FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24277655
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGA5258202.1658599234563636No Hit
TGACCA3788801.560612011333055No Hit
ACAGTG3783641.5584866001267421No Hit
GATCAG3715321.530345496712924No Hit
CGATGT3662351.5085270797364903No Hit
GCCAAT3628951.494769573090976No Hit
CCTGAC3507811.44487183791021No Hit
GTCTCA3505111.4437597041394648No Hit
AGACGG3484011.4350685846717897No Hit
GGCTAC3457481.4241408406207272No Hit
TGATAA3325011.369576262616797No Hit
CTGTAT3249941.3386548247761163No Hit
TAGAGC3201761.3188094154892636No Hit
TAGCTT3142961.2945896133708137No Hit
TTAGCG2937041.2097708777886498No Hit
ATTCAT2877461.1852297925808732No Hit
TGACAC2866461.1806988772185782No Hit
ATGCCG2726501.1230491577543218No Hit
TACAGT2683761.1054444920648225No Hit
CTACCG2646821.0902288544754426No Hit
ACTAAT2613221.076388967550614No Hit
TTAGGC2555721.0527046372477078No Hit
CAAGGA2550701.050636892236915No Hit
GCATAA2548951.0499160647929133No Hit
AACTAG2501371.0303177963440042No Hit
GTACTT2462381.0142577608916512No Hit
AACTCT2393590.985923063821444No Hit
CTTCAC2370560.9764369746583843No Hit
CATAAC2354330.9697518149920163No Hit
AGCCTA2353060.9692287002183695No Hit
TTCTGT2340160.9639151722025872No Hit
ACAGCC2304620.9492761965684082No Hit
CAGATC2303090.9486459874316526No Hit
TACGGC2283490.940572720058836No Hit
ATGGCC2215170.9124316166450178No Hit
AGTTCC2203700.9077071076263339No Hit
GTTACA2193740.9036045697164738No Hit
GGCAGG2144420.8832895928375291No Hit
CCTTAT2135520.8796236704080357No Hit
CCTCGG2131110.877807185249152No Hit
GAATAC1991820.8204334397205991No Hit
GTCTGC1951100.8036608148521758No Hit
ATTGAA1918520.7902410673518508No Hit
AGTAGG1841040.7583269471454306No Hit
CAACGG1784870.7351904456999656No Hit
GGCCAG1783610.7346714499402847No Hit
TATGGT1708510.7037376550577064No Hit
GAGTCG1705240.702390737490915No Hit
ATACCT1692570.6971719467963442No Hit
TATAAG1666850.6865778428765051No Hit
CCGACT1655390.6818574528717868No Hit
AATCGG1603880.6606404119343487No Hit
GGCGTT1572930.6478920637104366No Hit
AACAAT1513730.6235075010333576No Hit
GACCAT1497970.6170159350233785No Hit
CGTACT1493680.6152488780320834No Hit
CTAGTT1472310.6064465451873338No Hit
ATCAGC1464520.6032378333080357No Hit
TCTTCA1405590.5789644840080312No Hit
GGTGGT1405210.5788079614773338No Hit
ACGTAA1328550.5472316004161027No Hit
AAGGCG1286920.5300841452767988No Hit
ACTAGC1285950.5296846009221237No Hit
TCTCTG1276640.5258497989200357No Hit
TGTGTG1228660.5060867699124978No Hit
TTGAAT1198390.49361851463825485No Hit
ATGTGA1195390.49238281044853793No Hit
CAGTAG1183600.48752649298295075No Hit
GAGCAC1123690.4628494803143055No Hit
TGCGAC1111760.4579354966531982No Hit
GAGTGA1097040.45187230809565426No Hit
TATTCT1087950.4481281244008122No Hit
CCAGCG1071490.44134822741323243No Hit
TTACGT1070770.44105165840770044No Hit
GGATTC1065250.43877796269862146No Hit
CCTGCT1050680.43277655935056336No Hit
CCGGAA1042070.42923008832607595No Hit
TCAATG1038800.42788317075928467No Hit
AGAACG1036080.426762798960608No Hit
CACATT1025500.42240488218487326No Hit
TTCACA1021620.4208067047661728No Hit
ACCGCT1003180.41321124301338No Hit
GACCGA1002590.4129682211894023No Hit
GAGATA995410.4100107691620134No Hit
GAAGAT989670.4076464551456885No Hit
GTAAGA976470.4022093567109344No Hit
CATTAA975460.40179333630039643No Hit
