FastQCFastQC Report
Sat 13 May 2023
EGAF00002210394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002210394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30466128
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT5287401.73550114409025TruSeq Adapter, Index 10 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGACTAGTAATCTCGTAT4148231.3615875309130192Illumina Single End PCR Primer 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA1941850.637379978184297TruSeq Adapter, Index 10 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGAGCTGAAATCTCGTAT1142030.3748523606281704Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT799850.2625374645573602Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACGACTAGTAATCTCGTAT675020.22156409242421615Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT470260.15435502667093107Illumina Single End PCR Primer 1 (96% over 25bp)
CAAGCAGAACTCCAGTCACGAGCTGAAATCTCGTATGCCGTCTTCTGCTT372710.12233586099290333RNA PCR Primer, Index 25 (96% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTATG352680.1157613465025815TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)