FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002210324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117007639
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT8238650.7041121477547291Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT5802550.4959120660489526Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT1958760.16740445467838216TruSeq Adapter, Index 15 (97% over 37bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACATCCTGTAATCTCGTAT1732940.14810486005960688Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)