FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22253507
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC2484771.1165745695723377No Hit
TTCCAT2468131.1090970964711315No Hit
GGCTAC2342411.0526026302281255No Hit
GACTAA2031430.9128583643018604No Hit
TGCGAC2003250.9001951917061882No Hit
CGTCAG2000870.8991256973563763No Hit
GAAGAT1947250.8750306187694371No Hit
GCTGTC1914200.8601790270630153No Hit
TCTTCA1911990.8591859251667614No Hit
CTATTA1849130.8309386920452583No Hit
AGTCAA1746040.7846134094729429No Hit
CTACCG1734150.779270431397622No Hit
TTCACA1733810.779117646490506No Hit
AATCGG1728220.7766056828705695No Hit
AGTGCA1712800.7696764379654856No Hit
TACGAA1711620.7691461844643184No Hit
CATAAC1701430.7645671309245774No Hit
ACAGTG1695520.7619113697450024No Hit
ATCCAC1674450.7524431991775499No Hit
ACTTGA1657590.7448668652540924No Hit
ACCAGG1655050.743725472124461No Hit
GTACGC1644760.7391014818473332No Hit
TATTCT1639020.7365221131213161No Hit
AGTTCC1613270.7249509032441493No Hit
TAGCTT1568010.7046125359027681No Hit
GCCAAT1532880.6888262600586954No Hit
AGATGC1524190.6849212575797603No Hit
AACATA1521140.6835506870894552No Hit
TTGAAT1499620.6738803012037609No Hit
TGACCA1489320.6692518172528942No Hit
ACCGCT1478800.6645244724797759No Hit
CAAGCT1475960.6632482691379835No Hit
GGATTC1466640.65906016521351No Hit
CGATGT1456650.6545709851485431No Hit
ACTAAT1445410.649520095866238No Hit
AGAACG1424690.6402092038796402No Hit
GTAAGA1402700.6303276153282267No Hit
TGCGGA1399350.6288222346257604No Hit
GTTGGC1388400.6239016618818778No Hit
TAAGTC1386000.6228231801845885No Hit
CAGATC1381280.6207021661799195No Hit
GAACCG1369610.6154580489268501No Hit
CTTAGC1349390.6063718406271875No Hit
TATGGT1333080.5990426587593587No Hit
GATCAG1328270.5968812016910413No Hit
GCTCAA1327840.5966879737202769No Hit
GCACAT1326740.5961936696090193No Hit
GGAGGC1322230.5941670227528631No Hit
AGGCTT1321410.5937985415062894No Hit
ATGTGA1319700.5930301232969707No Hit
TCGTGG1318830.5926391736817033No Hit
AAGCAT1314330.5906170204992858No Hit
TCGGAC1311840.5894980957383481No Hit
ACCTGC1310840.5890487283644776No Hit
TACTCG1305500.5866491065880088No Hit
GTCTCA1289510.5794637222798186No Hit
CGAAGC1283660.5768349231426759No Hit
TCCAGA1280000.5751902385543096No Hit
CACCTA1279500.5749655548673743No Hit
AAGTTG1279290.5748711877188616No Hit
GACCGA1248540.561053140972342No Hit
TTAGTA1240960.5576469362784032No Hit
GACACG1239470.5569773788913361No Hit
GCATAA1236840.5557955426980565No Hit
TCTCTG1226980.5513647803916929No Hit
GACCAT1214850.5459139541466431No Hit
AGGTGT1214300.5456668020910143No Hit
TAATGA1211340.5443366746643574No Hit
CGTGAA1209960.543716547688416No Hit
CGAATT1205420.5416764198110438No Hit
CAGCCT1204900.541442748776631No Hit
ATTCGA1204550.5412854701957763No Hit
CATGAG1203490.5408091407794736No Hit
TGGCAT1187400.5335788197338963No Hit
CAGGAC1186950.5333766044156546No Hit
TTAGGC1169980.5257508400810713No Hit
GGCGTT1163000.5226142558114548No Hit
ATACCT1156260.5195855197115673No Hit
TCCACG1155460.5192260258124708No Hit
ACACGA1140000.512278806212432No Hit
CCTGCT1140000.512278806212432No Hit
CGTACT1136690.5107914002049205No Hit
GAGATA1135760.5103734885472209No Hit
CGAGCC1115460.5012513308576486No Hit
GTCTGC1112270.4998178489350016No Hit
GTTACA1111140.49931006380252785No Hit
ATTGCT1105910.49695987243718487No Hit
TCAAGC1100410.4944883518808968No Hit
CTATGC1099630.4941378453292778No Hit
GAGAAG1097620.49323461690779796No Hit
GGCCAG1094990.4920527807145184No Hit
CCTGAC1092230.4908125267626357No Hit
CGAGAG1090170.48988682997246236No Hit
CTCCGC1087110.4885117658084184No Hit
