FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24315806
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGG4832871.9875425885533056No Hit
ACTAGC4391341.805961110234224No Hit
AGGACT3867511.590533334572582No Hit
TCAATG3830651.5753744704164856No Hit
GGTACC3511601.4441635206334513No Hit
TGCGGA3237871.331590653421071No Hit
CTATTA3184831.3097776812333508No Hit
GACTAA3109211.2786785681708432No Hit
CGTTGC3086141.269190912281501No Hit
TCCTCC2898951.1922080641702768No Hit
CATGAG2763431.1364747687162828No Hit
GCTGTC2717971.1177791104271848No Hit
AACATA2674681.099975875773972No Hit
TTCGTT2653151.091121552787516No Hit
GCGTAT2598101.0684819577849898No Hit
TAGAGC2547681.0477464740424398No Hit
TACGAA2363920.9721742310330983No Hit
GTCTCA2340370.962489172680519No Hit
CTTCAC2297620.9449080157984482No Hit
GAGCAC2284480.9395041233673274No Hit
CAAGGA2262180.9303331339294285No Hit
GCTTCT2212580.9099348793949089No Hit
GACACG2209750.9087710273720724No Hit
TTCTAC2198490.9041402945886309No Hit
GTCTGC2183770.8980866190493542No Hit
AACTCT2164420.8901288322501011No Hit
TGCGAC2122720.8729794932563617No Hit
TGTGTG2121650.8725394502653953No Hit
CCGCTC2078020.8545963888673893No Hit
CCTGAC1984330.8160658955742615No Hit
AAGTTG1978460.8136518279509222No Hit
AGTTAG1928650.7931672098387361No Hit
ATACCT1903390.7827789052108739No Hit
TGGCAT1858810.764445151437711No Hit
GCTCAA1832030.7534317390095974No Hit
GATCCT1831360.7531561980713286No Hit
TGTGAT1799970.7402468994858735No Hit
CATTAA1764400.725618554449727No Hit
ATTGAA1723730.7088928082416844No Hit
GTTGGC1706130.701654717922984No Hit
AGTGCA1677130.6897283191023978No Hit
GAACCG1643290.675811445444169No Hit
AGTTCC1605450.660249551258963No Hit
ATTCAT1590710.6541876506170513No Hit
CTGTTC1540690.633616668927199No Hit
GAAGAT1540190.6334110413613269No Hit
AACCTT1524260.6268597471126394No Hit
AATGTA1518300.6244086665274431No Hit
CAGGAC1512000.6218177591974537No Hit
TCGGAC1505270.6190500121608142No Hit
CCACTT1498530.6162781525728573No Hit
TGATAA1482660.6097515336320745No Hit
GCATAA1482570.6097145206702176No Hit
TACAGT1478320.607966686360304No Hit
TAAGTC1464290.6021967768619308No Hit
CTGAGG1445110.5943089034350744No Hit
GCGCAG1424430.5858041473106012No Hit
CTATGC1422020.5848130224430973No Hit
TGACAC1419080.5836039323557689No Hit
TACTCG1416630.5825963572829953No Hit
ACACGA1415380.5820822883683148No Hit
AACTAG1354530.5570574136016713No Hit
TTCCAT1353750.5567366345989107No Hit
CGAGAG1352530.5562349033381825No Hit
TCAAGC1337050.54986867389878No Hit
TCATGT1337020.5498563362448278No Hit
CGTACT1330610.5472201908503465No Hit
TCTTCA1329310.5466855591790788No Hit
AATCGG1327130.5457890229918761No Hit
CCGTCG1307340.5376502839346555No Hit
GCCATA1300080.5346645716781916No Hit
ATGTGA1286380.529030376373294No Hit
GACTTG1284890.528417606226995No Hit
TTAGCG1282840.527574533206919No Hit
CGACAA1262170.5190738896337633No Hit
TTCACA1231960.5066498721037667No Hit
GGATCG1227460.5047992240109169No Hit
ACTAAT1216890.5004522572683793No Hit
CGAGCC1214920.4996420846588429No Hit
AGGTCA1212820.49877844888217976No Hit
GTACTT1209880.49756935879485137No Hit
GGCAGG1208570.49703061457226627No Hit
GGAGGC1208410.4969648137511872No Hit
CTGTAT1207690.4966687100563313No Hit
CATACG1204030.495163516274147No Hit
CCAGCG1176260.4837429612656064No Hit
TAATGA1167880.48029664326158883No Hit
GTTACA1158990.4766405851403815No Hit
