FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002210370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40485300
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCTGAGCCAATCTCGTAT1845590.4558666972950676Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCTCAATGAATCTCGTAT1155370.28538012562584447Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTAT810480.2001911805025528TruSeq Adapter, Index 7 (97% over 37bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCTGAGCCAATCTCGTAT546710.135039137662312Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)