FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002208504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21320339
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC7542253.537584463361488No Hit
GAAGAT5938812.785513870112478No Hit
CTATTA5676522.6624904979231334No Hit
GGCTAC4603172.1590510357269648No Hit
CAGGAC3682181.727073851874494No Hit
TCCACG3608541.6925340633655028No Hit
ACACGA3548421.6643356374399114No Hit
CGAGCC3467061.626174893372943No Hit
CGAGAG3348381.57050973720446No Hit
GATCCT3312721.553783924355049No Hit
ACTTGA3269701.5336060087975147No Hit
ACAGTG3255611.5269972958685132No Hit
TATTGG3210981.5060642328435772No Hit
GAGCCA3097081.4526410672925978No Hit
TAGCTT3063641.4369565136839522No Hit
GCCAAT2968391.39228086382679No Hit
TGACCA2895001.3578583342413082No Hit
CGATGT2851461.3374365201228744No Hit
GGATCG2839941.3320332289275512No Hit
CAGATC2671251.2529115977002054No Hit
GATCAG2594301.2168193010439468No Hit
TTAGGC2381331.1169287692845784No Hit
CGTCAG2106180.9878735980698994No Hit
TGCGAC1810110.8490062001359359No Hit
GTACGC1756870.8240347397853289No Hit
TCTTCA1707290.8007799500749027No Hit
TTGAAT1585500.74365609289796No Hit
TTCCAT1584460.7431682957761601No Hit
CTACCG1546440.7253355586888183No Hit
TTCACA1527890.716634946564405No Hit
GGAGGC1478240.693347324355396No Hit
GACTAA1395500.6545393110306548No Hit
AGTCAA1368420.6418378244360936No Hit
AATCGG1353450.634816360096338No Hit
GCTGTC1344590.6306607038471573No Hit
ATCCAC1311780.615271642725756No Hit
TCTCTG1306530.6128092053320541No Hit
CGTGAA1301940.6106563314964176No Hit
TATTCT1286430.6033815878818812No Hit
CAGCCT1280540.6006189676439948No Hit
AGAACG1271160.5962194128339141No Hit
CATAAC1215370.5700519114635091No Hit
GCTCAA1201290.5634478888914478No Hit
CTTAGC1192620.5593813494241343No Hit
ATGTGA1189670.557997694126721No Hit
ACCGCT1166090.5469378324612943No Hit
ACCAGG1161760.5449069079061079No Hit
GAGAAG1161710.5448834561214059No Hit
GGATTC1158020.543152714410404No Hit
ACGCTA1132550.5312063752832448No Hit
TTACGT1124600.5274775415156392No Hit
TACGAA1113230.522144605674422No Hit
TCCAGA1111100.5211455596461201No Hit
AGTGCA1111080.5211361789322393No Hit
AGATGC1094180.5132094757029896No Hit
GTAAGA1092920.5126184907285011No Hit
CTCGTC1088380.5104890686775665No Hit
AACATA1055300.49497336791877466No Hit
ACTAAT1050910.49291430122194585No Hit
CAAGCT1045500.49037681811719785No Hit
AGTTCC1042770.48909635067247287No Hit
CGTACT1023620.48011431713163666No Hit
TACTCG1023550.4800814846330539No Hit
GTTGGC996240.46727211982886385No Hit
AAGGCG981680.46044296012366404No Hit
TGCGGA980900.46007711228231407No Hit
GCGCAG970310.45511002428244696No Hit
TCGTGG952980.44698163570476057No Hit
AGGTGT948450.4448569040107664No Hit
CGAATT947590.44445353331389337No Hit
GCACAT945680.44355767513827987No Hit
ACCTGC941490.44159241558025886No Hit
AGGCTT940490.44112337988622036No Hit
TAAGTC929760.43609062688918787No Hit
CACCTA912410.4279528575976207No Hit
GAGATA897260.42084696683293826No Hit
GAACCG886410.4157579295526211No Hit
AAGCAT877750.4116960804422481No Hit
GTCTCA876890.4112927097453751No Hit
CCGGAA873420.40966515588706165No Hit
TTAGTA870900.40848318593808475No Hit
GACACG851470.3993698224029177No Hit
TCGGAC850730.3990227359893292No Hit
CGAAGC849540.39846458351342345No Hit
GGACTA848760.39809873567207354No Hit
TGGCAT840420.3941869779837928No Hit
CTCCGC839420.39371794228975443No Hit
CATGAG837950.3930284598195179No Hit
CCTGCT809200.37954368361591245No Hit
GTCTGC807740.37885889150261637No Hit
GACCAT804410.3772970026414683No Hit
TAATGA802230.3762745048284645No Hit
AGAGGT791630.37130272647165696No Hit
