FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002216359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732500
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT134091.830580204778157Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA118621.6193856655290102Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84681.1560409556313993Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52860.7216382252559727Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT45020.6146075085324232Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG39550.5399317406143345Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38650.5276450511945393Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37770.5156313993174061Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA35000.477815699658703Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32620.44532423208191124Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30030.4099658703071672Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28650.39112627986348125Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28060.3830716723549488Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA27450.3747440273037543Clontech Universal Primer Mix Long (96% over 28bp)
GCTTGCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGT27340.3732423208191126Clontech Universal Primer Mix Long (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA25400.34675767918088735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25160.343481228668942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24980.34102389078498296Clontech Universal Primer Mix Long (96% over 28bp)
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAA24930.34034129692832765Clontech Universal Primer Mix Long (96% over 28bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG21860.2984300341296928Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG19540.2667576791808874Clontech Universal Primer Mix Long (96% over 26bp)
GCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT19020.2596587030716723Clontech Universal Primer Mix Long (96% over 28bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18690.2551535836177474Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC18580.2536518771331058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA18580.2536518771331058Clontech Universal Primer Mix Long (100% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.2526962457337884No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT17960.24518771331058017Clontech Universal Primer Mix Long (96% over 28bp)
GCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17640.24081911262798633Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16410.22402730375426622Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT15690.21419795221843002Clontech Universal Primer Mix Long (100% over 23bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15110.20627986348122868Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC14690.2005460750853242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCA14480.19767918088737202Clontech Universal Primer Mix Long (100% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC14430.1969965870307167Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13770.1879863481228669Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA13540.18484641638225255Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGG13370.18252559726962458Clontech Universal Primer Mix Long (100% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13270.181160409556314Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG13220.1804778156996587Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12640.17255972696245736Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA12560.17146757679180885Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATG12240.167098976109215Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11750.16040955631399317Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT11310.15440273037542662Clontech Universal Primer Mix Long (96% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11310.15440273037542662Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG11300.15426621160409557Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT11020.15044368600682592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10780.14716723549488053Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10360.1414334470989761Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10140.13843003412969285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT9840.1343344709897611Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9610.13119453924914676Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.13037542662116042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9550.13037542662116042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9490.12955631399317408Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9270.12655290102389077Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG9020.12313993174061433Clontech Universal Primer Mix Long (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATAC8960.12232081911262799Clontech Universal Primer Mix Long (96% over 28bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8730.11918088737201366Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8720.1190443686006826Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8590.11726962457337883Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG8360.1141296928327645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACAT7980.10894197952218429Clontech Universal Primer Mix Long (96% over 28bp)
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7810.10662116040955631Clontech Universal Primer Mix Long (100% over 26bp)
ACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATACCACTG7640.10430034129692833Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATACCACTGCTTCC7400.10102389078498293Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGATACCACTGCTTGC7390.10088737201365187Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)