FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24905596
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAC3450241.3853272172245947No Hit
GACTAA2904351.1661435446074047No Hit
ACCGCT2894061.1620119430187497No Hit
TTCCAT2310350.9276429281194475No Hit
ATACCT2194590.881163414037552No Hit
GACCAT2165170.8693508077461788No Hit
CTTCAC2151560.8638861724088032No Hit
TCGGAC2052130.8239634176993798No Hit
AAGCAT2043870.8206468939751531No Hit
AGCCTA2036530.8176997651451505No Hit
TAAGTC2035290.8172018850703271No Hit
ATTGCT2003160.8043011699057513No Hit
ATTCAT2001690.8037109411073721No Hit
CTATTA1988530.7984269880552145No Hit
TATTCT1961460.7875579448088695No Hit
ATTCGA1922730.7720072227944275No Hit
AGTGCA1899630.7627321988198957No Hit
CGAGAG1884510.7566612740365659No Hit
AACATA1855150.7448727587165551No Hit
GGAGGC1830910.7351400062861374No Hit
AGATGC1798450.7221067907790684No Hit
TACTCG1774370.7124382809389505No Hit
TGACAC1749320.7023803003951401No Hit
TTCTGT1748100.7018904506441043No Hit
GCCAAT1728940.6941974004557049No Hit
TGACCA1721150.6910695893404839No Hit
TGATAA1712400.6875563226834644No Hit
TTGAAT1692000.6793653924202416No Hit
GATCAG1666060.6689500624678888No Hit
TCTCGC1659480.66630808594181No Hit
GGTGGT1657660.66557732647715No Hit
GTACGC1647630.6615501190977321No Hit
CTCCGC1633170.6557441949993889No Hit
TAATGA1633080.6557080585423453No Hit
TATGGT1627760.6535719924148774No Hit
GGCGTT1613830.6479788718969022No Hit
TCTTCA1611570.6470714453089177No Hit
GTTACA1605210.6445178023445012No Hit
CGATGT1592850.6395550622438427No Hit
GATTCA1582640.6354555819503376No Hit
CGTCAG1577280.6333034551752947No Hit
AGTCAA1551260.622856003927792No Hit
AACCTT1547050.6211656207705288No Hit
GTAAGA1543370.6196880411936337No Hit
TGTGTG1541870.6190857669095733No Hit
ACCTGC1537040.6171464437148985No Hit
GGATTC1513850.6078352832833231No Hit
AGGTGT1513110.6075381613031866No Hit
AGACGG1502270.6031857258103761No Hit
AATCGG1475970.5926258500298487No Hit
GACCGA1465050.5882412932418882No Hit
TCAAGC1459550.5860329542003331No Hit
CTAGTT1459430.5859847722576083No Hit
TTCACA1454590.5840414339010397No Hit
GCACAT1448400.5815560486888168No Hit
TGCGAC1445370.5803394546350146No Hit
AATTGC1441910.5789502086197816No Hit
CATTAA1440070.5782114188313341No Hit
TCCTCC1439690.5780588426793721No Hit
ATCCAC1427480.5731563300071196No Hit
CATAAC1417710.5692335168369389No Hit
ACGCTA1415690.5684224541344042No Hit
CACCTA1411890.5668966926147843No Hit
TATTGG1404470.5639174424896316No Hit
AGAACG1403510.5635319869478329No Hit
GGATCG1402640.5631826678630779No Hit
GCATAA1402290.5630421371967971No Hit
GTCTCA1401420.562692818112042No Hit
CCGACT1399600.5619620586473819No Hit
ACTTGA1394690.5599906141575572No Hit
GAACCG1389860.5580512909628824No Hit
CAGCCT1367630.5491255860731058No Hit
CAAGCT1364350.5478086129719603No Hit
TCGTGG1355180.5441267095154037No Hit
AACTAG1354160.5437171630022426No Hit
GAGCAC1354150.5437131478403489No Hit
TCCAGA1351260.5425527660530589No Hit
TGCGGA1340210.53811601216048No Hit
CGAATT1337660.537092145877577No Hit
GGTACC1332930.5351929743018397No Hit
GATCCT1332560.5350444133117714No Hit
ACGTAA1330500.5342172899616616No Hit
TTCGTT1329440.5337916828009256No Hit
TCTCTG1316540.5286121239580053No Hit
GTACTT1313050.5272108324570912No Hit
ATGTGA1309420.5257533286896648No Hit
ATCCGG1287930.5171247457800247No Hit
CGTACT1284620.5157957271931979No Hit
AACAAT1276840.5126719312398708No Hit
GCTCAA1276720.5126237492971459No Hit
ACTAGC1276100.5123748092597342No Hit
AGTAGG1269240.5096204082006309No Hit
GCGTAT1268990.5095200291532874No Hit
TGTGAT1259140.5055650946879569No Hit
