FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21842170
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAA4070781.8637250785979598No Hit
GGAGGC3166701.4498101608036198No Hit
TTGAAT2940941.3464504671468083No Hit
GGCTAC2915881.3349772481397224No Hit
GTACGC2901271.328288352301992No Hit
ACCGCT2898411.3269789585924843No Hit
CGTCAG2786701.2758347728270587No Hit
AACATA2604031.1922029725068526No Hit
ACGCTA2498411.1438469712487358No Hit
CAGCCT2362581.0816599266464826No Hit
TCTCTG2330371.066913223365627No Hit
TTACGT2108030.9651193082006047No Hit
TCCTCC2021970.9257184611235971No Hit
GAGAAG1991110.911589828300027No Hit
TATTCT1977210.9052259917398318No Hit
TACTCG1915530.8769870392914257No Hit
ATTGCT1906670.8729306657717617No Hit
TGCGGA1898430.8691581468324805No Hit
ATACCT1891630.8660449030476367No Hit
GACCAT1873840.8579001079105235No Hit
ATTCGA1834500.8398890769552658No Hit
GCGTAT1787760.8184901042341489No Hit
GCGGCT1786770.8180368525654732No Hit
CTCCGC1783030.8163245684838091No Hit
TCTCGC1779080.8145161401087895No Hit
CTTCAC1729070.7916200633911374No Hit
ACCAGG1723390.7890195891708562No Hit
ATTCAT1688620.7731008411710009No Hit
AGGTGT1653980.7572416110670322No Hit
CCGCTC1629510.7460385117412784No Hit
CATGAG1609810.7370192613645988No Hit
GCTGTC1607990.7361860108221848No Hit
CTCGTC1578350.722615930560013No Hit
AGTCAA1575350.7212424406549349No Hit
GCCAAT1553190.7110969285560913No Hit
AGATGC1547650.7085605505313803No Hit
GGATTC1537820.7040600819424078No Hit
GTAAGA1531210.7010338258515523No Hit
TGACCA1520880.6963044422784No Hit
TTCTGT1512630.6925273450394352No Hit
GATTCA1510990.6917765038913258No Hit
TTCCAT1506440.6896933775352907No Hit
AATCGG1489880.6821117132592595No Hit
TGATAA1456750.6669438064075136No Hit
GACACG1451270.6644348981809042No Hit
ATCCAC1445730.6618985201561933No Hit
TCCAGA1433440.6562717898450565No Hit
GATCAG1430280.654825047145041No Hit
CGATGT1405760.6435990563208692No Hit
TGACAC1404030.6428070104756075No Hit
AATTGC1400540.6412091838860333No Hit
AGCCTA1350810.6184412995595218No Hit
CGAATT1334370.6109145748796938No Hit
TCGGAC1331200.6094632538799946No Hit
ACCTGC1320300.6044729072248775No Hit
CTAGTT1264770.5790496090818815No Hit
CGTGAA1257960.5759317869973541No Hit
AGTGCA1238910.5672101261001081No Hit
CGTCTC1234230.5650674818481863No Hit
CATAAC1232610.5643257972994442No Hit
CACCTA1218490.5578612381462098No Hit
GCACAT1216940.5571516016952528No Hit
ACTTGA1194410.5468366925081162No Hit
TGTGAT1194220.5467497048141279No Hit
GCCTGG1192660.5460354900634873No Hit
GTCTCA1190810.5451885046220225No Hit
CAAGCT1177060.5388933425570811No Hit
CTATTA1164600.5331887811513234No Hit
TCGTGG1136150.5201635185514992No Hit
AAGGCG1130340.5175035264353314No Hit
TGTGTG1126530.515759194255882No Hit
ACAGTG1122670.5139919705780149No Hit
AAGCGC1122110.5137355857957336No Hit
GTACTT1120780.5131266719378157No Hit
GGTGGT1101410.5042585054506947No Hit
CGAGAG1100170.5036907962899291No Hit
TCTTCA1093170.5004859865114134No Hit
GACCGA1087720.49799081318385485No Hit
GGCGTT1072760.49114167685719873No Hit
CATTAA1068450.4891684296935699No Hit
TTAGTA1043130.47757617489471055No Hit
GGTAAT1019480.46674849614301145No Hit
CCACTT1018400.4662540397771833No Hit
TTCACA1009640.4622434492543552No Hit
TGCGAC1002490.45896996498058573No Hit
AGACGG990520.45348974025932404No Hit
AGAGGT987650.45217576825013267No Hit
TTCGTT981970.4495752940298514No Hit
TTCTAC981310.44927312625073423No Hit
GGCCGT979060.44824300882192564No Hit
TTAGGC973660.4457707269927851No Hit
AGAACG968320.443325914961746No Hit
CGCTTG966050.4422866409335703No Hit
TAGAGC960750.43986014210126556No Hit