CACATT783870.36766300948591857No Hit
CCATGA774100.36308053075516294No Hit
ATACCT756340.3547504568290401No Hit
CTATGC742880.3484372363872826No Hit
AGGTCA742350.3481886474694422No Hit
ATTCGA724330.3397366242628694No Hit
TCAAGC723850.3395114871297309No Hit
TGCATA717770.3366597501099771No Hit
TCCTCC711700.33381270344716374No Hit
CCTGAC702150.32933341256909654No Hit
GCCATA694980.32597042664284087No Hit
CAGTCC690800.3240098574417602No Hit
CTGGAG689400.3233532074701064No Hit
GCGGCT680130.3190052465863699No Hit
TATAAG669410.31397718394627777No Hit
TGTGAT664600.31172112225795284No Hit
AGTTAG661470.3102530405356125No Hit
CCGCTC658330.3087802684563318No Hit
TTCTGT653620.3065711103374107No Hit
AACCTT649560.30466682541961454No Hit
TTCTAC646090.30303927156130117No Hit
CTAGTT642350.30128507806559734No Hit
ATTGCT637050.2987991888871936No Hit
CCACTT636040.29832546283621475No Hit
CGACAA622820.29212481096102644No Hit
CTTCAC621740.29161825241146494No Hit
GCGTAT620030.2908162013746592No Hit
TCTCGC614340.2881473882755804No Hit
ATTCAT599700.2812807057148575No Hit
GGCCGT594120.278663486542123No Hit
GGTAAT593030.2781522376356211No Hit
GCCTGG588010.27579767845154807No Hit
GACTTG585740.2747329674260808No Hit
ATCAGC574420.26942348336956556No Hit
TGATAA573750.2691092294545598No Hit
CCAGCG572300.26842912769820404No Hit
TTAGCG562310.26374346111476No Hit
GATTCA551680.25875761168713124No Hit
TAGAGC551150.2585090227692909No Hit
AAGAGG547680.25688146891097746No Hit
TGACAC541100.25379521404420446No Hit
AATTGC530310.2487343189055296No Hit
GTACTT528080.24768836930782384No Hit
ATTGAA526920.24714428790273923No Hit
ACGAAC520470.24411900767619127No Hit
CTGTTC519060.24345766734759708No Hit
TCATGT517030.24250552488869903No Hit
CTGTAT508390.2384530564922068No Hit
AACTCT505550.2371209951211376No Hit
CCGTCG503330.23607973588037223No Hit
AAGTTG499160.23412385703623192No Hit
CAAGGA483730.2268866362772187No Hit
GACCGA480330.22529191491748793No Hit
CATACG472090.22142706079861116No Hit
ATCCGG471470.2211362586683073No Hit
CGTCTC469590.220254471563515No Hit
AAGCGC464870.21804062308765354No Hit
GTGGTG461960.21667572921800163No Hit
CGCTTG459510.21552659176760744No Hit
GGCAGG453150.2125435247535229No Hit
ACTAGC448510.21036719913318452No Hit
TACAGT442710.2076467921077615No Hit
AGGACT430090.2017275616489963No Hit
CTGAGG425880.1997529213770944No Hit
TGTGTG418600.19633834152449453No Hit
GGCCAG410850.19270331489569656No Hit
AATGTA402820.1889369582725678No Hit
CCTTAT395000.18526909914518713No Hit
CATTAA389270.18258152461834684No Hit
GAGCAC378140.1773611573436989No Hit
GGCGTT369110.1731257650265317No Hit
CGTTGC367690.1724597343409971No Hit
TCAATG363120.17031624121924138No Hit
GCTTCT362010.16979561159885873No Hit
TATGGT360820.16923745912295296No Hit
GTCGAG351010.16463621896443578No Hit
ATGGCC348380.16340265508911467No Hit
GCATAA341900.16036330379174554No Hit
AGACGG331380.15542904829046106No Hit
AACAAT329300.1544534540468611No Hit
CCGACT316500.14844979716316895No Hit
TTCGTT310260.14552301443236904No Hit
AGCCTA306870.1439329834295787No Hit
GTTACA305110.143107480608071No Hit
ACAGCC304520.14283074954858832No Hit
AGTAGG291720.13682709266489618No Hit
GAATAC276570.1297212019002137No Hit
AACTAG276280.12958518154894252No Hit
GGTGGT274080.12855330302205795No Hit
CAGTAG272720.12791541447816565No Hit
CAACGG267170.12531226637625228No Hit
GAGTCG266260.12488544389467728No Hit
GAGTGA249290.11692590816684482No Hit
ACGTAA238620.11192129731145456No Hit
TACGGC235250.11034064702254501No Hit
ATGCCG228140.10700580323793163No Hit
CCTCGG217790.10215128380463367No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)