FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002208488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34749153
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC4101071.18019279491503No Hit
CTATTA3851331.1083234172643No Hit
TACGAA3260950.9384257509816138No Hit
ACCAGG3237680.9317291848811394No Hit
TTCTAC3167290.9114725760366016No Hit
GTCTGC3161570.909826492749334No Hit
ACTAGC3119140.8976161231901105No Hit
AATCGG3073350.8844388235880166No Hit
GCATAA2984990.859010865674913No Hit
GGAGGC2975950.856409363416714No Hit
TGCGAC2967020.8538395166063473No Hit
AGCCTA2962530.852547398781202No Hit
GGCGTT2934870.8445874925354295No Hit
ATACCT2904700.8359052665254891No Hit
AGACGG2900400.8346678262920537No Hit
AACTAG2768290.7966496334457418No Hit
TACTCG2741030.7888048379193588No Hit
AGGACT2723700.7838176660018159No Hit
TCAATG2715870.7815643736697696No Hit
CCTTAT2656860.7645826647918584No Hit
ACCGCT2634930.7582717196013382No Hit
CCATGA2604270.7494484829601458No Hit
TGTGAT2562340.7373820018001589No Hit
ATTGAA2560880.7369618476743879No Hit
CAAGCT2537050.7301041265667685No Hit
TTGAAT2529750.7280033559379131No Hit
GGACTA2529080.7278105454829359No Hit
TGGCAT2527340.7273098138535924No Hit
GCTCAA2500370.7195484735987666No Hit
AGTTAG2490190.7166189057903081No Hit
TAGAGC2430880.6995508638728547No Hit
AGTGCA2394250.6890095997447765No Hit
GTTACA2386120.6866699743731883No Hit
ATTCAT2367150.6812108485061492No Hit
TATGGT2314760.6661342220341313No Hit
TTAGTA2293550.660030476138512No Hit
GAACCG2289160.658767135993214No Hit
CCTGCT2263670.6514317054001287No Hit
TCTCTG2253510.6485078931276397No Hit
CTTCAC2241920.6451725600333338No Hit
GTAAGA2229520.6416041277322645No Hit
GATCCT2223720.6399350223011191No Hit
TGATAA2217550.6381594394545387No Hit
GTCTCA2214310.6372270426274851No Hit
CAAGGA2208880.6356644146117749No Hit
TGCGGA2187560.6295290132683234No Hit
CGTCTC2170700.624677096446063No Hit
TCGGAC2168270.6239777988257729No Hit
CGTTGC2158110.6210539865532837No Hit
AACCTT2156050.6204611663484287No Hit
CCACTT2139580.6157214824775729No Hit
CTGTTC2138030.6152754284399393No Hit
GGTGGT2135560.61456461974771No Hit
TAAGTC2133610.6140034549906871No Hit
TCGTGG2131530.6134048792498626No Hit
ATCCAC2124460.6113702972846562No Hit
CAACGG2115990.608932827801587No Hit
AACTCT2110560.6073701997858768No Hit
GACTAA2108760.6068522015486248No Hit
CATAAC2100300.6044176098335404No Hit
ATGCCG2092610.6022046062532805No Hit
CTTAGC2068930.595390051665432No Hit
CTGAGG2064770.5941929001837829No Hit
GCACAT2043570.5880920320561482No Hit
AGTCAA2025330.5828429832519947No Hit
CTATGC2016920.5804227803768339No Hit
TCCTCC2000580.5757205074897797No Hit
TTCCAT1978050.5692368962201755No Hit
AGGTGT1960310.5641317358152586No Hit
TTCGTT1943490.5592913300649371No Hit
GCGCAG1942620.5590409642502653No Hit
AAGCGC1941950.5588481537952882No Hit
TCAAGC1939440.5581258340311201No Hit
GTACGC1938220.5577747463369827No Hit
TCATGT1937150.5574668251626163No Hit
TATTCT1928690.5550322334475318No Hit
CCTGAC1921420.5529400961226306No Hit
TCTTCA1903510.5477860136619733No Hit
CACATT1889350.5437110941955909No Hit
CGTACT1884800.5424017097625372No Hit
CATGAG1868870.537817425362857No Hit
AGTAGG1867970.537558426244231No Hit
AACATA1864530.5365684740574828No Hit
CCGACT1859220.5350403792575894No Hit
GAAGAT1854260.5336130063371617No Hit
ACTAAT1850820.5326230541504133No Hit
TTACGT1845690.5311467591742453No Hit
