FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002210354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25684813
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTAT10668094.153462203520812TruSeq Adapter, Index 11 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGACTAGTAATCTCGTAT5139012.000797124744494Illumina Single End PCR Primer 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTA2345220.9130765328133789TruSeq Adapter, Index 11 (97% over 38bp)
CGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCACACGTCTGAACTCCAG1841690.7170346149687755No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1234440.48061085747441495Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGCACACGTCTGAACTCCAG1223940.47652283861284095Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACGACTAGTAATCTCGTAT883150.34384131977133725Illumina Single End PCR Primer 1 (95% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTATG551460.21470275060986427TruSeq Adapter, Index 11 (97% over 37bp)
CGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAACTCCAG391050.15224950245890442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGATACGGCGACCACCGAACTCCAGTCACGACTAGTAATCTCGTATGC348200.13556649215238592No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACGAGCTGAAATCTCGTAT325160.12659621076470365Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCCACCACCGAGAACTCCAGTCACGACTAGTAATCTCGTAT259940.10120377360738426Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)