CTTAGC957980.43859195308891014No Hit
GAGCAC950530.4351811198246328No Hit
AGGCTT948060.43405027980278515No Hit
AAGCAT945040.43266763329833985No Hit
TAAGTC939180.429984749683754No Hit
CCGACT932160.42677078330587115No Hit
AAGTTG927360.4245731994577462No Hit
CTGAGG924160.4231081435589962No Hit
CAGATC920880.42160646126277745No Hit
TACAGT917250.4199445384776329No Hit
GAACCG911540.41733032935830094No Hit
ATCAGC910900.41703731817855094No Hit
TATGGT906530.41503660121682046No Hit
ATGTGA900650.4123445610028674No Hit
GTTGGC898820.4115067321607697No Hit
ACTAGC894570.4095609547952424No Hit
CCATGA890600.4077433698208557No Hit
CCTGCT882240.4039159112853714No Hit
TCATGT879280.40256073457902763No Hit
AGGTCA877950.4019518207211097No Hit
GTTACA875650.40089881179388315No Hit
CGTACT870670.3986188185514534No Hit
GCTCAA867600.3972132805485902No Hit
AACAAT848590.38850993285007857No Hit
TATTGG834900.38224224058323875No Hit
GGATCG830090.3800400784354302No Hit
ACTAAT825440.3779111690825591No Hit
GTGGTG823910.3772106892309693No Hit
TTAGCG806150.3690796289929068No Hit
CACATT804560.36835167934321544No Hit
GGACTA803810.3680083068669459No Hit
GTCGAG802320.36732614021409044No Hit
CCTGAC800900.36667602165902013No Hit
TCAATG799290.3659389154099616No Hit
GATCCT796660.3647348225931764No Hit
GGCAGG796110.36448301611057876No Hit
GAGATA788840.3611545922406061No Hit
GCATAA785150.35946519965736007No Hit
AAGAGG775990.3552714771471882No Hit
CGACAA771310.35312883289526636No Hit
CTGGAG764060.3498095656246609No Hit
AGGACT762520.3491045074733875No Hit
GGTACC759400.34767607797210626No Hit
TAATGA758560.34729150079868437No Hit
ACGTAA735580.33677056812578604No Hit
GAGCCA724500.33169781207636423No Hit
TATAAG721900.3305074541586298No Hit
AACTAG716140.32787035354087984No Hit
CCGTCG712840.32635951464529395No Hit
TAGCTT708300.3242809665889424No Hit
AACCTT702840.32178121496170026No Hit
CCTTAT700250.3205954353436494No Hit
CTGTAT700000.3204809778515596No Hit
AGTAGG694790.3180956837164073No Hit
GTCTGC690120.31595761776416903No Hit
CAAGGA688820.31536243880530185No Hit
CAGTCC682070.3122720865188761No Hit
AGTTCC680740.31166317266095817No Hit
TCAAGC676330.3096441425004933No Hit
CTACCG657940.3012246493823645No Hit
TACGAA653210.29905911363202464No Hit
GCTTCT638210.29219166410663405No Hit
AATGTA637220.29173841243795834No Hit
CAGTAG632020.2893576966024896No Hit
AGTTAG627260.28717842595309895No Hit
GAGTGA624370.2858552973445404No Hit
GCGCAG622270.2848938544109857No Hit
AACTCT616830.2824032593831107No Hit
GAGTCG611330.2798851945571342No Hit
CCAGCG601130.2752153288798686No Hit
ACACGA595430.2726056980602202No Hit
ATCCGG589870.2700601634361421No Hit
GAATAC584360.26753752031048195No Hit
ATGCCG535440.24514047825834157No Hit
ATTGAA523470.23966025353707987No Hit
GAAGAT518860.23754965738294317No Hit
TGGCAT505900.2316161809930057No Hit
GGCCAG491650.2250921039438847No Hit
TACGGC484330.2217407885754941No Hit
CAACGG479330.21945163873369725No Hit
CTATGC457430.20942516242662704No Hit
CGTTGC451140.20654541192564657No Hit
ACAGCC448680.2054191502034825No Hit
CGAGCC441210.20199916033983803No Hit
CCGGAA437320.2002182017629201No Hit
CCTCGG433090.19828158099675994No Hit
GCCATA422660.1935064144267717No Hit
CAGGAC415000.18999943686913892No Hit
ATGGCC414520.18977967848432642No Hit
TCCACG383660.17565104566075623No Hit
CATACG372290.1704455189205102No Hit
GACTTG334520.15315328101557674No Hit
TGCATA295510.1352933339498777No Hit
CTGTTC273160.12506083415704575No Hit
CGAAGC248590.11381195183445601No Hit
ACGAAC230110.10535125401917482No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)