ATGTGA1836810.5285913012038019No Hit
CGTCAG1827390.5258804437621831No Hit
CTGTAT1824640.5250890575663816No Hit
GCTGTC1823610.524792647463954No Hit
CAGTAG1819290.5235494516945491No Hit
CAGGAC1810400.5209911159561212No Hit
CGACAA1786690.5141679280643185No Hit
TTCACA1778440.5117937694769136No Hit
GCGTAT1773360.510331863340669No Hit
GGCCAG1747920.5030108215875075No Hit
AGGTCA1714980.49353145384579594No Hit
CTGGAG1714770.4934710207181165No Hit
GCCATA1713270.4930393555204065No Hit
ACACGA1707740.49144794982484896No Hit
TAATGA1703340.4901817319115663No Hit
CGAATT1702650.4899831659206197No Hit
GACTTG1699900.4891917797248181No Hit
CCGTCG1693770.48742770795017654No Hit
GGATTC1691340.4867284103298863No Hit
ATCAGC1686080.4852147043699166No Hit
CACCTA1678140.48292975659003834No Hit
GGTAAT1674960.48201462637089315No Hit
AAGGCG1673150.48149375036565634No Hit
AAGAGG1658220.4771972427644495No Hit
CGAGAG1656000.4765583782718388No Hit
CCAGCG1630370.46918265892696726No Hit
GTCGAG1627010.46821572888409685No Hit
GAGCAC1604820.46182996172597357No Hit
GAGAAG1592150.4581838296893165No Hit
GGCCGT1580940.45495785177843034No Hit
ATTCGA1568160.4512800642939412No Hit
AGTTCC1565140.4504109783625518No Hit
ACGAAC1564930.45035054523487233No Hit
AAGCAT1550480.44619217049693266No Hit
ATGGCC1545300.4447014866808408No Hit
CTACCG1540220.4432395805445963No Hit
GCTTCT1539550.4430467700896191No Hit
AGGCTT1522270.438073987012No Hit
TGTGTG1514470.435829327983908No Hit
CAGCCT1513580.4355732066332667No Hit
CATACG1513080.43542931823403No Hit
GTGGTG1486680.4278320107543341No Hit
GACACG1484340.42715861304590647No Hit
GGATCG1466870.42213115237657733No Hit
CTCCGC1461560.420603057576684No Hit
CGAGCC1457190.4193454729673555No Hit
TACAGT1456530.4191555402803631No Hit
CTCGTC1455550.41887351901785924No Hit
AAGTTG1454570.41859149775535537No Hit
TCTCGC1425490.41022294845575086No Hit
GATTCA1424060.409811427633934No Hit
TGACAC1413180.40668041606654415No Hit
CCGCTC1411830.40629191738860515No Hit
TATAAG1404390.4041508580079635No Hit
GAGTCG1403460.4038832255853833No Hit
AATTGC1386720.3990658419789397No Hit
ACGTAA1386570.3990226754591687No Hit
CGTGAA1380330.39722694823669513No Hit
TGCATA1363330.39233474266264845No Hit
CTAGTT1361890.39192034407284687No Hit
CAGTCC1355230.39000375059501446No Hit
ATTGCT1351780.38901092064028153No Hit
ATCCGG1347910.3878972244301897No Hit
AGATGC1347040.3876468586155179No Hit
GCCTGG1340470.3857561650495481No Hit
CATTAA1321500.380297039182509No Hit
CCGGAA1303600.37514583448983635No Hit
TCCAGA1277490.36763198228169763No Hit
TTAGCG1259110.3623426447257578No Hit
ACAGCC1257020.3617411912169485No Hit
AGAGGT1243090.35773246041421497No Hit
ACCTGC1240280.3569238076105049No Hit
GAGCCA1230560.3541266171293441No Hit
TTCTGT1183830.3406788073366853No Hit
GTACTT1179260.33936366736766216No Hit
TACGGC1174130.33788737239149397No Hit
CCTCGG1168440.3362499224081807No Hit
GGCAGG1159250.33360525363021076No Hit
ACGCTA1145870.3297548000666376No Hit
GAGTGA1143150.32897204717479017No Hit
AATGTA1135790.3268540099380264No Hit
GACCAT1126870.32428704089564425No Hit
AGAACG1102610.3173055757646812No Hit
GTTGGC1100840.31679621083138343No Hit
TATTGG1034390.2976734425728305No Hit
CGAAGC1033540.29742883229412814No Hit
GAGATA1002370.2884588294857144No Hit
TCCACG924430.26602950581270285No Hit
CGCTTG847330.24384191465040891No Hit
GCGGCT621560.17887054685908457No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)