FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002208490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32939701
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3733861.1335439869354005No Hit
CTATTA3506721.064587684022997No Hit
GTCTGC3160390.9594470818056302No Hit
AATCGG3058270.9284449789025104No Hit
GGAGGC3051480.9263836365727789No Hit
GCATAA3010600.9139730806906838No Hit
TACGAA2963220.8995892221365336No Hit
GGCGTT2949500.8954240355733648No Hit
AGACGG2941170.8928951723028693No Hit
AGCCTA2935450.8911586659514609No Hit
ACCAGG2933840.890669894058844No Hit
ATACCT2887630.8766412299856637No Hit
TTCTAC2870610.8714742128351438No Hit
ACTAGC2812610.8538662812998818No Hit
TACTCG2745430.8334714392216249No Hit
AACTAG2740530.8319838725919219No Hit
TGCGAC2711450.8231556200221732No Hit
CCTTAT2634390.7997613578823926No Hit
ACCGCT2598120.7887503289723242No Hit
CCATGA2580260.7833283003995695No Hit
ATTGAA2542440.7718467146984728No Hit
CAAGCT2496920.7580275242935569No Hit
GGACTA2496340.7578514449782043No Hit
TTGAAT2492020.7565399576638537No Hit
AGGACT2465330.7484372733073685No Hit
TCAATG2462710.747641880538017No Hit
GTTACA2379180.7222834232769751No Hit
ATTCAT2341470.7108352319287901No Hit
TGGCAT2341180.7107471922711138No Hit
TGTGAT2325950.7061235923179752No Hit
TATGGT2284310.693482311815763No Hit
TTAGTA2280760.6924045849717944No Hit
GCTCAA2276500.691111312759032No Hit
AGTTAG2272550.6899121519044754No Hit
CCTGCT2238770.6796570497103176No Hit
TCTCTG2236930.6790984532616128No Hit
TAGAGC2215310.6725349449893306No Hit
TGATAA2208140.6703582403495405No Hit
GTAAGA2197600.6671584541705464No Hit
CGTCTC2176490.660749774261764No Hit
AGTGCA2170450.658916120701885No Hit
TCGTGG2150580.6528838862259254No Hit
GGTGGT2132290.6473313160917884No Hit
CAACGG2110290.6406524455094477No Hit
ATCCAC2106010.639353101596156No Hit
ATGCCG2083690.6325770838053448No Hit
CATAAC2083250.632443506393698No Hit
GAACCG2067780.6277470460342066No Hit
CTTCAC2044000.6205277941047492No Hit
CTTAGC2043240.6202970694846319No Hit
GTCTCA2025860.6150207617245828No Hit
AGTCAA2025100.6147900371044656No Hit
GATCCT2021010.613548374346203No Hit
TGCGGA2017420.6124585041011756No Hit
GCACAT2008110.6096321275047396No Hit
CAAGGA1983550.6021760792546356No Hit
CGTTGC1968440.5975889095046735No Hit
TCGGAC1953140.5929440585996819No Hit
AGGTGT1952210.5926617245250647No Hit
CCACTT1948280.5914686353710374No Hit
CTGTTC1947310.5911741578953615No Hit
AAGCGC1942430.5896926629661878No Hit
AACCTT1937130.5880836623258967No Hit
GACTAA1925620.5845893986712265No Hit
GTACGC1924770.584331351398727No Hit
TAAGTC1916810.5819148145880255No Hit
AACTCT1906060.5786512755534726No Hit
TATTCT1904100.5780562489015915No Hit
CTGAGG1874440.5690519170164902No Hit
AGTAGG1862580.5654513986025557No Hit
CACATT1851610.5621210708621793No Hit
CTATGC1845930.5603967079118296No Hit
CCGACT1838850.5582473259244217No Hit
TTACGT1833300.5565624290275131No Hit
CGTCAG1821600.5530104842178136No Hit
TCCTCC1818990.5522181272987269No Hit
ACTAAT1813950.5506880587653179No Hit
CAGTAG1810660.5496892640282315No Hit
CTGTAT1801470.5468993176349719No Hit
TTCCAT1793420.544455458171888No Hit
GCGCAG1791710.5439363277766243No Hit
GGCCAG1766730.5363527738154029No Hit
TTCGTT1764760.5357547113132569No Hit
TCATGT1763510.5353752300301694No Hit
TCAAGC1759340.5341092804697893No Hit
CCTGAC1759000.5340060615607896No Hit
TCTTCA1717600.5214376414649301No Hit
