FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002216829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18167251
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCG192157610.577142353567966No Hit
GATTCA9729085.355284627266943No Hit
TTCACA8550494.706540356601007No Hit
ATACCT8546854.704536751322476No Hit
CAGTCC7709554.243652493159257No Hit
GCCAAT7448584.100003902626765No Hit
TCTCGC6535943.5976494187260366No Hit
AGGTGT6187123.4056445854136106No Hit
GACCAT6134763.3768234940993547No Hit
CTTCAC6015303.311067810974814No Hit
ACTTGA3115921.7151301537034966No Hit
ACTAAT2991631.6467158404978275No Hit
ACAAGT2902741.5977871390668845No Hit
TTCCAT2641931.4542266190960866No Hit
AGTGCA2401071.3216473972864689No Hit
CTTGTA2045631.1259986444839674No Hit
AGAACG2022291.1131513512969025No Hit
CGACAA2016951.1102119963003758No Hit
ACAGTG1932431.0636887220856914No Hit
AATTGC1919161.0563843698752222No Hit
TAGAGC1910111.0514028787294236No Hit
CAGATC1891741.0412912773649685No Hit
CGTACT1867121.0277394196843541No Hit
GACTAA1854771.0209414732036233No Hit
ATTCGA1849171.0178590035443447No Hit
ATTCAT1843941.0149801970589827No Hit
TACGAA1790390.9855040809421305No Hit
GGCTAC1697140.9341754567050349No Hit
CCACTT1635070.9000095831779943No Hit
ATGTGA1562270.8599374776073716No Hit
GCCATA1537250.846165443522523No Hit
TTCTGT1524110.8389326486434299No Hit
CGAAGC1514860.8338410692955143No Hit
TATAAG1419460.7813289968856598No Hit
TCGGAC1333780.7341672110986962No Hit
CGTCTC1318340.7256684018952565No Hit
GCTCAA1315220.7239510259422298No Hit
TTCTAC1262710.6950473684763864No Hit
AGGTCA1236720.6807414066112699No Hit
GCGTAT1183280.651325839005582No Hit
ACCAGG1175350.6469608417916393No Hit
GCGCAG1166520.6421004476681695No Hit
CTAGTT1153780.6350878291933105No Hit
CTACCG1151310.6337282398971644No Hit
CTCCGC1137590.6261761892319316No Hit
TACTCG1130050.6220258640121171No Hit
TGACAC1123430.6183819445220413No Hit
GCGGCT1100090.6055346513349763No Hit
TCCTCC1092140.6011586453008217No Hit
CATGAG1077150.5929075345521455No Hit
AACTCT1017770.5602223473435799No Hit
GTCTCA992810.5464833397193665No Hit
TTAGGC931590.5127853410513237No Hit
AGAGGT867260.47737547084036No Hit
CCTGAC800180.4404518878502862No Hit
CTGTAT789660.434661248418927No Hit
CGCTTG769260.4234322518029833No Hit
TGCGAC763050.42001401312724745No Hit
TGACCA761340.41907275899914626No Hit
GGTAAT739670.4071447022997591No Hit
GCCTGG677780.37307790815462394No Hit
TGATAA674560.3713054881005387No Hit
CTTAGC669100.368300080182742No Hit
GTGGTG668170.36778817004289754No Hit
GGCAGG660920.3637974727161528No Hit
CCGCTC658030.3622066981955608No Hit
GGCCGT606160.33365532297649214No Hit
CGATGT597360.32881144208333996No Hit
TGTGAT588980.3241987464146337No Hit
ATCAGC580840.31971815658846786No Hit
ATTGCT566380.31175877957540193No Hit
TCATGT566300.3117147442945551No Hit
GACACG557560.306903889862038No Hit
TCTTCA554250.3050819301170001No Hit
AGTTAG534770.294359339230795No Hit
TGCGGA527600.29041267718489716No Hit
GTACTT524000.2884310895467894No Hit
AACATA507500.2793488128721291No Hit
GTCTGC502520.2766076166394134No Hit
GATCAG502320.27649752843729636No Hit
CCGGAA488560.26892346013164015No Hit
TTAGCG429490.2364089096363561No Hit
ATTGAA419960.2311632068054765No Hit
AGTTCC412810.22722755357979038No Hit
TACAGT385590.2122445492716537No Hit
TAGCTT352040.193777253366511No Hit
CCAGCG336770.18537201913487075No Hit
TCCAGA327820.18044557209013076No Hit
AAGCGC259930.14307613188148277No Hit
GACTTG229260.12619410608682624No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)