FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24812245
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCA7397892.9815480219544823No Hit
TATAAG6843172.757980988822253No Hit
AGTGCA6506322.622221407212447No Hit
GACTAA6384782.573237528486439No Hit
CTACCG5968262.4053688007675245No Hit
TTCCAT5891402.374392160000032No Hit
GCGTAT5781162.3299624842492084No Hit
TTAGCG5767552.324477289338389No Hit
TAGCTT5326082.1465530426609924No Hit
ATCAGC4977002.0058644431408768No Hit
CCAGCG4815141.940630523356512No Hit
CCACTT4721361.9028346689306026No Hit
CAAGGA3828891.5431453300578002No Hit
AGAACG3812041.5363543282762202No Hit
GGCTAC3743051.5085495085188785No Hit
TTCACA3687871.4863104890347487No Hit
TCTTCA3684921.4851215599394574No Hit
TGCGAC3617341.4578850079869838No Hit
AACATA3521781.4193717658357798No Hit
CCGCTC3468481.397890436758141No Hit
CCGTCG3437611.385448999072837No Hit
ACAAGT3420921.378722481581171No Hit
TGATAA3390911.3666276469541552No Hit
GAACCG3219871.2976939410359685No Hit
TTCTAC3174771.2795174318164277No Hit
AGTTCC3155081.2715818338888722No Hit
CAGTCC3129441.2612482264301357No Hit
TCGGAC3079691.2411976425349662No Hit
GTCTCA3079411.2410847950276165No Hit
TACAGT2976091.199444064815578No Hit
CGACAA2952541.1899527833938444No Hit
GCCATA2925511.179058968666479No Hit
TGTGAT2904621.1706397385645675No Hit
TCATGT2882581.1617570276288984No Hit
TCTCGC2831891.1413275985304836No Hit
ATTGCT2831321.1410978732476647No Hit
CTTAGC2803601.1299259700200446No Hit
GCGGCT2801631.1291320072004771No Hit
GACTTG2792731.1255450685740045No Hit
AACTCT2729991.100259166391433No Hit
ACTTGA2685371.0822761100416347No Hit
CTAGTT2510921.0119680826946535No Hit
GACACG2498441.0069383080813525No Hit
AATTGC2371980.9559715374404856No Hit
GGCCGT2260870.9111912283632537No Hit
GTGGTG2161790.8712593318339392No Hit
CGTCTC2136930.861240085288534No Hit
CAGATC2134420.8602284879905063No Hit
ATACCT2050600.8264467806117504No Hit
GTCTGC2038620.8216185194044311No Hit
GCCAAT1950490.7860997664661138No Hit
ATTCGA1920910.7741782333682422No Hit
CGTACT1895920.7641065933372816No Hit
GTCGAG1885890.7600642344132906No Hit
ATTGAA1883880.7592541505212447No Hit
CCTGAC1860460.7498152625850664No Hit
AAGCGC1839060.7411904888090537No Hit
CTGAGG1761150.7097906698890003No Hit
GGCAGG1752570.706332699842356No Hit
TGACAC1749180.7049664389498008No Hit
CTGTAT1706920.6879345258762357No Hit
ACCAGG1663310.6703585266065203No Hit
TACTCG1652930.6661751082983421No Hit
CCGGAA1610730.6491673768334949No Hit
CATGAG1609120.6485185036662342No Hit
ATTCAT1608970.6484580496444398No Hit
GACCAT1578340.6361133383940067No Hit
ACAGTG1539560.6204839586260735No Hit
GTACTT1496510.6031336543710575No Hit
GTTGGC1492160.6013804877390175No Hit
GATCAG1462950.5896080745615724No Hit
CGAAGC1445640.5826316804464892No Hit
AGAGGT1429160.5759897985853355No Hit
AGGTCA1404170.5659181585543751No Hit
TAGAGC1374480.5539522925071875No Hit
TCCAGA1343400.541426219191371No Hit
CGATGT1340020.5400639885669354No Hit
ATGTGA1319820.5319228469652787No Hit
GCGCAG1161210.46799876431979454No Hit
CGCTTG1157000.4663020214414294No Hit
TTCTGT1125810.45373161517629707No Hit
GGTAAT1081210.435756619362738No Hit
GCCTGG1022890.41225209568904386No Hit
TTAGGC1013510.40847170419282897No Hit
TACGAA1009240.4067507797057461No Hit
AGTTAG935290.376946946961067No Hit
GCTCAA917960.36996249230974465No Hit
CTTGTA910400.3669156096113028No Hit
CTTCAC891670.35936691742323196No Hit
TCCTCC686160.2765408772966735No Hit
ACTAAT276250.11133615680483569No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)