CGTACT1703370.5171176265382615No Hit
CTGGAG1702220.5167685037578209No Hit
CATGAG1699030.5158000675233816No Hit
GGATTC1686810.5120902584999177No Hit
AAGGCG1675340.5086081382463065No Hit
GAAGAT1673340.5080009681933664No Hit
CGAATT1672060.5076123793594848No Hit
ATGTGA1671970.5075850567071024No Hit
AACATA1667710.5062917844943401No Hit
GGTAAT1662520.5047161782069607No Hit
ATCAGC1660480.5040968647529618No Hit
CACCTA1659840.5039025703360209No Hit
GCTGTC1654930.5024119678560531No Hit
AAGAGG1642400.4986080474743836No Hit
CAGGAC1641370.4982953548971194No Hit
GTCGAG1630590.49502270831177253No Hit
GGCCGT1618060.491218787930103No Hit
CGACAA1615970.49058429522478053No Hit
GCGTAT1615780.4905266140697513No Hit
TTCACA1613230.48975247225225266No Hit
GAGAAG1584330.48097886498726866No Hit
AGGTCA1566400.475535585462661No Hit
GCCATA1556550.4725452729519312No Hit
CCGTCG1549830.4705051815740525No Hit
ACACGA1546780.46957924724331895No Hit
TAATGA1545470.4691815508586432No Hit
GACTTG1544240.4688081412760851No Hit
ATGGCC1534250.4657753268616494No Hit
AGGCTT1530330.46458527355788687No Hit
CGAGAG1509330.4582099880020162No Hit
CAGCCT1494250.4536319258028481No Hit
CCAGCG1486050.45114252858579373No Hit
CTCCGC1463150.44419043147963No Hit
GAGCAC1454910.4416888908615169No Hit
CTCGTC1440060.4371806532184369No Hit
AGTTCC1430540.4342905237664422No Hit
ATTCGA1428990.4338199669754137No Hit
TCTCGC1421520.4315521868276825No Hit
CATACG1415140.4296153143588037No Hit
ACGAAC1410080.42807917412486535No Hit
GAGTCG1406070.42686179816872044No Hit
AAGCAT1406030.4268496547676617No Hit
TGTGTG1404510.42638820552742723No Hit
GCTTCT1402660.4258265732284576No Hit
TATAAG1401890.42559281275807576No Hit
CTACCG1400030.4250281446088414No Hit
ACGTAA1379480.4187894723148823No Hit
CGTGAA1376730.4179546134920897No Hit
GTGGTG1362440.4136163834638329No Hit
GACACG1359150.4126175887267465No Hit
GGATCG1355520.4115155750806602No Hit
CTAGTT1341790.40734735266722666No Hit
AGATGC1339050.4065155296946988No Hit
CGAGCC1323220.40170977872567815No Hit
AAGTTG1317280.39990648366844617No Hit
TACAGT1311250.3980758659588319No Hit
GATTCA1299670.3945603513523088No Hit
CCGCTC1295490.39329136594166414No Hit
TGACAC1280230.38865865843773145No Hit
AATTGC1265730.38425667555391596No Hit
TCCAGA1263090.3834552110840351No Hit
TGCATA1239600.3763239988122539No Hit
GCCTGG1235870.3751916266635207No Hit
ATCCGG1230250.37348547881475913No Hit
CAGTCC1229860.37336708065443586No Hit
TACGGC1229510.3732608258951713No Hit
ACAGCC1226220.3722620311580849No Hit
ATTGCT1226020.3722013141527909No Hit
AGAGGT1221460.3708169664320875No Hit
ACCTGC1220240.3704465926997941No Hit
CATTAA1199450.3641350599994821No Hit
CCGGAA1190640.36146047591628105No Hit
TTCTGT1171990.35579861517261496No Hit
CCTCGG1168300.35467838642494054No Hit
TTAGCG1160420.35228613641635664No Hit
GAGTGA1135270.3446509730006353No Hit
ACGCTA1132180.3437128952688429No Hit
GAGCCA1124200.341290286757612No Hit
GACCAT1100130.33398299517047836No Hit
GGCAGG1074380.326165680738875No Hit
GTACTT1069240.324605253702819No Hit
AATGTA1022610.31044908391852133No Hit
GTTGGC1020720.30987530821849296No Hit
AGAACG1009990.3066178408844695No Hit
GAGATA994630.30195477487788974No Hit
CGAAGC943640.2864749743781827No Hit
TATTGG943130.28632014601468303No Hit
TCCACG839960.25499927883376966No Hit
CGCTTG772970.23466211791054206No Hit
GCGGCT576750.17509266401659201No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)