FastQCFastQC Report
Wed 8 Feb 2023
EGAF00002210330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89778198
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT1890030.21052215817474973TruSeq Adapter, Index 5 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAAACATCGATCTCGTAT1795530.19999621734443812Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTGGTCAATCTCGTAT1658210.18470074438339695Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)