FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27204666
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAAC3656551.3440892823312No Hit
GAAGAT3105601.1415688764567078No Hit
CTACCG3028631.113275935826597No Hit
CTATTA2825331.0385461082301102No Hit
ACTTGA2516520.9250324925878524No Hit
AGACGG2343890.8615764663311801No Hit
AATCGG2233570.821024599236028No Hit
TGATAA2232750.8207231803544289No Hit
CTGTAT2188620.8045016983483642No Hit
AGTGCA2062090.7579912945815985No Hit
ATTCAT1949530.7166160393220781No Hit
GCTGTC1928770.7089849954415908No Hit
GCATAA1881200.6914990244688172No Hit
TCTCTG1869940.6873600286068574No Hit
AACTAG1846450.678725480401046No Hit
CTTAGC1837400.6753988451833961No Hit
AATTGC1836630.6751158055018944No Hit
AAGGCG1832870.6737336896545615No Hit
ATGCCG1829220.672392008047443No Hit
TGACCA1823870.6704254336370092No Hit
ACAGTG1818020.6682750672256002No Hit
TGTGAT1817480.668076571864547No Hit
GTCTCA1803480.6629303958372434No Hit
CCTGAC1803380.6628936374370484No Hit
ACTAGC1799690.6615372524698521No Hit
TTCTAC1774320.6522116463403741No Hit
GGTACC1770130.6506714693722025No Hit
ACTAAT1767370.6496569375268199No Hit
GAACCG1752130.6440549573370979No Hit
GATCAG1751850.6439520338165519No Hit
GCCAAT1749640.6431396731722419No Hit
CGATGT1746930.6421435205269567No Hit
TTGAAT1730970.6362768798558306No Hit
TGTGTG1729530.6357475588930223No Hit
GGTAAT1726500.6346337793671131No Hit
CGTACT1714190.6301088203031053No Hit
ACAGCC1698480.6243340756324669No Hit
GACTAA1696910.623756968749405No Hit
ACGAAC1669160.6135565126952854No Hit
AACATA1653030.6076273827438279No Hit
CTGTTC1647370.6055468572927893No Hit
TAGAGC1641780.6034920627218875No Hit
TTCCAT1638000.6021025951945156No Hit
GGCTAC1634500.6008160511876897No Hit
AGCCTA1624930.5972982722890258No Hit
CCTGCT1622970.5965778076452032No Hit
TACGAA1620660.5957286886006982No Hit
GTTACA1619050.5951368783575582No Hit
TTCTGT1616300.594126022352195No Hit
TACGGC1615080.5936775698698158No Hit
TCTTCA1601570.5887115100034678No Hit
AACCTT1584630.5824846370104305No Hit
GAGCAC1571140.5775259288241216No Hit
TCCAGA1556100.5719974654347897No Hit
GACACG1554970.571582095512586No Hit
CCTCGG1550930.570097056144707No Hit
TATTCT1546210.5683620596555018No Hit
TTACGT1533630.5637378529109676No Hit
GGATTC1514960.5568750595945563No Hit
TAGCTT1508370.5544526810217041No Hit
TTAGCG1506360.5537138371777841No Hit
TGCATA1504180.5529125040535326No Hit
TGACAC1502470.5522839354101977No Hit
ATGGCC1489420.5474869641847468No Hit
TCAATG1455920.5351729001194133No Hit
TGGCAT1454360.5345994690763709No Hit
CCTTAT1453680.5343495119550448No Hit
CTGAGG1441940.5300340757721488No Hit
GACCGA1426850.5244872331827194No Hit
CACATT1424250.5235315147776488No Hit
TACTCG1421080.5223662734914665No Hit
ACCGCT1417740.5211385429249527No Hit
CATGAG1404140.5161394004984292No Hit
GGCCGT1398260.5139780065669617No Hit
CATTAA1397750.513790538725967No Hit
GAGATA1390960.5112946433527249No Hit
TACAGT1390910.5112762641526274No Hit
TCCTCC1390030.5109527902309111No Hit
ATGTGA1389960.5109270593507746No Hit
CGTGAA1389700.5108314875102675No Hit
ACCAGG1371730.5042260029952215No Hit
AGTAGG1366220.5022006151444756No Hit
GTAAGA1360190.49998408361271557No Hit
CGCTTG1356470.4986166711254606No Hit
GAATAC1355120.4981204327228278No Hit
TGCGGA1343400.49381234821997083No Hit
AGGTGT1342710.4935587152586251No Hit
GTGGTG1330640.4891219763550856No Hit
CGTCAG1326740.4876883987474796No Hit
CATACG1326490.487596502746992No Hit
GGACTA1322570.486155573459347No Hit
AGTCAA1318450.484641127371312No Hit
GAGCCA1316680.48399050368786No Hit
CAAGGA1316010.48374422240655335No Hit
GCACAT1314330.4831266812832769No Hit