GCGGCT1258220.505195699793733No Hit
AGGTCA1252580.5029311484856657No Hit
AAGTTG1250870.5022445558018366No Hit
GCCTGG1245510.5000924290267938No Hit
ACAGTG1243780.4993978060191774No Hit
ACCAGG1234430.4956436296485336No Hit
TTAGTA1233600.4953103712113534No Hit
GAGCCA1215150.4879023975174093No Hit
TTACGT1211240.48633246921695833No Hit
CGACAA1180420.473957740260462No Hit
CATGAG1161870.4665096149475805No Hit
CCGCTC1161100.4662004474817627No Hit
GAGAAG1159480.4655499912549774No Hit
GAGTCG1157780.46486741373304213No Hit
TAGAGC1153780.4632613489755475No Hit
GCTGTC1152860.4628919540813238No Hit
CGTCTC1142020.45853951858851316No Hit
TCAATG1132060.45454041734235145No Hit
TACAGT1115740.4479876731317733No Hit
CCGTCG1109250.4453818330627382No Hit
GAGTGA1103060.4428964478505152No Hit
AGGCTT1094620.4395076512122014No Hit
CAGATC1079670.4335049841810652No Hit
CCTTAT1078510.4330392254013917No Hit
CCACTT1078230.4329268008683671No Hit
TGGCAT1076860.43237672368892516No Hit
AGGACT1074720.4315174790436655No Hit
TTAGGC1072050.4304454308180378No Hit
CAGTCC1063070.4268398154374623No Hit
AAGCGC1056760.4243062482825145No Hit
CCTGCT1055790.42391677757882207No Hit
CCATGA1052200.4224753344589706No Hit
ATCAGC1045250.4196847969428237No Hit
GACACG1043720.4190704771730819No Hit
ACACGA1042890.4187372187359018No Hit
TTCTAC1042240.41847623321280886No Hit
CGCTTG1009910.4054952148103583No Hit
CTGAGG990510.39770580073650913No Hit
TTAGCG986300.396015417579246No Hit
CAGTAG984910.3954573100760166No Hit
CCTGAC981920.39425677666978937No Hit
CTATGC975150.39153851206772966No Hit
AGTTAG973530.3908880558409444No Hit
TACGAA972360.39041828189937716No Hit
GGCAGG965260.3875675169548241No Hit
ACTAAT958500.38485326751465815No Hit
GGTAAT952930.38261682233984684No Hit
TCATGT926990.37220149238749395No Hit
CTACCG925700.3716835365032019No Hit
GCGCAG922910.3705633063348494No Hit
TACGGC922360.3703424724306939No Hit
GGCCGT916640.36804579982747654No Hit
CAACGG910770.3656888997958531No Hit
AAGAGG908960.3649621554930868No Hit
GAAGAT906090.36380980402958435No Hit
CTTAGC904340.36310715069818045No Hit
GAATAC902820.36249684609033245No Hit
CTCGTC901720.36205517828202144No Hit
AGAGGT897840.3604972954672516No Hit
GCTTCT893360.3586985029388576No Hit
CCAGCG880980.35372773251441164No Hit
GTGGTG865650.34757248933131335No Hit
AATGTA847070.34011231853275065No Hit
ACAGCC835030.3352780636126917No Hit
CATACG834350.3350050326039176No Hit
ATGCCG827570.33228275283996417No Hit
CTGTAT824510.33105411330048073No Hit
TATAAG821440.3298214585991036No Hit
CCTCGG812420.3261997825709531No Hit
GTCTGC811250.32573000862938595No Hit
TAGCTT809210.3249109156030637No Hit
GGACTA808700.3247061423464831No Hit
CACATT805080.3232526537409504No Hit
AGTTCC798200.32049022235805963No Hit
CAAGGA786870.315941043932456No Hit
GAGATA783080.31441929757472975No Hit
CGAGCC777370.3121266401334062No Hit
AACTCT777220.31206641270500013No Hit
CTGGAG769600.3090068593419728No Hit
GGCCAG766070.3075895071934837No Hit
GTCGAG758460.30453396899235013No Hit
CAGGAC724760.29100287341045766No Hit
CGTGAA722270.29000309809891717No Hit
TCCACG671260.26952175727896655No Hit
GCCATA663230.266297582278296No Hit
AAGGCG655400.2631537105155002No Hit
GTTGGC644410.2587410475942836No Hit
CCGGAA642720.25806248523424213No Hit
TGCATA641260.2574762715977566No Hit
ATGGCC638510.256372102076979No Hit
CGTTGC632000.25375823168415645No Hit
ATTGAA613930.2465028341421743No Hit
CTGTTC599480.24070092520572486No Hit
GACTTG518200.20806568933343333No Hit
ACGAAC494790.19866619534019583No Hit
CGAAGC378660.1520381202682321No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)