FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29237724
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3174271.0856761627546658No Hit
TTCCAT3123781.068407376716464No Hit
GGCTAC3012911.0304871884008482No Hit
TGCGAC2598550.8887661707183501No Hit
GACTAA2587700.8850552115479302No Hit
CGTCAG2538510.8682310565624055No Hit
GCTGTC2454500.8394976298428702No Hit
TCTTCA2427190.8301569574977861No Hit
GAAGAT2413000.8253036385458731No Hit
CTATTA2372430.8114277294634835No Hit
AGTCAA2209890.7558351669233898No Hit
CTACCG2204370.7539471950689458No Hit
CATAAC2203680.7537111985871403No Hit
TACGAA2203560.7536701557207394No Hit
AATCGG2193730.7503080609147278No Hit
TTCACA2184390.7471135578131868No Hit
AGTGCA2164410.7402799205574278No Hit
ATCCAC2148670.7348964645811692No Hit
ACAGTG2143150.7330084927267252No Hit
ACCAGG2132230.7292735918842383No Hit
ACTTGA2123850.726407431713905No Hit
GTACGC2121440.725583154147019No Hit
TATTCT2063390.7057286675255571No Hit
AGTTCC2018130.6902486664146634No Hit
TAGCTT1975330.6756100440649895No Hit
AGATGC1963470.6715536407690283No Hit
GCCAAT1953270.6680649971249472No Hit
AACATA1926100.6587722081239976No Hit
ACCGCT1908430.652728646046457No Hit
TGACCA1903960.6511997992730214No Hit
CGATGT1889860.6463772624709091No Hit
CAAGCT1879540.6428475759604271No Hit
TTGAAT1865860.6381686891907181No Hit
ACTAAT1834280.6273675748495334No Hit
TGCGGA1816860.6214095187436615No Hit
GGATTC1791170.6126229250949903No Hit
AGAACG1783390.6099619792566617No Hit
GTTGGC1769470.6052010067541509No Hit
CTTAGC1747190.5975807145590403No Hit
GTAAGA1747040.5975294109760391No Hit
TAAGTC1741920.5957782486762649No Hit
CAGATC1737410.5942357209473624No Hit
GAACCG1732710.5926282086799917No Hit
GGAGGC1717150.5873063170033345No Hit
GCTCAA1702460.5822819861080842No Hit
ACCTGC1702360.5822477837194168No Hit
TCGTGG1696080.5800998737111001No Hit
GCACAT1688650.5775586362331075No Hit
AGGCTT1680700.5748395463340443No Hit
TCGGAC1680550.5747882427510431No Hit
CGAAGC1674990.5728865899411322No Hit
ATGTGA1673410.5723461922001862No Hit
GATCAG1662470.5686044508799659No Hit
TATGGT1655100.5660837348351739No Hit
TACTCG1653340.5654817727946265No Hit
AAGCAT1652590.5652252548796205No Hit
TCCAGA1650930.5646574952277407No Hit
GTCTCA1636630.5597665536482935No Hit
CACCTA1629460.5573142423808365No Hit
GCATAA1584200.5418342412699428No Hit
GACCGA1580460.5405550719337798No Hit
AAGTTG1570530.5371587747391007No Hit
TGGCAT1562160.5342960348076341No Hit
TCTCTG1557910.532842433289267No Hit
GACACG1544200.5281532858029578No Hit
CAGCCT1539650.5265970771185884No Hit
CGTGAA1537550.5258788269565715No Hit
GACCAT1536730.5255983673694984No Hit
CAGGAC1534000.5246646421588766No Hit
CATGAG1530220.5233717918672466No Hit
CGAATT1524490.521411994996601No Hit
TAATGA1520390.520009697061235No Hit
ATTCGA1515860.5184603288545989No Hit
AGGTGT1502410.5138601075788252No Hit
TCCACG1480090.5062261344282476No Hit
TTAGTA1474160.504197932780267No Hit
ACACGA1473770.5040645434644639No Hit
ATACCT1470120.5028161562781015No Hit
TTAGGC1466390.5015404071808052No Hit
CCTGCT1464060.5007434915248533No Hit
GGCGTT1456710.4982296159577948No Hit
CGTACT1453650.4971830228645705No Hit
CGAGCC1446220.494641785386578No Hit
GTCTGC1429940.48907363651151503No Hit
CCTGAC1424580.487240388478939No Hit
CTATGC1414410.4837620055514581No Hit
GAGATA1414010.48362519599678827No Hit
CCATGA1394780.4770480766560352No Hit
CTCCGC1394240.47686338375723086No Hit
CGAGAG1392990.4764358538988876No Hit
TCAAGC1385520.4738809354654282No Hit
GGCCAG1381090.4723657696474596No Hit
GTTACA1374870.4702383810723434No Hit
GCCATA1369060.4682512222907638No Hit
ATTGCT1366380.46733459827447577No Hit
