FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002210374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58663284
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACGACTAGTAATCTCGTAT1021350.17410378866617832Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTAT934930.15937225744129838TruSeq Adapter, Index 11 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGAATCTGAATCTCGTAT697340.11887162675720643Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACAAGGTACAATCTCGTAT589020.10040692573569526Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)