GTACGC1312170.48233269983906435No Hit
CAACGG1310350.48166369695551486No Hit
GTCTGC1304400.47947657214391093No Hit
ATTGAA1303860.4792780767828578No Hit
GCGTAT1303590.4791788291023312No Hit
TCGTGG1302810.47889211358080996No Hit
CAGCCT1300720.47812386301673393No Hit
TGCGAC1298860.47744015677310647No Hit
ACGCTA1296380.4765285484482699No Hit
CCGTCG1292610.4751427567609174No Hit
CCGCTC1275470.46884236696749004No Hit
GTACTT1275000.4686696024865734No Hit
CACCTA1273840.46824320504431116No Hit
TATGGT1273150.46798957208296543No Hit
CCACTT1266640.4655966002302693No Hit
AAGCGC1254780.46123705396713927No Hit
GAGTCG1254160.4610091518859301No Hit
TAAGTC1251370.4599835925204889No Hit
CTTCAC1243380.45704659634490646No Hit
AACTCT1239210.4555137710567738No Hit
AAGCAT1229510.4519482062378564No Hit
CGTCTC1228530.4515879739159452No Hit
AGAACG1226710.4509189710323957No Hit
GCTTCT1222390.4493310081439706No Hit
CGTTGC1219380.4482245802981003No Hit
TTCACA1217900.447680555975214No Hit
CTATGC1211210.44522141900216683No Hit
TTAGGC1208590.4442583489170571No Hit
CTCCGC1207370.4438098964346777No Hit
CCAGCG1206610.4435305325931955No Hit
AGATGC1202880.44215944426592113No Hit
CGAAGC1201370.4416043924229763No Hit
GGATCG1199090.4407663008985297No Hit
GGCCAG1197600.4402186007356238No Hit
CGAGAG1193340.43865269288731573No Hit
CAAGCT1192550.43836230152577504No Hit
CCGGAA1192170.43822261960503395No Hit
AGTTAG1186660.43619723175428804No Hit
AAGTTG1185320.43570466919167467No Hit
GAGAAG1180320.43386674918192336No Hit
ATACCT1170220.43015415076222585No Hit
AGGACT1155290.42466612161310857No Hit
AGTTCC1154740.424463950412036No Hit
TATAAG1153050.42384273344874No Hit
CGACAA1143760.42042787807062215No Hit
TCATGT1138530.4185054137404223No Hit
CCGACT1137670.41818929149874506No Hit
GATCCT1135600.4174283926147081No Hit
CAGGAC1129790.4152927295633771No Hit
CCATGA1118640.41119416794163177No Hit
ATTCGA1114190.40955841913295316No Hit
CTGGAG1112780.4090401256902033No Hit
GGCAGG1111910.4087203276085066No Hit
GACTTG1111900.40871665176848704No Hit
ATCCGG1110520.4082093858457957No Hit
CAGATC1100790.4046327935068197No Hit
TTAGTA1087380.39970349204066685No Hit
TAATGA1081420.3975126913890434No Hit
GCGCAG1079170.39668562738465524No Hit
GGCGTT1077190.39595781106079375No Hit
TCGGAC1059190.3893412990256892No Hit
GCCATA1056730.38843704238089155No Hit
AACAAT1042940.38336805899399756No Hit
GACCAT1036700.381074334821828No Hit
TCAAGC1035140.3805009037787856No Hit
ACCTGC1034690.380335490977908No Hit
AGGTCA1027120.3775528800831446No Hit
CTAGTT1020850.3752481283909165No Hit
ATCAGC1019110.37460853222752305No Hit
GTCGAG1016720.3737300064628619No Hit
GCGGCT1014170.3727926672578888No Hit
GGAGGC1008050.37054305316595326No Hit
CGAATT992990.36500723809658236No Hit
ATCCAC990500.3640919539317263No Hit
ACGTAA986460.36260691456384725No Hit
TATTGG983390.36147843167786No Hit
ATTGCT964800.35464504508160477No Hit
AATGTA961850.35356067227585153No Hit
ACACGA960230.35296518619269207No Hit
GGTGGT959820.3528144767518925No Hit
TTCGTT955420.35119710714331137No Hit
GCTCAA945580.3475800805641209No Hit
AGGCTT933160.3430146872598987No Hit
AGAGGT931670.34246698709699286No Hit
GTTGGC921260.3386404376366907No Hit
TCTCGC909190.33420369873315114No Hit
GATTCA869980.3197907300166817No Hit
GCCTGG848280.3118141571743612No Hit
GAGTGA820700.3016761904005732No Hit
CTCGTC817600.30053667999452743No Hit
CAGTCC816810.3002462886329867No Hit
CAGTAG803700.29542726236741884No Hit
AAGAGG768120.28234862357802887No Hit
TCCACG747790.27487564081838023No Hit
CGAGCC686720.2524272858192782No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)