FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002215118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002215118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832425
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTACACAGATCTGTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTC31720.3810553503318617No Hit
GTACAGGAATATTGACCTGTTTCCCATCAGCTACGCATCTCTGCCTCGCC31240.37528906508093823No Hit
TTCCTGTACCGATCAATAGTGCGATGTGGGGACGGAGAAGGTTAGCTCAG26090.3134216295762381No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT22650.272096585277953No Hit
ACCATACACTGTCCCCATCCCGGATAACGGGACAAGGTTAGAACCTCAAA22220.2669309547406673No Hit
AGCCTAAACAGTGCTCTACCCCCGATGGTAATACTTGAGGCACTACCTAA18850.22644682704147523No Hit
GTTTAGGCTAGGGGGTCATGGCGACTTACCAAACCTATGCAAACTCCGAA16700.200618674355047No Hit
CCTTAGAATACTCATCTCGCGCACCAGTGTCGGTTTGCGGTACGGTCGTC13630.16373847493768207No Hit
TCATTACTCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT13550.16277742739586148No Hit
ATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACA13110.1574916659158483No Hit
GAGTAATGATGGCCACACTGTCTCCTCCAGAGACTCAGCGAAGTTGAAAT13020.15641048743130012No Hit
CCATACACTGTCCCCATCCCGGATAACGGGACAAGGTTAGAACCTCAAAC11140.13382587019851638No Hit
GCGTTAAAGGGGGTGAAAAGCCCCCTCGCCGAAAGCGCAAGGTTTCCTAC10990.13202390605760278No Hit
ACTAAAGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGCCGAGTTCC10730.1289005015466859No Hit
CGCTTATGCCAATGCACTATCGTCACGATTTCCGACCGTAACTAGCGTAC10200.1225335615821245No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGACTGAAGTCCCAGT10060.1208517283839385No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGTAGGGACGCTAGTTTCTATGGAG9980.11989068084211792No Hit
CCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACA9510.11424452653392198No Hit
CTGTAAGCCTGTGAAGGTGGGTTGTGAAGCCTGCTGGAGGTATCAGAAGT9030.10847824128299846No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGACTGTTTTAGCCTTC8960.10763732468390547No Hit
CTATTGATCGGTACAGGAATATTGACCTGTTTCCCATCAGCTACGCATCT8770.10535483677208157No Hit
ATACATAGGTCATTGTGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT8710.10463405111571612No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC8560.10283208697480255Clontech Universal Primer Mix Long (96% over 25bp)
CTTTAACGCTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCAGGC8540.1025918250893474No Hit
ACTCTATTCGCGCCTAGTTCCACAATCTGCATTTCTCATACGGGGCTATC8540.1025918250893474No Hit
GACTAAGTGCATGTGGTGGATGCCTTGGCGATTACAGGCGACGAAGGACG8330.10006907529206835No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)