CGTCTC1147390.4718700256121471No Hit
GGACTA1144630.47073496144853266No Hit
ACCGCT1106400.45501267776194626No Hit
AGACGG1106120.45489752632505787No Hit
ACGAAC1101890.45315791711777925No Hit
CTTAGC1093820.44983908820460233No Hit
CTACCG1085630.44647090867561623No Hit
ATTCGA1082640.44524125583170054No Hit
GGCGTT1078970.44373194949819883No Hit
GTGGTG1075820.44243649583320416No Hit
TATGGT1070930.4404254582389743No Hit
AAGCAT1062700.4370408285047183No Hit
ATCAGC1060310.4360579287398493No Hit
AGCCTA1048770.4313120445195195No Hit
AAGCGC1044500.4295559851069711No Hit
CAACGG1030620.42384776387835965No Hit
AGGTGT1009400.4151209299827445No Hit
GAGCCA1000620.4115101099260292No Hit
CAGTCC992060.4079897659982975No Hit
GTAAGA989620.4069863034768414No Hit
ATCCGG988470.40651336007533534No Hit
CCTTAT985230.4051808934484836No Hit
GATTCA977680.40207591720381386No Hit
TGCATA972520.399953840724013No Hit
TTGAAT950540.39091445292827226No Hit
GCCTGG939300.3862919452474658No Hit
CCGGAA936260.385041729646963No Hit
GCGGCT935070.3845523360401872No Hit
AATTGC931800.38320753175938316No Hit
ATTGCT911470.37484671493102056No Hit
AGTAGG896780.36880537704569616No Hit
TATAAG889060.36563048742862975No Hit
GGTAAT887920.3651616565784412No Hit
GTCGAG887850.3651328687192191No Hit
GGCCGT884040.36356598666727313No Hit
ATCCAC875070.3598770281355263No Hit
AGTCAA856950.3524250851483187No Hit
TATTGG835740.34370236380402114No Hit
CTAGTT833920.34295387946424644No Hit
CACATT830890.34170777641506106No Hit
TCTCTG799840.32893830457439904No Hit
ATGCCG796040.32737553507377054No Hit
CGAAGC786570.32348094897615154No Hit
TATTCT775090.3187597400637265No Hit
CTGGAG774880.31867337648606014No Hit
GGTGGT770990.3170735940235746No Hit
TCCACG769380.3164114732614662No Hit
CCATGA761320.3130967568996068No Hit
AGAACG755100.31053874998015696No Hit
CGAATT740880.30469070200675236No Hit
CTCCGC733580.30168853954501856No Hit
TCTCGC724910.2981229575527951No Hit
TTCTGT699460.2876565144499015No Hit
GAGTCG698700.28734396054977573No Hit
GGATTC698700.28734396054977573No Hit
TTACGT698070.2870848698167768No Hit
CAAGCT690150.2838277291733616No Hit
GTACGC689260.2834617121061091No Hit
GACCAT686520.28233487304512955No Hit
GGCCAG679610.2794931000847761No Hit
CAGTAG676510.2782182091763687No Hit
TCCAGA671520.27616604606896433No Hit
TTAGTA667630.27456626360647884No Hit
CCTGCT652170.26820825926971126No Hit
AAGGCG647140.26613964595703715No Hit
TACGGC646030.26568315276080096No Hit
CGTCAG644780.2651690838461205No Hit
ACGTAA644560.26507860771713676No Hit
CCGACT640140.26326086003482674No Hit
TCGTGG626440.2576266647299292No Hit
AGAGGT620280.255093333118384No Hit
GAGAAG608030.25005545775451576No Hit
ACAGCC596710.24540004966316972No Hit
GCACAT594170.24435546162853905No Hit
ATGGCC582960.23974529160168492No Hit
CGCTTG577800.2376232151218841No Hit
CCTCGG571200.2349089312523714No Hit
CAGCCT568550.23381910515324886No Hit
CATAAC567620.23343663788072663No Hit
GAGTGA531630.21863556568924758No Hit
CGTGAA521420.21443665079413776No Hit
CTCGTC508220.20900808305511237No Hit
AAGAGG482670.1985005144390443No Hit
CACCTA452710.18617931069198365No Hit
GAGATA449860.18500723356651225No Hit
AGGCTT421930.17352087773689262No Hit
ACGCTA382010.15710357287765825No Hit
AGATGC360950.1484425398031223No Hit
ACCTGC332030.13654904139307575No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)