CAGTCC1354250.4631858485291126No Hit
GCGCAG1352770.4626796531768342No Hit
GGACTA1352470.4625770460108317No Hit
GATCCT1351270.46216661734682224No Hit
GAGAAG1350910.46204348874761936No Hit
ACGCTA1348030.46105845995399647No Hit
TGTGTG1345710.46026496453691124No Hit
TTACGT1343890.45964248106316347No Hit
CCTTAT1343140.4593859631481575No Hit
CATTAA1340150.4583633117270004No Hit
AGTAGG1328060.4542282429371041No Hit
TATTGG1318080.45081484454809134No Hit
CTCGTC1315390.4498948002929366No Hit
AATGTA1309200.44777767243442074No Hit
TCCTCC1308950.44769216646275206No Hit
AGACGG1307230.4471038853776717No Hit
AACAAT1297530.4437862536769278No Hit
TGTGAT1293810.4425139248184982No Hit
GAGCCA1281220.43820784408526464No Hit
AGCCTA1278380.43723649624710875No Hit
CCGACT1277230.43684316877743284No Hit
CACATT1274220.4358136768785423No Hit
CTTCAC1270010.4343737563156421No Hit
GCGGCT1270000.43437033607677533No Hit
ACTAGC1268480.4338504597690299No Hit
AGTTAG1267400.4334810739714213No Hit
AGAGGT1265250.4327457226150708No Hit
TTCTGT1259270.4307004197727566No Hit
CTAGTT1259000.43060807332335443No Hit
GCCTGG1254690.42913395037178675No Hit
TATAAG1252280.4283096728049009No Hit
TTCTAC1249860.42748197499914836No Hit
CCACTT1248030.4268560712865338No Hit
ACAGCC1247090.4265345688330597No Hit
CCGGAA1232870.4216709891645465No Hit
CGACAA1221240.4176932513625205No Hit
TGCATA1214410.41535722821653287No Hit
CCGCTC1210540.414033595775102No Hit
AATTGC1179920.40356082436512497No Hit
ATGGCC1170130.40021241051458045No Hit
GAGTGA1167450.3992957864982924No Hit
GATTCA1161310.3971957598341102No Hit
AGGACT1159950.39673060734823273No Hit
AAGGCG1155100.3950717914978608No Hit
GGATCG1148810.3929204612506774No Hit
ACGTAA1136480.3887033067279792No Hit
TGACAC1129280.38624073474392195No Hit
AACTAG1127090.3854917024321045No Hit
ATCAGC1126050.38513599758996286No Hit
GCGTAT1124520.3846127010433507No Hit
GACTTG1120190.38313173761404956No Hit
AACCTT1105700.3781758114961343No Hit
CTGGAG1098330.37565509545134224No Hit
GGTGGT1090230.37288470196927775No Hit
TTAGCG1088540.37230668160079766No Hit
GAGTCG1079230.3691224392158569No Hit
CAACGG1074420.36747730432095194No Hit
GAGCAC1052190.359874113320175No Hit
ATTCAT1045000.3574149615749844No Hit
TTCGTT1043720.3569771710000409No Hit
AGGTCA1029680.3521751556311291No Hit
CGTTGC1028100.3516347578901832No Hit
TAGAGC1025630.35078995889009695No Hit
TCATGT1023170.3499485801288773No Hit
TGATAA1021110.3492440109223276No Hit
TCTCGC1017520.3480161451691657No Hit
TCAATG1015090.34718502712454635No Hit
GCTTCT999600.3418870771199564No Hit
CGCTTG999430.3418289330592217No Hit
CCGTCG987570.3377725297632606No Hit
GTACTT970560.33195470345092526No Hit
AAGAGG958590.32786067752743No Hit
GAATAC955250.32671831774593674No Hit
CAGTAG954510.3264652200697975No Hit
AACTCT953960.32627710693212647No Hit
TACGGC944160.3229252728427151No Hit
GGCAGG931660.3186499742592823No Hit
CAAGGA920560.31485350911719395No Hit
ATTGAA911230.3116624262545197No Hit
CTGTAT902260.3085944719910483No Hit
CCTCGG896920.3067680644362058No Hit
ACGAAC889500.30423024719708003No Hit
GTGGTG883960.3023354348649026No Hit
CTGTTC875430.2994179711115681No Hit
GGCCGT872260.2983337553908095No Hit
GGTAAT856450.29292635774248366No Hit
TACAGT842620.28819616738977355No Hit
CTGAGG841840.28792938875816737No Hit
CATACG841040.28765576964882766No Hit
CCAGCG813710.2783082568260101No Hit
ATCCGG804080.2750145667973335No Hit
CGTCTC775320.2651779598165712No Hit
ATGCCG768250.2627598509377816No Hit
AAGCGC698390.23886606221469225No Hit
GTCGAG522110.1785740914716891No Hit
GNNNNN311670.10659858475988075No Hit
ANNNNN307720.105247590407516No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)