FastQCFastQC Report
Sat 13 May 2023
EGAF00002210355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25684813
Sequences flagged as poor quality0
Sequence length100
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4730631.841800444488344Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1959720.7629878403241636Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1623160.6319532090811796Illumina Single End PCR Primer 1 (100% over 30bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT881300.34312104978144087Illumina Single End PCR Primer 1 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG818730.3187603507177568Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG264330.10291295482665185Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGCAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG258340.10058083739990632Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)