GGACTA1084530.48735239798383234No Hit
GCGCAG1082940.4866379038593782No Hit
CCATGA1081070.4857975868702403No Hit
AGTAGG1080620.4855953715519985No Hit
TGTGTG1079220.4849662572285797No Hit
GCCATA1070500.48104777372842855No Hit
GATCCT1070420.4810118243385188No Hit
CCTTAT1065870.4789672027874078No Hit
CAGTCC1063910.4780864427346216No Hit
CATTAA1059570.4761361883320233No Hit
AATGTA1056500.4747566304942407No Hit
TTACGT1055850.4744645417012249No Hit
ACGCTA1051140.4723480213702945No Hit
TATTGG1040090.46738251188902497No Hit
TGTGAT1037120.4660478907886294No Hit
CTCGTC1028960.46238105301784566No Hit
AACAAT1025020.4606105455647957No Hit
AGTTAG1024360.4603139630980411No Hit
AGAGGT1017020.4570156065738313No Hit
AGCCTA1013090.45524959279452No Hit
GAGCCA1009960.4538430729143052No Hit
TCCTCC1009150.45347908534147No Hit
TTCTGT1002110.4503155390294213No Hit
TATAAG1000720.44969091837974123No Hit
AGACGG999640.44920560161596107No Hit
CCGACT999170.4489943989502419No Hit
CACATT995680.44742610681543366No Hit
CTAGTT994210.4467655367758439No Hit
GCCTGG988030.4439884464053239No Hit
TTCTAC986030.44308971165758276No Hit
ACTAGC985090.4426673063261445No Hit
CTTCAC984550.44242464794425435No Hit
CCACTT977180.4391128103988283No Hit
ACAGCC968680.4352931877209287No Hit
CGACAA951040.4273663472458521No Hit
CCGGAA949650.42674172659617204No Hit
AGGACT948470.4262114730950048No Hit
TGCATA947440.4257486246999181No Hit
GCGGCT944730.42453083911672895No Hit
GAGTGA937680.4213627991309415No Hit
CCGCTC929300.4175971005379062No Hit
GATTCA928180.41709380907917115No Hit
AATTGC925310.41580412471616274No Hit
GGATCG921190.41395273113581604No Hit
AAGGCG909640.4087625379676111No Hit
ATGGCC904860.4066145619205099No Hit
AACTAG897700.4033970915235967No Hit
GACTTG895440.4023815212586493No Hit
ACGTAA891850.400768292386454No Hit
TTAGCG887380.3987596202252526No Hit
GCGTAT886070.39817094896548216No Hit
GGTGGT883450.3969936064459413No Hit
TGACAC879130.39505233939082046No Hit
ATCAGC876500.3938705031975409No Hit
CTGGAG876450.3938480348288474No Hit
AACCTT872260.3919651855323298No Hit
GAGTCG867150.38966891825185124No Hit
CAACGG847810.3809781532411947No Hit
ATTCAT843640.3791042912921545No Hit
TTCGTT836320.3758149221154221No Hit
GAGCAC832720.3741971995694881No Hit
AGGTCA828520.37230985659923177No Hit
TCAATG807420.36282820501056307No Hit
CGTTGC807370.36280573664186955No Hit
TCATGT805120.3617946600506608No Hit
TAGAGC804120.3613452926767902No Hit
TGATAA803820.36121048246462906No Hit
GCTTCT798690.3589052278366731No Hit
TCTCGC789710.3548699088193155No Hit
CGCTTG787900.3540565538726098No Hit
GTACTT775950.3486866137548567No Hit
AAGAGG766960.34464680106376044No Hit
CCGTCG761560.3422202172448594No Hit
CAGTAG753860.33876008846605615No Hit
GAATAC745140.3348416049659049No Hit
AACTCT742780.3337810979635704No Hit
ATTGAA733290.32951660158553886No Hit
GGCAGG729150.32765622065771477No Hit
CTGTAT726250.3263530552734901No Hit
CAAGGA718140.3227086858714No Hit
GTGGTG715250.321410014160914No Hit
TACGGC700670.3148582378498814No Hit
GGTAAT699910.31451671864573977No Hit
CCTCGG694760.31220247667030643No Hit
CTGTTC688780.30951525977456046No Hit
ACGAAC684560.30761892945682673No Hit
GGCCGT682350.3066258275605728No Hit
CTGAGG664540.29862259463193824No Hit
TACAGT662080.29751715089221664No Hit
CCAGCG636800.28615714368076905No Hit
ATCCGG631380.2837215725143906No Hit
ATGCCG622380.2796772661495557No Hit
CGTCTC603230.2710718809399345No Hit
CATACG570810.25650339067905115No Hit
AAGCGC524760.2358100231123121No Hit
GTCGAG412420.18532809233169406No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)