FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25081509
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAAC3312601.3207339319177327No Hit
CTACCG2852851.1374315636272123No Hit
GAAGAT2790771.1126802617816975No Hit
CTATTA2564771.0225740405013113No Hit
ACTTGA2341200.933436660449736No Hit
AGACGG2171110.8656217614338914No Hit
TGATAA2093400.83463877711664No Hit
CTGTAT2050280.8174468290564176No Hit
AATCGG2012500.802383939499015No Hit
AGTGCA1857920.7407528789436074No Hit
ATTCAT1799390.717416962432364No Hit
TCTCTG1756520.7003246893956818No Hit
GCATAA1750170.6977929437977595No Hit
GCTGTC1736940.6925181415520095No Hit
ATGCCG1728020.6889617367120934No Hit
AACTAG1724840.6876938704126614No Hit
AAGGCG1724530.6875702733834714No Hit
ACAGTG1712630.6828257422629556No Hit
TGACCA1705550.6800029455962957No Hit
CCTGAC1701590.6784240932234181No Hit
ACTAGC1688580.6732370049983835No Hit
GTCTCA1677100.6686599279174151No Hit
CTTAGC1660250.6619418313308023No Hit
CGATGT1652570.6588798146076458No Hit
GATCAG1649940.6578312333600024No Hit
ACTAAT1649370.6576039743063306No Hit
AATTGC1647720.656946119150965No Hit
TGTGAT1642910.6550283716980506No Hit
GCCAAT1634630.6517271349183975No Hit
TTGAAT1625110.6479315100219848No Hit
TGTGTG1613960.6434860039720895No Hit
TTCTAC1608740.6414047894805691No Hit
CGTACT1600450.6380995656999744No Hit
GGTACC1593700.6354083400643876No Hit
GAACCG1585850.6322785443252238No Hit
ACAGCC1584040.6315568971547925No Hit
GGTAAT1550670.6182522750126398No Hit
TAGAGC1548390.6173432387979527No Hit
GTTACA1538130.6132525758318608No Hit
GACTAA1535100.612044514546553No Hit
GGCTAC1529120.6096602879834702No Hit
AGCCTA1515080.6040625386614498No Hit
TTCTGT1510570.6022644012367837No Hit
TCTTCA1510120.6020849861944112No Hit
TACGGC1508050.601259676999498No Hit
ACGAAC1506880.6007931978893296No Hit
CTGTTC1490040.5940790883036583No Hit
AACATA1489040.5936803882094973No Hit
TTCCAT1486030.5924803009260727No Hit
CCTGCT1467580.5851242841888022No Hit
TACGAA1466700.5847734281059405No Hit
GAGCAC1466500.5846936880871083No Hit
CCTCGG1464870.5840438069336259No Hit
TTACGT1442360.5750690678140618No Hit
AACCTT1427860.5692879164487272No Hit
TTAGCG1426270.5686539832990113No Hit
TAGCTT1421950.5669315988922358No Hit
TCCAGA1412660.5632276750174801No Hit
GACACG1411990.5629605459543922No Hit
TATTCT1407790.561286005558916No Hit
TGACAC1400690.5584552348903729No Hit
ATGGCC1387470.5531844196455643No Hit
CCTTAT1372490.5472118922350326No Hit
TCAATG1363590.5436634613969996No Hit
TGCATA1358080.5414666238781726No Hit
GGATTC1357680.5413071438405082No Hit
GACCGA1336620.5329105198574774No Hit
CATTAA1321620.5269300184450624No Hit
CTGAGG1309380.5220499292925318No Hit
ATGTGA1303900.5198650527765295No Hit
TACAGT1301370.5188563415383022No Hit
CGTGAA1301350.5188483675364189No Hit
TGGCAT1300030.5183220834121265No Hit
CACATT1298830.5178436432991332No Hit
TACTCG1297660.5173771641889648No Hit
AGTAGG1283620.5117794148669444No Hit
ACCGCT1282560.5113567927671337No Hit
CATGAG1275310.5084662170844665No Hit
TCCTCC1266240.5048500072304263No Hit
GAGATA1256820.5010942523434296No Hit
GAATAC1255740.5006636562417357No Hit
CGTCAG1251070.49880172680200385No Hit
GGCCGT1243150.4956440220562487No Hit
ACCAGG1237820.49351895055437056No Hit
CGCTTG1234740.4922909542643547No Hit
CAACGG1232870.4915453850882736No Hit
CAAGGA1227900.48956384562029337No Hit
GTAAGA1220930.48678490596399127No Hit
GTACGC1219040.486031362786027No Hit
CAGCCT1216420.48498676853932515No Hit
GTCTGC1213520.4838305382662582No Hit
AGGTGT1212540.4834398121739804No Hit
TGCGGA1211030.48283777503179737No Hit
TGCGAC1209150.48208821885477465No Hit
