FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002216358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732500
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT146622.001638225255973Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130201.7774744027303755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT93661.2786348122866895Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56560.7721501706484643Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT49300.673037542662116Clontech Universal Primer Mix Long (96% over 28bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44800.6116040955631399Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43210.5898976109215017Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39220.5354266211604095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA37310.5093515358361775Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34780.4748122866894198Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.46552901023890786No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33780.461160409556314Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32360.4417747440273037Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA30470.41597269624573374Clontech Universal Primer Mix Long (96% over 28bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30450.4156996587030717Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT29770.4064163822525597Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA27240.37187713310580206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAA27090.3698293515358362Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26650.36382252559726963Clontech Universal Primer Mix Long (96% over 28bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT26420.3606825938566553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG24920.34020477815699657Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.3220477815699659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23050.3146757679180887No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21350.2914675767918089Clontech Universal Primer Mix Long (96% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT21330.2911945392491468Clontech Universal Primer Mix Long (96% over 28bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20790.2838225255972696Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20640.28177474402730374Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT20130.2748122866894198Clontech Universal Primer Mix Long (96% over 28bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA19800.27030716723549486Clontech Universal Primer Mix Long (100% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19160.2615699658703072Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17850.24368600682593855Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT17620.24054607508532422Clontech Universal Primer Mix Long (100% over 23bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC16160.22061433447098977Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA16020.21870307167235495Clontech Universal Primer Mix Long (96% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15950.21774744027303755Clontech Universal Primer Mix Long (96% over 26bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCA15480.2113310580204778Clontech Universal Primer Mix Long (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC15430.21064846416382252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA15360.20969283276450512Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGG14890.2032764505119454Clontech Universal Primer Mix Long (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG14350.19590443686006825Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.18907849829351536No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13770.1879863481228669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATG13320.18184300341296927Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13320.18184300341296927Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT13210.18034129692832765Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA13120.1791126279863481Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT13000.17747440273037543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA12650.1726962457337884Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT12590.17187713310580205Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT12030.16423208191126282Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG11540.15754266211604095Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11470.15658703071672356Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11280.15399317406143345Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11190.15276450511945394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10470.14293515358361775Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10240.13979522184300341Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10150.1385665529010239Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9900.13515358361774743Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATAC9800.13378839590443686Clontech Universal Primer Mix Long (96% over 28bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9790.13365187713310578Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9620.13133105802047781Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG9610.13119453924914676Clontech Universal Primer Mix Long (100% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG9380.12805460750853243Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9260.12641638225255974Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACAT8650.11808873720136519Clontech Universal Primer Mix Long (96% over 28bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8490.11590443686006827Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATACCACTG8060.11003412969283277Clontech Universal Primer Mix Long (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.10948805460750852No Hit
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7760.10593856655290103Clontech Universal Primer Mix Long (100% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7660.10457337883959045Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7620.1040273037542662Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG7620.1040273037542662Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)