CGTGAA964220.397163564602924No Hit
GCTGTC963310.3967887343320432No Hit
GCGCAG948070.39051135704828166No Hit
GGACTA945760.3895598648221997No Hit
ACGAAC927550.38205914039061845No Hit
AGTCAA924710.3808893404243532No Hit
CTGTTC910570.3750650546768211No Hit
CGTCAG904750.3726677885487705No Hit
CAGCCT898070.3699162872196676No Hit
CTATTA896750.3693725773761922No Hit
GTACGC892240.36751490207765125No Hit
ACGCTA882910.36367186204763186No Hit
AGGTCA874840.3603478177772935No Hit
AACCTT867110.35716381998178987No Hit
GCTTCT866770.35702377350695524No Hit
CGTTGC863640.355734522135684No Hit
GCACAT848710.34958483428485987No Hit
TCGTGG844100.3476859688466617No Hit
TGCATA838480.34537108299792546No Hit
GCTCAA828640.3413179732556542No Hit
AACATA823590.3392378712029642No Hit
GACTAA822780.33890423107174067No Hit
AGGACT822150.33864473319190014No Hit
AAGTTG821930.3385541148846542No Hit
GAGAAG820200.3378415254685842No Hit
CACCTA812340.33460398049152607No Hit
CTGGAG804670.3314446967798167No Hit
TGGCAT802160.33041082427442026No Hit
GACACG774810.31914532107816834No Hit
AGATGC765190.31518282964314304No Hit
CAAGCT754940.31096084032827714No Hit
CATACG752570.3099846340184009No Hit
CCATGA722260.29749990268829507No Hit
TTAGTA712600.2935209351974068No Hit
GGAGGC709900.29240880142666165No Hit
CGAATT709290.2921575415747526No Hit
CATGAG698530.28772548254763486No Hit
ATCCAC696510.28689344172655884No Hit
ACCAGG685180.2822265989033949No Hit
TAAGTC671830.276727715259155No Hit
AATGTA668740.27545493994374665No Hit
ACCTGC662730.27297941255034724No Hit
TCCTCC660510.2720649914499568No Hit
CTATGC658310.27115880837749773No Hit
TTCGTT657620.27087459641386286No Hit
AAGCAT655190.26987367602019224No Hit
TGTGAT650990.26814369015458867No Hit
TGCGGA646520.26630249091191055No Hit
AATTGC642990.2648484789820104No Hit
TTCTAC632240.2604205389688584No Hit
GCGTAT620640.25564248276861995No Hit
ATCCGG609660.2511198054342563No Hit
CCGCTC608230.250530786437158No Hit
AGGCTT593130.24431107534891652No Hit
TAATGA577920.23804605510705212No Hit
GGTACC570540.23500622280034872No Hit
TCAAGC554300.22831694412001488No Hit
CTCGTC554170.22826339693846048No Hit
CTGAGG514250.2118202931872951No Hit
AAGAGG494880.20384176313569002No Hit
GCGGCT484500.19956622663926973No Hit
CGCTTG482080.1985694252595648No Hit
GTGGTG477380.19663348869567507No Hit
GTTGGC464620.1913776268754128No Hit
CCACTT448200.18461420594369596No Hit
AGTGCA443380.18262884121221756No Hit
GAGCCA415090.17097615070318775No Hit
TCATGT405940.1672072529245514No Hit
ATTCGA391030.16106580310165872No Hit
GAACCG384930.1585532045825678No Hit
GGATCG376520.15508911383739493No Hit
CGAGAG375230.15455776103581667No Hit
CAGGAC361930.14907947246140535No Hit
TTCCAT353780.14572247607934127No Hit
TACGAA352780.14531057468276898No Hit
GATCCT351050.14459798526669895No Hit
ATTGCT340310.14017416426751267No Hit
CTTAGC320870.13216680111814752No Hit
GATTCA304110.12526333371159612No Hit
ACACGA304100.1252592146976304No Hit
TATTGG301430.12415943796878241No Hit
GGTAAT298470.12294020983492845No Hit
GCCTGG296180.12199695563677793No Hit
CCGTCG283450.11675345085841282No Hit
TCCAGA274410.11302986223339939No Hit
CGAAGC267230.11007241020601043No Hit
AGTTAG261710.10779871449693144No Hit
CGACAA248610.10240280620183458No Hit
GGCCGT246430.10150486115730699No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)