ACGCTA1208590.4818649468020445No Hit
ATTGAA1205540.48064891151485345No Hit
GTGGTG1202850.4795764082615604No Hit
TATGGT1198640.4778978808651425No Hit
GCACAT1185420.47262706562033413No Hit
GTACTT1184840.4723958195657207No Hit
GAGCCA1183480.47185358743766176No Hit
CATACG1183380.4718137174282456No Hit
AGTCAA1183170.4717299904084718No Hit
GCGTAT1182540.4714788093491504No Hit
GGACTA1179430.4702388520563097No Hit
GAGTCG1174260.46817757256949727No Hit
CCGTCG1171200.4669575502813646No Hit
TCGTGG1166710.4651673868585817No Hit
CTTCAC1163240.4637838975318431No Hit
AACTCT1160210.46257583624653525No Hit
CACCTA1154270.4602075576872189No Hit
CCACTT1150730.45879615935388895No Hit
CCGCTC1148540.4579230061476764No Hit
GCTTCT1148330.45783927912790257No Hit
AGAACG1144070.4561408167267767No Hit
CCAGCG1142840.45565041561095865No Hit
CGTTGC1135110.45256846388309413No Hit
TTCACA1134320.4522534908087069No Hit
AAGCGC1131260.45103346852057424No Hit
TAAGTC1129800.4504513663830992No Hit
GGCCAG1127920.4497018102060765No Hit
TTAGGC1119480.44633678141135763No Hit
AAGCAT1113970.44413994389253053No Hit
CCGGAA1112960.443737256797428No Hit
CGTCTC1112520.44356182875599715No Hit
AAGTTG1112200.44343424472586557No Hit
GAGAAG1111470.443143193657128No Hit
CTCCGC1100990.4389648166703207No Hit
TATAAG1092590.43561573587936836No Hit
CGAGAG1089380.4343359085771116No Hit
ATACCT1089230.43427610356298735No Hit
CTATGC1089180.43425616855827937No Hit
AGTTCC1085810.4329125492409568No Hit
AGATGC1085490.43278496521082527No Hit
GGATCG1083540.4320075000272113No Hit
AGGACT1081910.4313576188737288No Hit
CGAAGC1081230.4310865028096994No Hit
CAAGCT1076710.42928437838409167No Hit
CCGACT1075140.4286584192362589No Hit
AGTTAG1074550.42842318618070385No Hit
GGCAGG1043900.4162030282946692No Hit
CAGATC1039110.414293254843638No Hit
CGACAA1033760.4121602093398766No Hit
TCATGT1027810.4097879437796187No Hit
GATCCT1018920.40624349994252734No Hit
CAGGAC1016070.40510720467416855No Hit
CCATGA1013630.40413437644441563No Hit
CTGGAG1013110.40392705239545196No Hit
GCGCAG1010800.40300605517794No Hit
GGCGTT1010200.4027668351214434No Hit
ATTCGA1004530.40050620558755057No Hit
ATCCGG996430.39727673482484643No Hit
GACTTG994660.3965710356581815No Hit
TTAGTA992270.39561814243313675No Hit
TAATGA980230.3908177932994383No Hit
AACAAT977340.38966555002731296No Hit
CTAGTT965010.3847495778663078No Hit
GACCAT964420.3845143448107528No Hit
AGGTCA964400.38450637080886957No Hit
GCCATA954700.3806389798955079No Hit
TCGGAC954290.38047551285690184No Hit
ATCAGC946120.3772181330876065No Hit
TCAAGC939830.3747103094953338No Hit
ACCTGC935380.37293609407631734No Hit
ACGTAA930560.3710143596224613No Hit
GGAGGC925390.3689530801356489No Hit
GTCGAG911410.36337925281927813No Hit
GCGGCT906520.36142960935883084No Hit
CGAATT904920.3607916892081732No Hit
AATGTA901720.35951584890685806No Hit
TTCGTT896610.3574784914256953No Hit
GGTGGT896170.3573030633842645No Hit
TATTGG892950.3560192490810661No Hit
ATCCAC892870.3559873530735332No Hit
GCTCAA890440.35501851184472194No Hit
ATTGCT873730.3483562332712916No Hit
ACACGA864820.3448038154323171No Hit
AGAGGT840640.3351632471555041No Hit
AGGCTT835000.332914578624436No Hit
GTTGGC827130.32977680888338895No Hit
TCTCGC813780.3244541626263396No Hit
GATTCA784220.3126685878429404No Hit
GAGTGA769510.30680370945783203No Hit
GCCTGG767690.30607807528645903No Hit
CTCGTC764730.3048979230077425No Hit
CAGTAG762850.3041483668307198No Hit
CAGTCC737370.29398948843149747No Hit
AAGAGG696720.2777823296038528No Hit
TCCACG671150.267587568196156No Hit
CGAGCC620160.24725785039488654No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)