945288434No Hit
GCGTAT696030.5132264781889254No Hit
GGATTC688920.5079838302284593No Hit
CTGGAG687380.5068482918516495No Hit
TTCACA685010.5051007425314941No Hit
CGACAA681460.5024831053641727No Hit
AAGGCG679480.5010231274511315No Hit
ATCAGC677850.4998212264419106No Hit
CACCTA674730.49752065518499733No Hit
AGGTCA671470.4951168531665557No Hit
GGCCGT662040.48816352401505136No Hit
AAGAGG660800.4872491944129447No Hit
GGTAAT660150.48676990873442116No Hit
GTCGAG658240.48536154620214395No Hit
CGAATT657130.4845430737357421No Hit
GCCATA656130.48380571115339804No Hit
CCGTCG653710.48202129370412544No Hit
ACACGA653180.48163049153548315No Hit
CGAGAG647550.47747914019688614No Hit
TAATGA647430.47739065668700487No Hit
GACTTG647080.47713257978318446No Hit
CCAGCG639480.4715286241573697No Hit
GAGAAG639060.4712189318727852No Hit
ATGGCC625050.4608884820941452No Hit
GAGCAC619760.4569878340335451No Hit
CATACG618150.4558006802759712No Hit
AGTTCC616290.4544291858728113No Hit
AGGCTT611830.45114054875555687No Hit
ATTCGA609410.4493561313062843No Hit
CTACCG608690.44882523024699655No Hit
CTCCGC604070.4454186151165671No Hit
CAGCCT598770.44151059343014365No Hit
ACGAAC593260.4374477256014279No Hit
TGTGTG592550.43692419816796374No Hit
GCTTCT591220.4359435059334461No Hit
AAGCAT589880.4349554400731051No Hit
GTGGTG584400.4309146931218597No Hit
GGATCG581720.4289385614011777No Hit
CTCGTC580400.42796524279248355No Hit
TCTCGC579340.42718363845519886No Hit
GACACG578020.4262103198465047No Hit
GAGTCG572440.42209583663702493No Hit
ACGTAA567980.4188071995197705No Hit
TATAAG562260.41458948554876257No Hit
CCGCTC560760.4134834416752465No Hit
GATTCA560570.4133433427846011No Hit
CGTGAA554610.4089486617938306No Hit
AAGTTG552560.40743706850002537No Hit
TACAGT552290.4072379806027924No Hit
CGAGCC550350.40580749719304504No Hit
TGACAC547230.4035069259361316No Hit
GCCTGG541910.3995841569980612No Hit
CTAGTT540540.3985739702602499No Hit
AGATGC538320.3969370253274461No Hit
AATTGC527900.38925370721942115No Hit
ATTGCT526070.38790433369373156No Hit
ATCCGG523860.38627476238675124No Hit
TACGGC519440.3830156197727906No Hit
CCGGAA516770.381046861677932No Hit
TGCATA513240.37844397176225747No Hit
TCCAGA510650.3765342026739864No Hit
CAGTCC510430.3763719829058707No Hit
CATTAA510100.3761286532536972No Hit
ACCTGC501270.3696177416515993No Hit
TTAGCG495810.3655917419520008No Hit
ACAGCC494540.36465529147242387No Hit
AGAGGT494340.364507818955955No Hit
GAGCCA479780.35377181975702576No Hit
CCTCGG468460.3454248753248912No Hit
GGCAGG464320.34237219423398685No Hit
TTCTGT461350.340182227364425No Hit
GAGTGA454500.3351312936753683No Hit
ACGCTA454120.3348510958940776No Hit
GACCAT449560.33148872251858874No Hit
GTACTT446150.32897431611279554No Hit
GTTGGC434570.3204356574092515No Hit
AATGTA431540.31820144878474904No Hit
AGAACG429950.317029042278822No Hit
CGAAGC402260.29661147237371543No Hit
TATTGG395950.29195871447912447No Hit
GAGATA395510.29163427494289307No Hit
TCCACG356930.26318682651605985No Hit
CGCTTG338620.24968571763334038No Hit
GCGGCT254150.18740070030273895No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)