FastQCFastQC Report
Mon 13 May 2024
EGAF00002211692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002211692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402069067
Total Bases60.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT6803200.16920476003691176Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC6406560.15933978825583217Illumina Single End PCR Primer 1 (96% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4800060.11938396643679132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)